Fusarium oxysporum strain and application thereof

A technology of Fusarium oxysporum and Fusarium oxysporum, which is applied to the determination/testing of fungi, microorganisms, microorganisms, etc., and can solve the problems of improving the rapid propagation technology of pathogenic fungi, slow passage, and large quantities

Active Publication Date: 2019-12-27
SHANDONG PEANUT RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, most fungi have the characteristics of long growth cycle and slow passage. Therefore, in order to quickly obtain pure cultures with stable pathogenicity, appropriate selective culture methods must be used to shorten the passage cycle.
In addition, the screening and identification of peanut disease-resistant germplasm must be combined with field experiments, which requires a large number of pathogenic fungi, which undoubtedly increases the difficulty of rapid and mass propagation of pathogenic fungi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Separation, screening and purification of strains

[0020] Two materials Huayu 917 and Huayu 918 with severe rot disease incidence were selected during the harvest period at the Laixi Peanut Production Experiment Base in Qingdao, and three plants with a fruit rot rate of more than 50% were selected respectively. The diseased fruits of 5 plants were mixed and packed into sealed bags, stored in foam boxes with ice packs at low temperature, brought back to the laboratory, and stored in a 4°C refrigerator for later use.

[0021] Five diseased fruits were randomly selected from the two peanut materials as experimental materials, and three groups of repeated experiments were carried out. All experimental steps were performed aseptically. Rinse the diseased fruit 5 times with sterile water to remove the soil and surface attachments, then dry the water with sterile filter paper on the ultra-clean workbench and cut the outer shell with sterilized scissors. Take t...

Embodiment 2

[0028] Embodiment 2: the rapid culture method of Hs-f

[0029]Since the Hs-f strain grows slowly on the PDA solid medium, and the sporulation time is about 15 days, we improved its subculture conditions.

[0030] First, a single colony was picked and inoculated into a 10ml centrifuge tube filled with 5ml PDA liquid medium, and cultured in a shaker at 25°C and 180rpm for 8h. Afterwards, 5 ml of culture solution was taken and transferred into a Erlenmeyer flask filled with 50 ml of PDA liquid medium, and cultured overnight at 25° C. and 180 rpm with shaking. Finally, spread the enriched culture solution on a 9 cm diameter PDA medium plate, dry it on the ultra-clean workbench, repeat the coating twice, and place it in an incubator at 25°C for dark cultivation overnight after drying. And the pH value of the PDA liquid medium used is 6.5. In this way, the obtained pure culture is rapidly subcultured to 50 generations, and the strains with stable pathogenicity are preserved. , se...

Embodiment 3

[0033] Embodiment 3: Hs-f bacterial strain pathogenicity identification method

[0034] The infected peanut seeds, peanut seedling roots, peanut seedling stems, peanut seedling cotyledons and peanut leaves were sterilized and rinsed respectively, then subjected to minimally invasive treatment, placed in a petri dish and inserted with a pathogenic fungus with a diameter of 0.3cm The silk block enables co-cultivation of peanut tissue and pathogenic fungi. Cultivate in the dark in an incubator at 25°C, observe and count the infection and pathogenicity after 5 days: the inoculation points of inoculated fresh seeds and other tissues turn yellow and black until they rot and spread.

[0035] The Hs-f pathogen has strong pathogenicity to peanut tissues and fresh seeds, and the pathogenicity rate to peanut seeds, seedling roots, seedling stems, seedling cotyledons and leaves is more than 50%, and has a strong pathogenicity. Sickness.

[0036] The pathogenicity statistics of the patho...

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Abstract

The present invention discloses a fusarium oxysporum strain and belongs to the technical field of pathogenic microorganism isolation. The fusarium oxysporum is fusium oxysporum Hs-f (Fusarium sp.), isdeposited on August 29, 2019 in China General Microbiological Culture Collection Center and has a deposit number of CGMCC NO:18130, the address is No.3, No.1 courtyard, Beichen West Road, Chaoyang District, Beijing, and a unit asking for deposition is Peanut Institute of Shandong province. The peanut rot pathogen fungus is isolated and purified from peanut rot disease seed kernels. Inoculation experiments prove that the fusarium oxysporum strain has high pathogenicity, is also free of tissue specificity, and has pathogenicity to peanut roots, stems, leaves and seed kernels.

Description

technical field [0001] The invention belongs to the technical field of isolation of pathogenic microorganisms, and in particular relates to a strain of Fusarium oxysporum and its application. Background technique [0002] Peanut is a temperature-loving legume that blooms on the ground, forms fruit needles and drills into the ground to bear fruit. It is an oil-bearing and economic crop widely planted around the world. However, because of its underground fruiting characteristics, it is susceptible to soil-borne diseases. There are many kinds of peanut soil invasive diseases that have been reported, including fungal diseases, bacterial diseases and nematode diseases. Among them, fungal diseases mainly include stem rot, root rot, and fruit rot. These fungal diseases often lead to the death of peanut seedlings or the rot of underground roots and fruits. There are many reports of large-scale peanut rot and yield reduction or even crop failure caused by fungal diseases, and it h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/18C12Q1/04C12R1/77
CPCC12Q1/04C12Q1/18C12N1/145C12R2001/77
Inventor 王冕张朝昕迟晓元陈娜陈明娜王通潘丽娟许静禹山林
Owner SHANDONG PEANUT RES INST
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