Ruthenium-based metal ion liquid polymer microsphere artificial enzyme as well as preparation method and application thereof

An ionic liquid and metal-based technology, which is applied in the field of ruthenium-based metal ionic liquid polymer microsphere artificial enzyme and its preparation, can solve the problem of low activity, achieve high catalytic activity, high oxidase-like activity, and good reproducibility Effect

Active Publication Date: 2022-04-05
SPECIAL EQUIP SAFETY SUPERVISION INSPECTION INST OF JIANGSU PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the activity of existing artificial enzymes is significantly lower than that of natural enzymes

Method used

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  • Ruthenium-based metal ion liquid polymer microsphere artificial enzyme as well as preparation method and application thereof
  • Ruthenium-based metal ion liquid polymer microsphere artificial enzyme as well as preparation method and application thereof
  • Ruthenium-based metal ion liquid polymer microsphere artificial enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of ruthenium-based metal ionic liquid polymer microsphere artificial enzyme, its preparation method comprises the steps:

[0038](1) Mix 60mL of salicylic acid (0.1mol / L), 60mL of vinylimidazole (0.1mol / L) and 10mL of ruthenium trichloride (0.1mol / L) in water, stir at 65°C for 60min at 60r / min , to obtain a black mixture. Then extract with dichloromethane, remove the solvent by rotary evaporation of the oil phase solution, the temperature of the rotary evaporation is 70°C, and the time is 1h to completely remove the solvent, and the resulting solution is vacuum-dried overnight at 75°C to obtain a black oily liquid that is ruthenium base Vinylimidazole Salicylate Ionic Liquid.

[0039] (2) Take 10g of ionic liquid and disperse it in 100mL of water, add 100g of divinylbenzene, and use a homogenizer to mix and homogenize. Based initiator, reflux reaction in a nitrogen atmosphere for 24h, the reaction temperature is 100°C, then filtered and washed 5 times with deio...

Embodiment 2

[0045] A kind of ruthenium-based metal ionic liquid polymer microsphere artificial enzyme, its preparation method comprises the steps:

[0046] (1) Mix 60mL of salicylic acid (1.2mol / L), 50mL of vinylimidazole (1.2mol / L) and 15mL of ruthenium trichloride (1.2mol / L) in water, stir at 65°C for 45min at 75r / min , to obtain a black mixture. Then extract with dichloromethane, remove the solvent by rotary evaporation of the oil phase solution, the temperature of the rotary evaporation is 70°C, and the time is 1h to completely remove the solvent, and the resulting solution is vacuum-dried at 70°C for 8h to obtain a black oily liquid that is ruthenium base Vinylimidazole Salicylate Ionic Liquid.

[0047] (2) Take 100g of ionic liquid and disperse it in 100mL of water, add 100g of divinylbenzene, and use a homogenizer to mix and homogenize. Based initiator, reflux reaction in a nitrogen atmosphere for 10h, the reaction temperature is 60°C, then filtered and rinsed with deionized wate...

Embodiment 3

[0050] A kind of ruthenium-based metal ionic liquid polymer microsphere artificial enzyme, its preparation method comprises the steps:

[0051] (1) Mix 60mL salicylic acid (0.05mol / L), 60mL vinylimidazole (0.05mol / L) and 10mL ruthenium trichloride (0.05mol / L) aqueous solution, stir at 65°C for 55min at 60r / min , to obtain a black mixture. Then extract with dichloromethane, remove the solvent by rotary evaporation of the oil phase solution, the temperature of the rotary evaporation is 70°C, and the time is 1h to completely remove the solvent, and the resulting solution is vacuum-dried at 100°C for 12h to obtain a black oily liquid that is ruthenium base Vinylimidazole Salicylate Ionic Liquid.

[0052] (2) Take 20g of ionic liquid and disperse it in 100mL of water, add 100g of divinylbenzene, use a homogenizer to mix and homogenize, the speed of homogenization is 900r / min, the time is 5min, after mixing evenly, add 0.066g of ammonium persulfate free Based initiator, reflux rea...

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Abstract

The invention discloses a ruthenium-based metal ion liquid polymer microsphere artificial enzyme as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing a salicylic acid solution, a vinyl imidazole solution and a ruthenium trichloride solution, stirring, extracting, evaporating the obtained oil phase solution to remove a solvent, and drying to obtain ruthenium salicylic acid vinyl imidazole ionic liquid; (2) dispersing the ionic liquid obtained in the step (1) in a solvent, adding divinyl benzene, mixing, adding a free radical initiator, reacting, separating, precipitating, washing with water, and drying to obtain solid powder; and (3) dispersing the solid powder obtained in the step (2) in a solvent to obtain a reactant, adding a reducing agent to react, and drying the solid obtained by filtering to obtain the ruthenium-based metal ion liquid polymer microsphere artificial enzyme. The artificial enzyme prepared by the invention has good dispersibility and high oxidase-like activity, and can be used for detecting prochloraz by a colorimetric method.

Description

technical field [0001] The invention relates to the field of preparation and application of artificial enzymes, in particular to a ruthenium-based metal ion liquid polymer microsphere artificial enzyme and its preparation method and application. Background technique [0002] Strawberry fruit is bright red and beautiful, soft and juicy, sweet and sour, fragrant and rich in nutritional value. It is known as the "Queen of Fruits" and is rich in vitamin C, vitamin A, vitamin E, vitamin PP, vitamin B1, vitamin B2, Nutrients such as carotene, tannic acid, aspartic acid, copper, strawberry amine, pectin, cellulose, folic acid, iron, calcium, ellagic acid and anthocyanins. Strawberry is native to South America and was introduced to my country in the early 20th century. In recent years, the planting area has continued to expand, and it has become one of the largest strawberry producing countries in the world, and the planting area has maintained a market trend of continuous growth. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02B01J13/14C08F212/36C08F230/04G01N21/31G01N21/78
CPCY02P20/54
Inventor 杨永强王勤生李在均王群丁杰
Owner SPECIAL EQUIP SAFETY SUPERVISION INSPECTION INST OF JIANGSU PROVINCE
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