Leading stabilizing element for enhancing activity and expression of exogenous protein in bacteria
A technology for exogenous proteins and stabilizing elements, applied in the biological field, can solve the problems of lack of activity, harmful effects of heterologous proteins, and protein instability, and achieve the effects of enhancing expression and activity, and improving protein yield and protein activity.
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[0027] A leading stable element that enhances the activity and expression of exogenous proteins in bacteria is a 208-amino acid residue fragment of the N-terminal of the Salmonella enterica subsp. Shown in SEQ.ID.NO:1, the amino acid sequence is shown in SEQ.ID.NO:2.
[0028] Identification of lead stabilizing elements that enhance the activity and expression of foreign proteins in bacteria:
[0029] 1. Cloning the minimal promoter (SEQ.ID.NO: 3) of the Salmonella enterica subsp. enterica Chlamydia typhimurium strain RM13672 SspH1 and the coding sequence of 208 amino acid residues at the N-terminal of the SspH1 protein (a total of 889 nucleotide sequences, see figure 1 shown);
[0030] According to the SspH1 gene coding region design primer pair pSspH1-265-F / pSspH1-R2 in the chromosome sequence of Salmonella enterica subsp. pSspH1-R2, bacterial genomic DNA and high-fidelity Tag DNA polymerase to establish polymerase chain reaction (PCR), the reaction conditions are 95°C for ...
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