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Hemostasis composition containing batroxobin and preparation method thereof

A thrombin-like and composition technology, applied in the field of hemostatic compositions and their preparation, can solve the problems of inability to cross-link fibrin, carrying zoonotic viruses and the like

Inactive Publication Date: 2010-08-11
BEIJING SAISHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

4. It has no activation effect on coagulation factor XⅢ, and cannot cross-link fibrin
Some people use porcine thrombin, but there is also the possibility of carrying zoonotic viruses

Method used

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  • Hemostasis composition containing batroxobin and preparation method thereof
  • Hemostasis composition containing batroxobin and preparation method thereof
  • Hemostasis composition containing batroxobin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A. Preparation of fibrinogen and its buffer solution: Melt and cool porcine whole plasma at 37°C to below 10°C, slowly add 50% ethanol at a volume percentage of pre-cooled -20°C and keep stirring until the final concentration is 10% ethanol, as 12 hours of precipitation treatment, centrifuged at 5000 rpm for 30 minutes. The precipitate was then dissolved in neutral Tri / citrate-lysine buffer solution with a final protein concentration of about 1.5% (weight percent). Then pre-cooled 50% ethanol for 10-12 hours at 4°C for the second 10% ethanol precipitation. Centrifuge at 5000 rpm for 30 minutes to dissolve the precipitate to 40mg / ml, then sterilize and filter, and divide into 5ml bottles for lyophilization. The fibrinogen solution is a buffer solution containing 0.02M pH7.4 sodium citrate.

[0054] Preparation of type B thrombin and its buffer: Weigh 10 g of Agkistrodon halys venom (purchased from Liaoning Qingyuan Agkistrodon breeding factory), extract with 0.02mol / L ...

Embodiment 2

[0059] The fibrinogen and thrombin-like lyophilized powder obtained according to the method in Example 1 were mixed according to the ratio of 100 mg of fibrinogen and 40 international units (IU) of thrombin-like, added absolute ethanol and stirred evenly, and then the collagen sponge One side is in contact with the suspension and dried in vacuum.

Embodiment 3

[0061] With the fibrinogen and thrombin-like lyophilized powder obtained according to the method in Example 1, 200 mg of fibrinogen and 40 international units (IU) of thrombin-like per capsule, and 20 mg of omeprazole and appropriate amount of auxiliary materials were added, Capsules are prepared.

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Abstract

The invention provides a novel hemostasis composition which comprises fibrinogen or hemaleucin, and batroxobin, wherein the ratio of the batroxobin to the fibrinogen or hemaleucin is 0.01:100IU:1mg. Because a clot formed by the batroxobin can not cause contraction, and the batroxobin can not cause crossimmunity reaction, the hemostasis composition can overcome multiple defects existing in hemostasis products containing thrombin or prothrombin.

Description

technical field [0001] The invention relates to a hemostatic composition and a preparation method thereof, more particularly to a hemostatic composition containing thrombin-like enzyme and a preparation method thereof. Background technique [0002] The coagulation process of humans and animals includes the formation and cross-linking of fibrin, which includes the formation of fibrin monomers, polymerization and cross-linking of fibrin. [0003] 1) Formation of fibrin monomer: Fibrinogen is synthesized by the liver and has two α chains (Aa), two β chains (Bβ), and two γ chains (γ2), namely three pairs of different polypeptide chains The constituent glycoproteins can be represented by (Aa, Bβ, γ)2. Under the action of thrombin, fibrinogen releases A and B small peptides to form fibrin monomers. [0004] 2) Polymerization and cross-linking of fibrin monomers: Polymer clots formed by connecting soluble fibrin monomers through secondary bonds such as hydrogen bonds. Although blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61P7/04A61K38/36A61K38/18A61K33/06
Inventor 马骉孔双泉宋梦薇孙爽
Owner BEIJING SAISHENG PHARMA
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