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Method for preparing high efficiency biological weed control bacterial agent and its usage

A herbicide and biological technology, which is applied in the field of preparation and application of high-efficiency biological herbicides, can solve the problems of plant leaf necrosis, infection, plant dwarf, etc., and achieves the effects of low cost, convenient use and simple strains

Inactive Publication Date: 2003-10-08
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The phytotoxin phaseolotoxin produced by Pseudomonas syringae pv. phaseolicola can make the leaves of Kudzu appear chlorosis and cause local necrosis; Green, serious cause necrosis of plant leaves

Method used

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  • Method for preparing high efficiency biological weed control bacterial agent and its usage

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preparation example Construction

[0021] Further illustrate the preparation method of biological herbicide bactericide below by example, concrete steps are as follows:

[0022] 1) Take samples from 10 cm of soil, above-ground stems and leaves from the rhizosphere of Amaranthus retroflexus, remove the excess soil at the roots of the weeds, rinse twice in 200ml sterile water, drop into 100ml 1.0% Tween-80 solution and shake the flask at 500rpm for 10min , with sterile phosphate buffered saline (6MmK 3 PO 4 -KH 2 PO 4 , 0.2MNaCl, PH7.2) do 10-fold serial dilution, get 0.1ml and smear it on NPC medium plate, NPC medium plate refers to adding penicillin, novobiocin, cycloheximide in Davis’ minimal medium, 24 Incubate at ℃ for 22 hours to observe the results, select colored colonies and store them on nutrient agar. Further isolate and purify the single colony screened out: ①Antibacterial test, connect a ring of bacteria to be tested with an inoculation needle, puncture and inoculate it on an E. The bases were c...

Embodiment 1

[0026] Embodiment 1: Amaranthus retroflexus

[0027] Spray fungal agents at the stage of 4 true leaves from the budding of Amaranthus retroflexus seeds, and the dose is 500L / ha, which shows a good control effect (see Table 3). New leaves wilted after 1 day, old leaves began to wilt after 2 days, leaves basically withered after 4 days, and the control effect reached more than 90%. After 3 weeks, the control effect decreased slightly, and part of the stems and leaves of Amaranthus reflexae began to recover, but the growth was poor, and it had no effect on the protected plants.

Embodiment 2

[0028] Example 2: Cassia

[0029] Spraying fungicides at the stage of cassia seed germination to the fourth round of true leaves, the dose is 500L / ha, shows a good control effect. After 2 days, the new leaves began to wilt, and after 3 days, some old leaves began to wilt, and after 7 days, the leaves basically withered, and the control effect reached more than 85%. After 3 weeks, the control effect declined, and a small part of the stems and leaves of Cassia Cinnamomi began to recover, but the growth was extremely poor, and did not affect the protected plants.

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Abstract

A process for preparing the efficient bacterial herbicide includes sampling the soil near the root of weeds such as crabgrass herb, caper euphorbia, cassia, etc. or their stem or leaves, separating with NPC culture medium, and the chlorella pyrenoidosa and glutamine synthetase depressants, and screening the bacterial strains with high herbiciding activity and broad spectrum. Its advantage is highherbiciding effect.

Description

technical field [0001] The invention relates to a preparation method and application of a bio-herbicide bacterial strain Xanthomonas campestris pv. retroflexus bacterial agent. Background technique [0002] Chemical herbicides can effectively control many harmful weeds, but their extensive use has also caused a series of environmental problems, and the development of new chemical herbicides is difficult, with low success rate and high cost. In contrast, microbial herbicides have low toxicity, easy degradation, and good environmental compatibility, and have great development potential. [0003] The research on microbial herbicides began in the 1960s, and a number of commercialized fungal herbicides were launched successively. my country’s Shandong Academy of Agricultural Sciences developed the culture of “Lubao No. 1” Cuscuta spores to control soybean fields. Cuscuta: These fungal herbicides are all spore preparations, with strict requirements for ac...

Claims

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Application Information

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IPC IPC(8): A01N63/00
Inventor 李永泉庄晓峰孙自玲徐凌李明智
Owner ZHEJIANG UNIV
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