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The application discloses a dCAPS molecular marker for amplifying a SNP site of a QTL of wheat scab and application thereof in the technical field of molecular biology, is located on the long arm of chromosome 5B of a wheat Chinese spring reference sequence, and a primer pair comprises a forward primer and a reverse primer, and the nucleotide sequences of the forward primer and the reverse primer are shown as SEQ ID NO. 1-2. The molecular marker is closely linked to scab resistance in the application, has high diagnostic accuracy, and can be widely used in assisted breeding of wheat resistant to scab.

The application provides a method for predicting overwintering rate and cold resistance of Brassica napus, and belongs to the technical field of rape cultivation. The method comprises the following steps: sowing seeds of Brassica napus to be evaluated in a field; in early December, after the minimum temperature in the field is lower than-4 DEG C and lasts for 5-10 days, the key indicators of Brassica napus are determined; the determined key indicators are substituted into a regression equation of a linear regression model to calculate the predicted overwintering rate of Brassica napus; and the cold resistance of Brassica napus is identified according to the obtained predicted overwintering rate. Compared with the traditional natural overwintering identification, the application can obtain the predicted overwintering rate by only determining 7 key indicators once before winter, and complete the cold resistance grading evaluation, so that the identification period is shortened.