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Platelet aggregation function detection kit and detection method

A technology for platelet aggregation and function detection, applied in the field of platelet detection, can solve the problems of low accuracy and stability, large variation in repeated detection, long sample processing time, etc. Handling less demanding effects

Inactive Publication Date: 2013-03-20
北京乐普诊断科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its main disadvantages include: the blood sample processing needs to be completed in a short time, the repeated detection has a large variation, the blood collection volume is relatively large, and the platelet-rich plasma needs to be separated, so the sample processing time is long, etc.
The disadvantages of this technology are: low accuracy and stability

Method used

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  • Platelet aggregation function detection kit and detection method
  • Platelet aggregation function detection kit and detection method
  • Platelet aggregation function detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1. Preparation of AA Activation Pathway Platelet Aggregation Function Detection Kit

[0060] Sodium citrate whole blood activator: Kaolin is prepared with normal saline to 0.12g / L, and each cup is divided into 10μL; CaCl 2 Prepare 0.2mol / L with normal saline, and dispense 20μL per cup. The above components were freeze-dried and stored at the bottom of a colorless measuring cup.

[0061] Fibrin activator: Recombinant batroxobin is prepared with pure water at 1 mg / mL, and each cup is filled with 5 μL; activated blood coagulation factor XIIIa is used, prepared with pure water at 1 mg / mL, and each cup is filled with 3.5 μL; enzyme stabilizer Select bovine serum albumin (BSA), prepare 20% (w / v) with pure water, and fill 10 μL per cup; use lactose as plastic agent, prepare 20% (w / v) with pure water, divide Contain 10 μL; prepare sodium azide with pure water to 3% (w / v), and fill each cup with 8 μL. The above components were freeze-dried and stored at the bottom of the blue...

Embodiment 2

[0083] 1. Preparation of ADP Activation Pathway Platelet Aggregation Function Detection Kit

[0084] Others are the same as in Example 1, the difference is that the platelet activator is ADP, prepared as a 0.2mmol / L solution with physiological saline, divided into 10 μL per cup, freeze-dried together with the fibrin activator and stored at the bottom of the green measuring cup .

[0085] 2. Use the above detection kit to detect the inhibition rate of clopidogrel (or other thienopyridine drugs) on the ADP membrane receptor channel

[0086] Detection steps:

[0087] (1) Collect sodium citrate anticoagulated whole blood and heparin anticoagulated whole blood samples from the same patient taking clopidogrel;

[0088] (2) Determination of the clot strength MA of sodium citrate anticoagulated whole blood with a colorless measuring cup 总 ;

[0089] (3) Measure the clot strength MA of heparin anticoagulated whole blood with a blue measuring cup 纤 ;

[0090] (4) Measure clot stre...

Embodiment 3

[0104] Detection of Abciximab's Inhibitory Rate of GPIIa-IIIb Membrane Receptor Channel Using TRAP as Activator

[0105] Others are the same as in Example 1, except that the platelet activator is TRAP, and the detected activation pathway is GPIIa-IIIb membrane receptor channel. The measured inhibition rate was 48.2%, and the inhibition effect was poor.

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Abstract

The present invention relates to a platelet aggregation function detection kit, and a method using the kit to detect platelet aggregation function. The detection kit mainly includes the following detection reagents: a sodium citrate whole blood activator, a fibrin activator and a platelet activator. The fibrin activator comprises: recombinant batroxobin and activated clotting factor. By using the kit of the invention to detect, blood sample processing requirements are low, repeat testing variability is small, accuracy and stability are high, use and storage are convenient, detection cost is low, and promotion is easy.

Description

technical field [0001] The invention relates to the field of platelet detection, in particular to a platelet aggregation function detection kit and a method for detecting platelet aggregation function using the kit. Background technique [0002] Platelets play an important role in physiological hemostasis, maintaining the integrity of blood vessel walls, and certain pathological processes, such as thrombosis, atherosclerosis, unstable angina, tumor metastasis, and inflammatory reactions. A large number of studies have shown that the activation and aggregation of platelets is the initiating factor of thrombus formation in vivo, and it is also one of the most critical components in the process of thrombus formation. Common clinical critical emergencies such as sudden cardiac death, myocardial infarction, stroke, and pulmonary infarction are all thrombotic diseases. Therefore, platelet function testing is of great significance for the early detection of thrombosis risk and the...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/15
Inventor 周高英
Owner 北京乐普诊断科技股份有限公司
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