31results about How to "Mild disease" patented technology

The multifunctional slow releasing lawn fertilizer is prepared through mixing cake residue, turf, humic acid, nitrogen fertilizer, phosphate fertilizer, potash fertilizer, trace elements, and plant growth regulator. It has perfect nourishing and curing function, slow releasing function, and fast, stable and lasting fertilizer effect. Test shows that lawn with the fertilizer applied has early regreening in spring, no excessive growth in summer, fast restoring in autumn, less diseases and pests and long green period, and is environment friendly.

The invention discloses a breeding method of the japonica rice variety Zhendao No.12 characterized in good quality, good taste, rice blast resistance and high production. The rice bred by the method has the advantages of good quality, good taste, high commodity value; excellent disease resistance, good yield stability and fertility, coordinated grain structure, auto-regulation; good plant type and maturation appearance. The breeding method of the invention includes the following steps: (A) selecting the self-selected Zhenxiang 24 of the late-maturing middle japonica rice characterized in early mature period, high production, rice blast resistance and good taste as the female parent, crossbreeding with the strain Zhen 511 characterized in rice blast resistance, good quality and appearance and good taste and obtaining 60 grains of F0 seeds; (B) fry soaking, sprouting and seed sowing of the F0 seeds, multiple crossing with the Wuyujing No. 8 variety with high production but bad quality, self-breeding for eight generations and obtaining the japonica rice characterized in extra quality, good taste, rice blast resistance and high production, that is the Zhendao No.12.

The invention provides a herba andrographis planting method. The method comprises the following steps of seedling cultivation, seedling management, field soil preparation, transplanting planting, field management and harvesting. According to the herba andrographis planting method, occurrence of diseases of herba andrographis is low, the survival rate is high, the plant uniformity is good, growth is fast, reviving is fast, plants are healthy and strong, root systems are developed, leaves are thick, the leaf color is dark green, the yield is high, the planting cost is low, and the economic benefit is high.

The invention relates to the technical field of agricultural planting, in particular to a double-row strip planting secondary ridging cultivation method of amorphophallus. The double-row strip planting secondary ridging cultivation method of the amorphophallus comprises the following steps of A, land selection; B, soil disinfection; C, seed selection; D, amorphophallus seed disinfection; E, seeding; F, fertilization; G, earthing up and management of moisture in the soil; H, weeding; I, covering; J, prevention and control of diseases and insect pests; K, digging of amorphophallus corms. The double-row strip planting secondary ridging cultivation method of the amorphophallus has the advantages that the emergence rate is high, the utilization rate of fertilizers is high, one-time fertilization is achieved, the drainage effect is good, the ventilation and light permeability are good, the disease occurrence rate is low, the labor input is less, the expansion rate of the commercial amorphophallus corms is high, planting saves time and labor, and mechanized harvesting can be achieved during digging.

The invention discloses a recombinant H7N9 subtype avian influenza virus-like particle, and a preparation method and application thereof. Recombinant baculoviruses for expressing HA protein, NA protein and M1 protein of an H7N9 highly pathogenic avian influenza virus are respectively constructed on the basis of a recombinant baculovirus insect cell culture system. The three recombinant baculovirusstrains are used for co-infecting suspended insect cells, so that the H7N9 subtype avian influenza virus-like particle capable of being self-assembled in the cells can be obtained. After the concentration and purification with cane sugar with different gradient concentrations through an ultrafiltration tube, the H7N9 subtype avian influenza virus-like particle is mixed and emulsified with an adjuvant to prepare a vaccine. When a chicken is immunized by the prepared vaccine, the body can be induced to generate a specific antibody; and the advantages of high immunogenicity, good safety, high hereditary stability and the like are realized. After the attack by a lethal dose of H7N9 subtype highly pathogenic avian influenza viruses, the complete clinical protection can be provided, and virus expelling of the chicken is obviously inhibited. The invention provides a novel method for preventing H7N9 subtype avian influenza virus infection, and also lays a foundation for the development of a novel influenza virus vaccine.

The invention discloses an organic planting method for flue-cured tobacco obtained after soil improvement. The planting method comprises the steps of 1 field plot selection, 2 flue-cured tobacco variety selection, 3 soil improvement and 4 flue-cured tobacco planting. Due to the comprehensive soil improvement mode of fallowing, shallow ploughing and deep ploughing, the purpose of effectively improving the deteriorated soil is achieved, the land soil structure, beneficial microbial communities and nutrient balance are better improved, the problems of original growth imbalance, nutrient imbalance, tobacco leaf quality decrease and the like are solved due to the planted flue-cured tobacco, tobacco plants grow well, and the tobacco leaf yield and quality are obviously improved. No chemical fertilizer or chemical pesticide is needed in the whole process, the field production and management mode is adopted, the produced flue-cured tobacco is good in growth and development in the whole growing period, and tobacco leaves are delicate and pretty and have little diseases.

The invention discloses an anti-virus pig feed and a production method thereof. According to the feed, viruses can be resisted and defended by a multiple design; firstly, the viruses are resisted by adopting radix saposhnikoviae, honeysuckle flowers, herba houttuyniae, herba ephedrae, herba schizonepetae and cortex moutan radicis, which have an anti-virus effect; secondly, the viruses are resisted by the strong sterilization and virus-killing effect of an antitoxic agent; thirdly, the feed is subjected to acidification treatment by malic acid so that the pH value of the feed is reduced, and the viruses are resisted by inhibiting the reproduction of bacteria and viruses; and the three manners are combined and are cooperated, so that the anti-virus capability can be enhanced and the defending effect is remarkable; and the immunity of pigs is strong, the morbidity quantity is few and the morbidity is low.

The invention discloses a vegetable cultivation nutrient bed and a vegetable cultivation method, and belongs to the technical field of vegetable soilless cultivation production. The vegetable cultivation nutrient bed mainly comprises surrounding walls on the two sides and a bed body in the middle, the bed body is composed of a water-retention film layer, a cobblestone layer, a water-permeable film layer and nutrient soil, and the water-retention film layer, the cobblestone layer, the water-permeable film layer and the nutrient soil are sequentially arranged from bottom to top. The water-retention film layer is a right-angle U-shaped plastic film located at the bottommost layer, and the nutrient soil is formed by uniformly mixing and fermenting the raw materials comprising, by weight, 5-15 parts of river sand, 25-35 parts of chaff, 25-35 parts of biogas slurry and 25-30 parts of sawdust. Vegetables cultured through the bed and the method are high in quality, high in yield, environmentally friendly, clean and healthy, save cost and have the advantages of being capable of maturing early, little in disease, free of inorganic fertilizers and free of pollution, saving water and labor and the like.

The invention relates to a cultivation method of sorghum. The cultivation method comprises the following contents: A, seed cultivation; B, a sowing cultivation method in spring and in summer: a) sowing; b) controlling the density; c) fertilizing; d) performing field management and harvesting; C, a regeneration cultivation method. Parent seeds of the sorghum provided by the invention are systemically selected from Honeyingzi sorghum plants; the yield of the sorghum is significantly higher than that of the Honeyingzi; the main reason for yield increase is significant increase in the number of grains per spike, with an average increase of 150 grains per spike; the thousand seed weight is also significantly increased by about 1 gram. A sorghum variety cultivated by the cultivation method has the plant height being about 260cm, which is about 20 cm lower than the plant height of the Honeyingzi; the lodging resistance of the variety is enhanced, so that mechanized harvesting is facilitated; the variety is high in amylopectin content, strong in glutinousness and moderate in tannin content; when the variety is applied to wine brewing, the wine yield is high and the wine quality is good.

The invention provides a mulberry cultivation method for fruit and leaf utilization. The method comprises the steps of garden construction, seedling raising, planting, grafting and pruning, and further comprises water and fertilizer management measures and pest control measures. The grafting takes Yunsang No.1 as a rootstock and a morus macroura variety as scion; a mulberry planting area is ridgedand maintains a ridge structure, and two rows of mulberry trees are planted on the ridge; the two rows of mulberry trees are arranged in a staggered manner with row spacing of 60 cm and plant spacingwithin each row of 30 cm; and a drip irrigation device is arranged on the upper part of the ridge. The inventor employs the method in an experimental area in Yanbian County, Sichuan Province, and theplanting pattern reaches a leaf yield of 2800 kg per mu or more; with a traditional cultivation mode as a control, a mulberry fruit yield is increased by 30% to 120%, and no significant change occursin a mulberry leaf yield; the fruit yield is increased by 0.8 to 1.1 times, soluble solid content is as high as 24 to 29.25%, and the control is only 18 to 20%.

The Chinese medicine extract for curing hepatitis B contains the following active components (wt%):quercetin 0.1-5, isorhamnetin 0.1-20, total chlorogenic acid 0.1-50 and baicalin 1-60. Said invention also discloses the preparation method of said Chinese medicine extract. Said invented product not only possesses the action of resisitng hepatitis virus, but also can have the action of protecting liver and reducing transaminase. Its therapeutic effect is reliable, and it has no toxic side effect.

The invention discloses a vegetable cultivation nutrient bed and a vegetable cultivation method, and belongs to the technical field of vegetable soilless cultivation production. The vegetable cultivation nutrient bed mainly comprises surrounding walls on the two sides and a bed body in the middle, the bed body is composed of a water-retention film layer, a cobblestone layer, a water-permeable film layer and nutrient soil, and the water-retention film layer, the cobblestone layer, the water-permeable film layer and the nutrient soil are sequentially arranged from bottom to top. The water-retention film layer is a right-angle U-shaped plastic film located at the bottommost layer, and the nutrient soil is formed by uniformly mixing and fermenting the raw materials comprising, by weight, 5-15 parts of river sand, 25-35 parts of chaff, 25-35 parts of biogas slurry and 25-30 parts of sawdust. Vegetables cultured through the bed and the method are high in quality, high in yield, environmentally friendly, clean and healthy, save cost and have the advantages of being capable of maturing early, little in disease, free of inorganic fertilizers and free of pollution, saving water and labor and the like.

The invention relates to a medicament for preventing and treating atherosclerosis and a preparation method of a granular formulation, which are applied to the technical field of medicine. The medicament comprises the components of astragalus mongholicus, hawthorn, polygonum multiflorum, semen cassia, rhizoma alismatis, seaweed, ligusticum wallichii and angelica. The preparation method of the granular formulation comprises the following steps of: adding water to distill ligusticum wallichii and angelica; keeping distillate and decoction; heating and refluxing astragalus mongholicus, polygonum multiflorum and semen cassia twice by using ethanol; filtering; decompressing a filtrate; and recycling ethanol to obtain an ethanol extract of the medicament; adding water to decoct hawthorn, rhizoma alismatis, seaweed, astragalus mongholicus, polygonum multiflorum and semen cassia together; finely filtering; concentrating by using a triple-effect energy-saving concentrator to a relative density; drying a concentrated solution by using a spray drying method to prepare extractum powder; granulating; checking; and sub-packaging in an aluminum plastic film bag to obtain a finished product. The medicament provided by the invention has the advantages of simplicity in components, clear compatibility, less medicament taste, low price, and remarkable curative effect; and animal experiments show that the medicament has the effects of regulating blood lipid, protecting vascular endothelial cells, inhibiting expression of inflammatory factors and remarkably preventing and treating atherosclerosis.

The invention relates to the field of vegetable farming and discloses a farming method of vegetables. The farming method comprises the steps of laying fermented nutrient soil uniformly on a permeable membrane layer, with sufficient thickness according to the actual need of the vegetable roots of specific varieties; forming a drip irrigation hole in a position, close to each plant, of a drip irrigation pipe; sowing vegetable seeds or planting vegetable seedlings in the laid nutrient soil, and supplying water and nutrient solutions through drip irrigation; removing the original crop, uniformly sprinkling quicklime on the surface of the nutrient soil according to the usage amount of 100-120g / m<2>, performing digging and stirring to uniformly mix the nutrient soil with the quicklime, and then performing standing for 2-3 days. The farming method has the advantages of being high in vegetable quality and yield, saving in cost, green, environmentally friendly, clean, healthy, precocious, and light in diseases, and having no need of inorganic fertilizers, no pollution and saving of water and labor, etc. The realization of agricultural modernization is facilitated.

The invention relates to the technical field of animal model building, and in particular to a mouse tuberculosis dormant infection model establishment method. An incubation period of a mouse tuberculosis dormant infection model built by the method provided by the invention can be controlled to be within 20 weeks. In addition, the model built by the method provided by the invention accords with natural dormant infection characteristics of human. The condition that in the early phase of incubation (within 8 weeks), the mouse tuberculosis dormant infection model can be used for quickly and effectively evaluating antituberculosis drugs and vaccines without the need of using relapse in a later period of incubation is discovered for the first time. In addition, a model preparation technology in the invention can shorten the incubation period, stabilization in the incubation period is realized, and relapse conditions are relatively coincident, thus facilitating the research on incubation and relapse mechanisms. In addition, any intervention factor is not used, and an infection process of the model is the same as the natural dormant infection process of human, thus facilitating the research on an immunologic mechanism in the early phase.

The invention discloses an avian influenza virus-like particle antigen, a vaccine, a preparation method of the avian influenza virus-like particle antigen and application of the avian influenza virus-like particle antigen or the vaccine. A strain of recombinant baculovirus based on tandem expression of HA, NA and M1 genes of an H7N9 subtype avian influenza virus transfects insect cells and performs self-assembly to form the avian influenza virus-like particle antigen; and the virus-like particle antigen prepared according to the invention is used for preparing the vaccine. After a chicken group is immunized by the vaccine prepared according to the invention, HI antibodies, neutralizing antibodies and IgY antibodies with the higher titer can be generated, 100-percent clinical protection onthe H7N9 subtype highly pathogenic avian influenza virus can be provided, and virus expelling can be effectively inhibited. The rely on the chicken embryos is avoided; the production cost is low; theperiod is short; and higher protection capability can be induced only by once low-dose immunization. A foundation is laid for industrial production of VLP vaccines.

The invention discloses a recombinant H7N9 subtype avian influenza virus-like particle and its preparation method and application. Based on the recombinant baculovirus insect cell culture system, the expression of H7N9 highly pathogenic avian influenza virus HA protein and NA protein is respectively constructed. , M1 protein recombinant baculovirus. Three strains of recombinant baculoviruses were co-infected in suspended insect cells, and H7N9 subtype avian influenza virus-like particles self-assembled in the cells could be harvested. After being concentrated and purified by ultrafiltration tubes and different gradient concentrations of sucrose, it is mixed with adjuvant and emulsified to prepare the vaccine. The prepared vaccine immunized chickens can induce the body to produce specific antibodies, and has the advantages of strong immunogenicity, good safety, high genetic stability and the like. After challenge with a lethal dose of highly pathogenic avian influenza virus of H7N9 subtype, it can provide complete clinical protection and significantly inhibit the shedding of chickens. The invention provides a new method for preventing H7N9 subtype avian influenza virus infection, and also lays a foundation for the development of new influenza virus vaccines.

The invention relates to a medicament for preventing and treating atherosclerosis and a preparation method of a granular formulation, which are applied to the technical field of medicine. The medicament comprises the components of astragalus mongholicus, hawthorn, polygonum multiflorum, semen cassia, rhizoma alismatis, seaweed, ligusticum wallichii and angelica. The preparation method of the granular formulation comprises the following steps of: adding water to distill ligusticum wallichii and angelica; keeping distillate and decoction; heating and refluxing astragalus mongholicus, polygonum multiflorum and semen cassia twice by using ethanol; filtering; decompressing a filtrate; and recycling ethanol to obtain an ethanol extract of the medicament; adding water to decoct hawthorn, rhizoma alismatis, seaweed, astragalus mongholicus, polygonum multiflorum and semen cassia together; finely filtering; concentrating by using a triple-effect energy-saving concentrator to a relative density; drying a concentrated solution by using a spray drying method to prepare extractum powder; granulating; checking; and sub-packaging in an aluminum plastic film bag to obtain a finished product. The medicament provided by the invention has the advantages of simplicity in components, clear compatibility, less medicament taste, low price, and remarkable curative effect; and animal experiments show that the medicament has the effects of regulating blood lipid, protecting vascular endothelial cells, inhibiting expression of inflammatory factors and remarkably preventing and treating atherosclerosis.

The invention relates to a special fully-watersoluble organic fertilizer for flue-cured tobacco and belongs to the technical field of fertilizers. The special fully-watersoluble organic fertilizer is composed of, by weight, 28-32 parts of humic acid, 38-43 parts of water-soluble organic matters, 1-3 parts of organic calcium, 1.5-2.3 parts of organic magnesium, 0.8-1.2 parts of boron salt, 0.9-1.1 parts of copper salt, 0.85-1.2 parts of ferric salt, 0.7-1.1 parts of manganese salt, 0.9-1.3 parts of zinc salt, 7-10 parts of amino acid, 10-15 parts of fulvic acid and 0.9-1.3 parts of phosphorus pentoxide, wherein the boron salt, the copper salt, the ferric salt, the manganese salt and the zinc salt are all soluble in water. The special fully-watersoluble organic fertilizer is excellent in water solubility and less in impurities, water insoluble matters are smaller than or equal to 0.5%, and the fertilizer is quickly soluble in water and uniformly distributed into plant root soil along with water. Moreover, the special fully-watersoluble organic fertilizer is high in active ingredient content and small in usage amount, so that cost for packaging, storing, transporting and utilizing can be reduced.

The invention discloses a soil-improved organic planting method for flue-cured tobacco. The planting method includes: 1) selection of plots; 2) selection of flue-cured tobacco varieties; 3) soil improvement; 4) planting of flue-cured tobacco. The present invention achieves the purpose of effective improvement of deteriorated soil through comprehensive soil improvement methods of fallow, shallow plowing and deep plowing. The soil structure, beneficial microbial community, nutritional imbalance, etc. of the land are better improved. Tobacco plants grow well, and the yield and quality of tobacco leaves are significantly improved. The invention does not need chemical fertilizers and chemical pesticides in the whole process, adopts the field production and management mode, the whole growth period of the flue-cured tobacco produced is good in growth and development, and the tobacco leaves are delicate and disease-free.

The invention relates to the technical field of agricultural planting, in particular to a konjac double row strip planting and secondary ridge cultivation method. Konjac double-row row planting method for secondary ridge cultivation, comprising the following steps: A, site selection; B, soil disinfection; C, seed selection; D, planting taro disinfection; E, sowing; F, fertilization; G, cultivating soil moisture ; H, weeding; I, mulching; J, control of plant diseases and insect pests; K, excavation of konjac ball diameter. The invention has high seedling emergence rate, high fertilizer utilization rate, one-time fertilization, good drainage effect, good ventilation and light transmission, low disease occurrence rate, less labor input, high expansion rate of commodity taro bulbs, time-saving and labor-saving planting, and time-saving and excavation. Can be harvested mechanically.

A process for breeding a high-yield high-quality multi-resistance hybridized long-grained rice includes grouping the varieties IR24, Guangluai No.4 BG90-2 and Yanxian 3021 to selectively breed excellent line "3079" as male parent, choosing broad-affinity line "02428" as female parent, hybridizing, cyclic hybridizing, test cross to choose the recovery line "D206" and hybridizing between "D206" and sterile line.

The invention provides a vine guiding method for vine cowpea. According to the vine guiding method, vines of a row of vine cowpea are continuously guided with a sewing thread. The method specifically comprises steps as follows: a, a tightened steel wire is fixed over each row of vine cowpea; b, when vines of the vine cowpea begin to grow out, one end of each sewing thread is tied to the steel wire over the first vine cowpea plant in each row, then each sewing thread is guided to the base of the first vine cowpea plant in each row, winds around the stem of the first vine cowpea plant by one circle and is threaded between a vertical sewing thread and the plant and tensioned; c, each sewing thread is guided to the base of a second vine cowpea plant in each row in the horizontal direction, winds around the stem by one circle and is threaded from the position below the horizontal sewing thread and tensioned; e, each sewing thread is guided to the position over the third vine cowpea plant along the corresponding steel wire, winds around the steel wire by one circle and is threaded between the horizontal sewing thread and the steel wire and tensioned; f, each sewing thread is continuously guided to the last plant in each row with the method and tied to the steel wire or the base of the cowpea after being shorn off; g, vines of each row of the vine cowpea are guided with the same method. The method is simple, low in cost, good in commodity performance, economical and practical, and diseases are reduced.

The invention discloses a method for synchronously preventing and controlling chap and sunburn of persimmon fruits. The method comprises the following steps of (1) bagging at a proper period; (2) spraying chlorin iron on young fruits; (3) effectively applying a liquid fertilizer; (4) adjusting the microdomain ecology of a tree body; and after first physiological fruit drop of persimmon trees, spraying the chlorine iron with the mass ratio being 0.03 mg / kg and GA4 + 7 with the mass ratio being 10 mg / kg on a whole plant, particularly the surfaces of the young fruits, and spraying until water drops. According to the method for synchronously preventing and controlling dehiscence and sunburn of the persimmon fruits provided by the invention, through scientific application of comprehensive technical measures, the proportion of fruit epidermis and cross ditch dehiscence and fruit sunburn is remarkably reduced, harm of persimmon fruit moths and wasps is effectively avoided, and the appearance quality of the persimmon fruits is improved. Meanwhile, the inherent quality of the persimmon fruits is improved, the average single fruit quality of the fruits is improved by 7.85 percent compared with a control group, and the soluble solid content of pulp is improved by 4.94 percent compared with the control group.

The invention discloses an andrographis paniculata plug seedling cultivation method through sand culture. The andrographis paniculata plug seedling cultivation method comprises the following steps of: sowing andrographis paniculata seeds which are soaked for pregermination in a matrix of seedling cultivation plug, then pressing the seedling cultivation plug into a seedling cultivation tank which is filled with river sand and is watertight at bottom and the periphery, and irrigating to maintain sand bed to be humid so that the seeds absorb water for germination and growing; and when 8-10 pieces of euphylla grow out, transplanting the seedlings with the matrix into a large field. Compared with a conventional andrographis paniculata seedling cultivation technique, the andrographis paniculata plug seedling cultivation method has the advantages of low cost, less sowing quantity, convenience in management, saved labor and fertilizer and the like. Compared with a conventional seedling cultivation technique through nutritional soil, the cultivated seedlings have the advantages of good uniformity, robust plants, developed root systems, transplanting with the matrix, fast reviving and the like. Compared with a floating seedling cultivation technique, the andrographis paniculata plug seedling cultivation method has the advantages of convenience and uniformity in fertilizer and water management, and the cultivated seedlings have the advantages of unserious diseases, developed root systems and the like. The andrographis paniculata plug seedling cultivation method is beneficial to strong andrographis paniculata seedling cultivation and industrial production of the seedlings, thus providing effective technical support for andrographis paniculata GAP (Good Agricultural Practices) planting.

The invention discloses a method for efficient cultivation of brassica campestris in summer. The method comprises the following steps: S1, variety selection; S2, fertilization and bedding; S3, seed soaking with chlormequat chloride; S4, sowing; and S5, field management. Brassica campestris seeds are soaked with a mixed solution of a 50% chlormequat chloride aqueous solution with a concentration of12 mg / L, potassium dihydrogen phosphate with a concentration of 3 g / L and 50% amobam with a concentration of 5 g / L, the brassica campestris germinates quickly and neatly under a high temperature condition and has light diseases, and the yield is increased by about 20.0%. The method is simple, convenient, labor-saving and time-saving, and effectively improves the production benefits of the brassica campestris.