Method for detecting phosphate radical in blood

A phosphate radical and blood technology, applied in the field of phosphate radical detection in blood, can solve the problems of inconvenient measurement work, and achieve the effect of simple detection process, small error and good selectivity

Inactive Publication Date: 2005-04-06
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 3. The use of synthetic receptors to measure phosphate in blood requires a lot of synthetic work and the need to screen re

Method used

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  • Method for detecting phosphate radical in blood
  • Method for detecting phosphate radical in blood
  • Method for detecting phosphate radical in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take a human blood sample and centrifuge it on a TGL-16B high-speed desktop centrifuge to obtain a light yellow clear liquid in the upper layer and place it for 2 hours; take 6ml of the light yellow clear liquid in the upper layer, transfer it to a 10ml ultrafiltration cup, and store it at room temperature and 5×10 5 Under the nitrogen condition of Pa, use PM-10 ultrafiltration membrane with a molecular weight cut-off of 10kD, ultrafilter with Amicon Model 8010 ultrafilter, collect the filtered colorless clear liquid, and store it at -17°C to -20°C for measurement ; prepare the HEPES (10mM) buffer solution of pH7.0, and prepare 2×10 -3 MYbCl 3 solution and 2 x 10 -3 Catechol violet solution of M; add 2ml of HEPES buffer solution to a clean UV cuvette as a blank, draw 2×10 -3 50 μl of catechol violet solution of M was added to the cuvette, at this time the solution turned from colorless to yellow, detected on a UV-visible spectrophotometer, and there was a maximum abso...

Embodiment 2

[0027] Embodiment 2: prepare the HEPES (10mM) buffer solution of pH6.5, all the other are with embodiment 1, obtain the phosphate radical content in the blood and be 2.22mmol / L, the ultraviolet-visible absorption figure sees figure 2 .

Embodiment 3

[0028] Embodiment 3: with Yb (ClO 4 ) 3 The solution, get another person's blood, all the other are the same as embodiment 1, 100 * 2 / 125, the phosphate radical content in the blood is 1.60mmol / L, and the ultraviolet-visible absorption figure sees image 3 .

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Abstract

This invention can qualitative and quantitative measure the phosphate radical content in blood by use of HEPES buffer solvent with PH value 6.5 to 7.5 and with pyrocatechol violet and ytterbium ion as color-developing agent. This method is free of interference of other contents and can be used in clinic.

Description

Technical field: [0001] The invention relates to a method for detecting phosphate radicals, in particular to a method for detecting phosphate radicals in blood. Background technique: [0002] Phosphorus is one of the most abundant elements in the human body, second only to calcium. Eighty percent is combined with calcium and stored in bones and teeth, and the remaining 20 percent is distributed in soft tissues such as nerve tissue. Inorganic phosphate in blood only accounts for a very small part and constitutes the buffer system of blood. Too much inorganic phosphate in the blood may be caused by: hypoparathyroidism, advanced chronic nephritis, excessive intake of vitamin D, multiple myeloma, or fracture healing. If the inorganic phosphate in the blood is too low, there may be diseases such as hyperparathyroidism, renal tubular degeneration, rickets or rickets, secondary parathyroid hyperplasia, long-term diarrhea or malabsorption. Therefore, clinically, the determination ...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/78G01N33/50G01N33/52
Inventor 阴彩霞杨频霍方俊
Owner SHANXI UNIV
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