A strain of Beauveria bassiana and its application in controlling southern blueberry grubs

A Beauveria bassiana and blueberry technology, applied in the fields of application, fungi, and microbial-based methods, can solve the impact of strain differences on the virulence of Beauveria bassiana, the difference in pathogenicity, stability and timeliness of Beauveria bassiana to pests Poor performance and other problems, to achieve good development potential and production value, to solve the effects of pesticide residues and strong toxicity

Active Publication Date: 2021-01-08
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] From 2015 to 2017, surveys on blueberry pests in southern China found that the damage rate of grubs to blueberries was more than 40%, especially in Guizhou Province, the province with the largest blueberry planting area in the south. The damage rate of several dominant pests is as high as 60%, resulting in an annual economic loss of up to 20 million yuan in Majiang County. Faced with this tense situation, fruit farmers have to use a large amount of the chemical pesticide phoxim to control it, causing grubs to develop resistance. nature, and pollute the environment, therefore, it is imminent to explore and develop efficient, safe and economical green prevention and control
[0005] Although there are some reports on the screening of Beauveria bassiana grubs and field control effects at present, the stability and timeliness are not good. In addition to the strain difference affecting the virulence of Beauveria bassiana, the Beauveria bassiana of different geographical populations is harmful to pests. There are also significant differences in the pathogenicity of

Method used

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  • A strain of Beauveria bassiana and its application in controlling southern blueberry grubs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Preservation of Beauveria bassiana bacterial strains and strain morphological characteristics

[0017] The Beauveria bassiana strain MJ1015 is Beauveria majiangensis, which was isolated from the infested grub in the blueberry orchard of Majiang County, Guizhou Province. Committee General Microbiology Center (CGMCC), deposit number: CGMCC NO.15090, deposit address: Beijing.

[0018] Cultivated on PDA medium for 13 days, the diameter of the colony can reach 4.81mm, no odor is produced, the aerial hyphae are separated, branched, transparent, smooth, and 1.6-2.2μm wide. Sporogenous cells solitary or clustered laterally, cylindrical at base, sometimes subspherical, 3.8-12.9 (-16.2) μm long, 1.2-1.5(-2.3) μm wide, with an indistinct tooth less than 1 μm at the top axis. Conidia are oval, transparent, septate, smooth surface, 2.4-3.8 μm long, 1.6-2.3 μm wide, Q = 1.6-2.6 (L m =2.1, W m =1.4 , Q m =1.6).

Embodiment 2

[0019] Embodiment 2 The cultivation method of Beauveria bassiana and its biological characteristics

[0020] Inoculate the Beauveria bassiana strain MJ1015 in the improved PDA medium (potato 200g / L, glucose 18g / L, agar 18g / L, add distilled water to 1L, and use KH 2 PO 4 adjusted to pH 5.0), placed in climate chambers at 16°C, 19°C, 23°C, 26°C, 29°C, and 32°C (humidity is 100%), and observed and recorded mycelial growth and sporulation every 2 days quantity situation. The results showed that the most suitable temperature for bacterial colony growth and sporulation of strain MJ1015 was 26°C, and the colony diameter and sporulation yield on the 14th day at this temperature were 5.12 cm (see Table 1) and 120.45*106 spores / ml, respectively. (See Table 2).

[0021] temperature day 2 day 4 day 6 day 8 day 10 day 12 day 14 16℃ 0.09 0.25 0.28 0.67 1.07 1.41 1.73 19℃ 0.11 0.46 0.57 1.16 1.71 2.25 2.68 23℃ 0.25 0.56 0.89 2.01 2.48...

Embodiment 3

[0029] Application of Example 3 Beauveria bassiana strains in the control of blueberry pests

[0030] The bacterial strain used in the present invention is isolated from the infested grub body of the blueberry orchard in Majiang County, Guizhou Province. =4-6) The germination rate is high. It is specially used to prevent and control blueberry grub pests, especially the blueberry pests, the white star beetle, the thick ribbed beetle, and the blue flower beetle. It has the characteristics of strong toxicity and fast insecticidal speed.

[0031] The optimum soil pH range for blueberry growth is 4-6, so the acid resistance of the strain is the primary criterion for testing whether it can control blueberry grubs in the south, and it is also the key to applying the strain for field control.

[0032] The application of described Beauveria bassiana bacterial strain in the prevention and control of blueberry pests, Beetle spp.

[0033] Three strains CH1, CH2, and SWS1 (collected from...

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Abstract

The invention provides a strain of Beauveria bassiana efficiently parasitizing grubs, a cultivation method of the strain and an application of the Beauveria bassiana strain in preventing and treating southern blueberry grubs. The Beauveria bassiana strain was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC) on December 29, 2017. The preservation number is: CGMCC NO.15090. It is specially used to control blueberry grub pests and has strong toxicity. , Insecticidal speed and so on.

Description

technical field [0001] The invention belongs to the technical field of pest biological control and relates to a strain of Beauveria bassiana ( Beauveria majiangensis ) strains and their application in the control of southern blueberry grubs (beetle beetle, beetle thick stenotic ribbranch, and blue beetle). Background technique [0002] Grubs are the collective name for the larvae of scarabs of the order Coleoptera. This kind of pest has wide distribution, many types, mixed feeding habits, strong adaptability, hidden life, and different lengths of life history. [M]. Beijing Forestry Press, 1979; Analysis on the occurrence and control status of underground pests in my country [M]. China Agricultural Press, 2005:389-393.). [0003] From 2015 to 2017, surveys on blueberry pests in southern China found that the damage rate of grubs to blueberries was more than 40%, especially in Guizhou Province, the province with the largest blueberry planting area in the south. The damage rat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 刘曼黄振兴秦华军杨广明聂飞杨丰文光忠张树红
Owner GUIZHOU INST OF BIOLOGY
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