Method for detecting phosphate radical in blood
A phosphate radical and blood technology, applied in the field of phosphate radical detection in blood, can solve the problems of inconvenient measurement work, and achieve the effect of simple detection process, small error and good selectivity
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Embodiment 1
[0025] Take a human blood sample and centrifuge it on a TGL-16B high-speed desktop centrifuge to obtain a light yellow clear liquid in the upper layer and place it for 2 hours; take 6ml of the light yellow clear liquid in the upper layer, transfer it to a 10ml ultrafiltration cup, and store it at room temperature and 5×10 5 Under the nitrogen condition of Pa, use PM-10 ultrafiltration membrane with a molecular weight cut-off of 10kD, ultrafilter with Amicon Model 8010 ultrafilter, collect the filtered colorless clear liquid, and store it at -17°C to -20°C for measurement ; prepare the HEPES (10mM) buffer solution of pH7.0, and prepare 2×10 -3 MYbCl 3 solution and 2 x 10 -3 Catechol violet solution of M; add 2ml of HEPES buffer solution to a clean UV cuvette as a blank, draw 2×10 -3 50 μl of catechol violet solution of M was added to the cuvette, at this time the solution turned from colorless to yellow, detected on a UV-visible spectrophotometer, and there was a maximum abso...
Embodiment 2
[0027] Embodiment 2: prepare the HEPES (10mM) buffer solution of pH6.5, all the other are with embodiment 1, obtain the phosphate radical content in the blood and be 2.22mmol / L, the ultraviolet-visible absorption figure sees figure 2 .
Embodiment 3
[0028] Embodiment 3: with Yb (ClO 4 ) 3 The solution, get another person's blood, all the other are the same as embodiment 1, 100 * 2 / 125, the phosphate radical content in the blood is 1.60mmol / L, and the ultraviolet-visible absorption figure sees image 3 .
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Abstract
Description
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Application Information
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