Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection

An avian influenza virus and influenza virus technology, which is applied in the cultivation devices of tissue cells/viruses, biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problems of economic and foreign trade losses, and achieve the effect of low price.

Inactive Publication Date: 2003-09-17
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, even if the laboratory proves that the isolate is of low pathogenicity, the chickens are treated as strictly as highly pathogenic influenza, thus causing great losses to the economy and foreign trade

Method used

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  • Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection
  • Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection
  • Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection

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Embodiment Construction

[0023] refer to figure 1 and figure 2 The cell culture device used in the method of the present invention consists of a cell culture bottle 1 and a bacteria culture chamber 3. The bacteria culture chamber is placed in the cell culture flask; the cell culture flask can be a conventional Kjeldahl cell culture flask; the bacteria culture chamber A sealing ring 33 is sandwiched between the a wall 31 and the b wall 32, and a number of fixing screws 35 are fixed to form a closed structure, so that bacteria cannot contact the cells in the cell culture bottle; there are openings (holes) 36 on the b wall , the opening has a microporous filter membrane 34 with a pore size not greater than 0.22 microns to seal as an isolation barrier, so that the culture solution in the cell culture bottle can enter the bacterial culture chamber, and the enzymes secreted by bacteria can enter the cell culture bottle through the microporous filter membrane CEF reaches CEF in the culture medium. The a-w...

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Abstract

A method for determining the pathogenic affection of concurrent bacterial infection to avian influenza virus includes such steps as inoculating the bacterium able to secrete subtilisinoid and / or the bacterium able to secrete tryptase and the low-pathogenicity avian influenza virus in culture medium of CEF in a particular mode, culturing, and observing if CPE presents. Its cell culture equipment is composed of cell culture bottle and bacteria culture chamber. Its advantages are simple method, short time, high correctness and low cost.

Description

technical field [0001] The invention relates to a method for identifying the influence of concurrent bacterial infection on the pathogenicity of avian influenza virus infection and an in vitro homogeneous cell culture device used therein. Background technique [0002] Existing bacterial culture is carried out separately from cell culture. If bacterial contamination occurs in the cell flask during the cell culture process, the cell culture and subculture will not be able to go on for a long time. The main reasons are as follows: a) Bacteria encroach on cells. b) The effects of toxins secreted by bacteria during growth on the cell membrane or different parts of the cell. c) The enzymes secreted by bacteria during the growth process affect a certain link of cell growth. d) During the process of high-speed division, bacteria occupy most of the growth space and absorb a large amount of nutrients, which in turn affects the growth of cells....

Claims

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Application Information

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IPC IPC(8): C12M3/00C12Q1/00
Inventor 杨德威刘福安
Owner SUN YAT SEN UNIV
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