2 results about "H5N1 virus" patented technology

This invention discloses the preparation and application of a universal mRNA vaccine for H5N1 influenza. The mRNA vaccine contains mRNA molecule 1 and mRNA molecule 2; mRNA molecule 1 encodes the H5m protein; mRNA molecule 2 encodes the N1m protein; the H5m protein is the protein shown in SEQ ID NO:1; the N1m protein is the protein shown in SEQ ID NO:2. The universal mRNA vaccine for H5N1 influenza prepared by this invention possesses broad-spectrum protection, rapid iteration, and low-cost mass production characteristics. It can efficiently express native conformational proteins in host cells, not only covering the main variation spectrum of the H5N1 virus, but also overcoming the broad-spectrum limitations of traditional vaccines by activating cross-neutralizing antibodies and polyclonal T-cell immunity. This invention provides key technical support for addressing the continuous evolution and potential pandemic of H5N1.

The present application relates to the technical field of rapid detection of H5N1 avian influenza virus, and particularly relates to a nucleic acid releasing agent for H5N1 virus, and a preparation method and application thereof.The nucleic acid releasing agent is composed of the following components with a mass ratio of (50-80):1:(3.9-12.5):(0.02-0.25):(15-63):(12-20):(10-87): guanidine hydrochloride solution, TritonX-100, isopropyl alcohol, Tween-20, ethanol, EDTA and DEPC water.The nucleic acid releasing agent provided by the present application can efficiently release viral nucleic acid and remove interfering substances, and lays a foundation for detecting samples with low viral load;then, in combination with primer screening, reaction system and freeze-drying condition optimization, a visual freeze-drying RT-RAA detection system is constructed, and finally, rapid, sensitive, specific and visual detection of H5N1 virus is realized.