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31results about How to "Mild disease" patented technology

Recombinant H7N9 subtype avian influenza virus-like particle, and preparation method and application thereof

The invention discloses a recombinant H7N9 subtype avian influenza virus-like particle, and a preparation method and application thereof. Recombinant baculoviruses for expressing HA protein, NA protein and M1 protein of an H7N9 highly pathogenic avian influenza virus are respectively constructed on the basis of a recombinant baculovirus insect cell culture system. The three recombinant baculovirusstrains are used for co-infecting suspended insect cells, so that the H7N9 subtype avian influenza virus-like particle capable of being self-assembled in the cells can be obtained. After the concentration and purification with cane sugar with different gradient concentrations through an ultrafiltration tube, the H7N9 subtype avian influenza virus-like particle is mixed and emulsified with an adjuvant to prepare a vaccine. When a chicken is immunized by the prepared vaccine, the body can be induced to generate a specific antibody; and the advantages of high immunogenicity, good safety, high hereditary stability and the like are realized. After the attack by a lethal dose of H7N9 subtype highly pathogenic avian influenza viruses, the complete clinical protection can be provided, and virus expelling of the chicken is obviously inhibited. The invention provides a novel method for preventing H7N9 subtype avian influenza virus infection, and also lays a foundation for the development of a novel influenza virus vaccine.
Owner:YANGZHOU UNIV

Medicament for preventing and treating atherosclerosis and preparation method of granular formulation

The invention relates to a medicament for preventing and treating atherosclerosis and a preparation method of a granular formulation, which are applied to the technical field of medicine. The medicament comprises the components of astragalus mongholicus, hawthorn, polygonum multiflorum, semen cassia, rhizoma alismatis, seaweed, ligusticum wallichii and angelica. The preparation method of the granular formulation comprises the following steps of: adding water to distill ligusticum wallichii and angelica; keeping distillate and decoction; heating and refluxing astragalus mongholicus, polygonum multiflorum and semen cassia twice by using ethanol; filtering; decompressing a filtrate; and recycling ethanol to obtain an ethanol extract of the medicament; adding water to decoct hawthorn, rhizoma alismatis, seaweed, astragalus mongholicus, polygonum multiflorum and semen cassia together; finely filtering; concentrating by using a triple-effect energy-saving concentrator to a relative density; drying a concentrated solution by using a spray drying method to prepare extractum powder; granulating; checking; and sub-packaging in an aluminum plastic film bag to obtain a finished product. The medicament provided by the invention has the advantages of simplicity in components, clear compatibility, less medicament taste, low price, and remarkable curative effect; and animal experiments show that the medicament has the effects of regulating blood lipid, protecting vascular endothelial cells, inhibiting expression of inflammatory factors and remarkably preventing and treating atherosclerosis.
Owner:FIRST AFFILIATED HOSPITAL OF LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE

Establishment of mouse tuberculosis natural dormant infection model preparation and drug evaluation methods

The invention relates to the technical field of animal model building, and in particular to a mouse tuberculosis dormant infection model establishment method. An incubation period of a mouse tuberculosis dormant infection model built by the method provided by the invention can be controlled to be within 20 weeks. In addition, the model built by the method provided by the invention accords with natural dormant infection characteristics of human. The condition that in the early phase of incubation (within 8 weeks), the mouse tuberculosis dormant infection model can be used for quickly and effectively evaluating antituberculosis drugs and vaccines without the need of using relapse in a later period of incubation is discovered for the first time. In addition, a model preparation technology in the invention can shorten the incubation period, stabilization in the incubation period is realized, and relapse conditions are relatively coincident, thus facilitating the research on incubation and relapse mechanisms. In addition, any intervention factor is not used, and an infection process of the model is the same as the natural dormant infection process of human, thus facilitating the research on an immunologic mechanism in the early phase.
Owner:INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI

Avian influenza virus-like particle antigen, vaccine, preparation method of avian influenza virus-like particle antigen and application of avian influenza virus-like particle antigen or vaccine

The invention discloses an avian influenza virus-like particle antigen, a vaccine, a preparation method of the avian influenza virus-like particle antigen and application of the avian influenza virus-like particle antigen or the vaccine. A strain of recombinant baculovirus based on tandem expression of HA, NA and M1 genes of an H7N9 subtype avian influenza virus transfects insect cells and performs self-assembly to form the avian influenza virus-like particle antigen; and the virus-like particle antigen prepared according to the invention is used for preparing the vaccine. After a chicken group is immunized by the vaccine prepared according to the invention, HI antibodies, neutralizing antibodies and IgY antibodies with the higher titer can be generated, 100-percent clinical protection onthe H7N9 subtype highly pathogenic avian influenza virus can be provided, and virus expelling can be effectively inhibited. The rely on the chicken embryos is avoided; the production cost is low; theperiod is short; and higher protection capability can be induced only by once low-dose immunization. A foundation is laid for industrial production of VLP vaccines.
Owner:YANGZHOU UNIV

A kind of recombinant h7n9 subtype avian influenza virus-like particle and its preparation method and application

The invention discloses a recombinant H7N9 subtype avian influenza virus-like particle and its preparation method and application. Based on the recombinant baculovirus insect cell culture system, the expression of H7N9 highly pathogenic avian influenza virus HA protein and NA protein is respectively constructed. , M1 protein recombinant baculovirus. Three strains of recombinant baculoviruses were co-infected in suspended insect cells, and H7N9 subtype avian influenza virus-like particles self-assembled in the cells could be harvested. After being concentrated and purified by ultrafiltration tubes and different gradient concentrations of sucrose, it is mixed with adjuvant and emulsified to prepare the vaccine. The prepared vaccine immunized chickens can induce the body to produce specific antibodies, and has the advantages of strong immunogenicity, good safety, high genetic stability and the like. After challenge with a lethal dose of highly pathogenic avian influenza virus of H7N9 subtype, it can provide complete clinical protection and significantly inhibit the shedding of chickens. The invention provides a new method for preventing H7N9 subtype avian influenza virus infection, and also lays a foundation for the development of new influenza virus vaccines.
Owner:YANGZHOU UNIV

Medicament for preventing and treating atherosclerosis and preparation method of granular formulation

InactiveCN102631502BMild diseaseTo achieve the effect of anti-arteriosclerosisGranular deliveryCardiovascular disorderInflammatory factorsSemen
The invention relates to a medicament for preventing and treating atherosclerosis and a preparation method of a granular formulation, which are applied to the technical field of medicine. The medicament comprises the components of astragalus mongholicus, hawthorn, polygonum multiflorum, semen cassia, rhizoma alismatis, seaweed, ligusticum wallichii and angelica. The preparation method of the granular formulation comprises the following steps of: adding water to distill ligusticum wallichii and angelica; keeping distillate and decoction; heating and refluxing astragalus mongholicus, polygonum multiflorum and semen cassia twice by using ethanol; filtering; decompressing a filtrate; and recycling ethanol to obtain an ethanol extract of the medicament; adding water to decoct hawthorn, rhizoma alismatis, seaweed, astragalus mongholicus, polygonum multiflorum and semen cassia together; finely filtering; concentrating by using a triple-effect energy-saving concentrator to a relative density; drying a concentrated solution by using a spray drying method to prepare extractum powder; granulating; checking; and sub-packaging in an aluminum plastic film bag to obtain a finished product. The medicament provided by the invention has the advantages of simplicity in components, clear compatibility, less medicament taste, low price, and remarkable curative effect; and animal experiments show that the medicament has the effects of regulating blood lipid, protecting vascular endothelial cells, inhibiting expression of inflammatory factors and remarkably preventing and treating atherosclerosis.
Owner:FIRST AFFILIATED HOSPITAL OF LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE

Vine guiding method for vine cowpea

The invention provides a vine guiding method for vine cowpea. According to the vine guiding method, vines of a row of vine cowpea are continuously guided with a sewing thread. The method specifically comprises steps as follows: a, a tightened steel wire is fixed over each row of vine cowpea; b, when vines of the vine cowpea begin to grow out, one end of each sewing thread is tied to the steel wire over the first vine cowpea plant in each row, then each sewing thread is guided to the base of the first vine cowpea plant in each row, winds around the stem of the first vine cowpea plant by one circle and is threaded between a vertical sewing thread and the plant and tensioned; c, each sewing thread is guided to the base of a second vine cowpea plant in each row in the horizontal direction, winds around the stem by one circle and is threaded from the position below the horizontal sewing thread and tensioned; e, each sewing thread is guided to the position over the third vine cowpea plant along the corresponding steel wire, winds around the steel wire by one circle and is threaded between the horizontal sewing thread and the steel wire and tensioned; f, each sewing thread is continuously guided to the last plant in each row with the method and tied to the steel wire or the base of the cowpea after being shorn off; g, vines of each row of the vine cowpea are guided with the same method. The method is simple, low in cost, good in commodity performance, economical and practical, and diseases are reduced.
Owner:宜兴市蔬菜协会 +1

Method for synchronously preventing and controlling persimmon fruit chap and sunburn

The invention discloses a method for synchronously preventing and controlling chap and sunburn of persimmon fruits. The method comprises the following steps of (1) bagging at a proper period; (2) spraying chlorin iron on young fruits; (3) effectively applying a liquid fertilizer; (4) adjusting the microdomain ecology of a tree body; and after first physiological fruit drop of persimmon trees, spraying the chlorine iron with the mass ratio being 0.03 mg/kg and GA4 + 7 with the mass ratio being 10 mg/kg on a whole plant, particularly the surfaces of the young fruits, and spraying until water drops. According to the method for synchronously preventing and controlling dehiscence and sunburn of the persimmon fruits provided by the invention, through scientific application of comprehensive technical measures, the proportion of fruit epidermis and cross ditch dehiscence and fruit sunburn is remarkably reduced, harm of persimmon fruit moths and wasps is effectively avoided, and the appearance quality of the persimmon fruits is improved. Meanwhile, the inherent quality of the persimmon fruits is improved, the average single fruit quality of the fruits is improved by 7.85 percent compared with a control group, and the soluble solid content of pulp is improved by 4.94 percent compared with the control group.
Owner:INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI

Andrographis paniculata plug seedling cultivation method through sand culture

The invention discloses an andrographis paniculata plug seedling cultivation method through sand culture. The andrographis paniculata plug seedling cultivation method comprises the following steps of: sowing andrographis paniculata seeds which are soaked for pregermination in a matrix of seedling cultivation plug, then pressing the seedling cultivation plug into a seedling cultivation tank which is filled with river sand and is watertight at bottom and the periphery, and irrigating to maintain sand bed to be humid so that the seeds absorb water for germination and growing; and when 8-10 pieces of euphylla grow out, transplanting the seedlings with the matrix into a large field. Compared with a conventional andrographis paniculata seedling cultivation technique, the andrographis paniculata plug seedling cultivation method has the advantages of low cost, less sowing quantity, convenience in management, saved labor and fertilizer and the like. Compared with a conventional seedling cultivation technique through nutritional soil, the cultivated seedlings have the advantages of good uniformity, robust plants, developed root systems, transplanting with the matrix, fast reviving and the like. Compared with a floating seedling cultivation technique, the andrographis paniculata plug seedling cultivation method has the advantages of convenience and uniformity in fertilizer and water management, and the cultivated seedlings have the advantages of unserious diseases, developed root systems and the like. The andrographis paniculata plug seedling cultivation method is beneficial to strong andrographis paniculata seedling cultivation and industrial production of the seedlings, thus providing effective technical support for andrographis paniculata GAP (Good Agricultural Practices) planting.
Owner:CROP RES INST GUANGDONG ACAD OF AGRI SCI
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