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Tobacco glutathione transferase gene and application thereof

A technology of glutathione and transferase, applied in transferase, application, genetic engineering and other directions, can solve problems such as economic loss of tobacco farmers and affecting tobacco production

Inactive Publication Date: 2015-11-11
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tobacco is an important economic crop in my country and occupies an important position in the national economy, but in its production practice, factors such as biological diseases and weeds seriously affect the production of tobacco, causing huge economic losses to tobacco farmers

Method used

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  • Tobacco glutathione transferase gene and application thereof
  • Tobacco glutathione transferase gene and application thereof
  • Tobacco glutathione transferase gene and application thereof

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Embodiment Construction

[0015] The protein encoded by the tobacco glutathione transferase gene NtGSTU17 has high CDNB coupling activity, and it is generally believed that high CDNB coupling activity means high herbicide resistance and detoxification activity. When tobacco seedlings were treated with different pesticides, tobacco glutathione transferase gene NtGSTU17 showed different expression characteristics, indicating that tobacco had different resistance and detoxification efficiency to different pesticides. In view of the detoxification activity of the protein expressed by this gene, it may play an important role in the detoxification metabolic reaction, and has certain application value and application prospect in screening low-toxicity, easy-to-remove pesticides and pesticide safeners. be protected.

[0016] The gene provided by the present invention is derived from tobacco (Nicotianatabacum), named NtGSTU17, and encodes a tobacco glutathione transferase protein, which is the protein of (i) or...

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Abstract

The invention discloses tobacco glutathione transferase protein, the effect of a tobacco glutathione transferase gene NtGSTU17 in detoxication metabolism, and application of the tobacco glutathione transferase gene in tobacco pesticide safener screening, and belongs to the technical field of biology. The amino acid sequence of the tobacco glutathione transferase gene can be seen in SEQ ID NO:1. Glutathione transferase (GST) is a key enzyme generally existing in the organism detoxication metabolism process, the tobacco NtGSTU17 protein has high detoxication activity, the encoding gene of the tobacco NtGSTU17 protein is expressed in tobacco in a constitutive mode and is remarkably expressed in an up regulation mode after being processed through some pesticide, and it shows that the gene has application value and economic value on the aspects of tobacco pesticide detoxication and screening.

Description

technical field [0001] The invention relates to the application of tobacco glutathione transferase gene NtGSTU17, a key gene of tobacco detoxification metabolic pathway, in the detoxification of tobacco pesticides and the screening of pesticide safeners. Background technique [0002] Glutathione Transferase (GSTs) is one of the most abundant proteases in nature. GSTs have a variety of catalytic activities: reduced glutathione (GSH) can be covalently coupled to non-polar compounds containing electrophilic carbon, nitrogen, and sulfur atoms, so that GSTs can be used as central components to participate in drugs, Detoxification and metabolism of pesticides and other heterogeneous substances; GSTs can also catalyze the reduction of some metabolites of oxidative stress, such as peroxides, so that GSTs play an important role in the defense against bacterial infection and abiotic stress. The research on glutathione transferase in plants began with Fear's research on the herbicide-...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12Q1/68
CPCC12N9/1088C12Q1/6895C12Y205/01018
Inventor 周会娜翟妞陈千思刘萍萍陈霞毛李鸿郑庆霞金立锋林福呈
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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