Construction method of diabetic cardiomyopathy animal model

Abstract

The invention discloses a construction method of a diabetic cardiomyopathy animal model. An SD (Sprague Dawley) rat under high-sugar and high-fat feeding is subjected to multiple abdominal injection with STZ (streptozocin); after the diabetics diagnosis standard is reached, subcutaneous injection with isoprenaline is performed; after 2 weeks of continued feeding, left ventricular cannulation and three-lead electrocardiogram test of ventricular hemodynamic parameters and electrocardiogram parameters shows that the rat experiences cardiac dysfunction; heart weight index and left ventricular index show that the rat experiences evident ventricular hypertrophy; biochemical analysis on rat blood shows that the rat has evident myocardial damage; HE (hematoxylin-eosin) pathological staining satisfies pathological changes in diabetic cardiomyopathy; Masson collagen staining satisfies myocardial connective tissue proliferation of diabetic cardiomyopathy. The construction method has the advantages of good operational simplicity, short construction time, low mortality in construction phase, high construction success rate and the like, and the pathological process of myocardial damage due to human type 2 diabetes mellitus can be well simulated.

Description

technical field [0001] The invention belongs to the field of pharmacology, and in particular relates to a method for making a rat type II diabetic cardiomyopathy model. Background technique [0002] Diabetes mellitus (DM) is a metabolic disease caused by insufficient insulin secretion or peripheral tissue insensitivity to insulin, characterized by persistent hyperglycemia, and may cause various tissues and organs (such as eyes, kidneys, , heart, blood vessels, nerves, etc.) long-term damage, insufficiency or failure, most of which are type 2 diabetes. According to statistics, in 2015, about 415 million people aged 20 to 79 in the world suffered from diabetes (8.8% prevalence rate), and another 318 million people had impaired glucose tolerance (pre-prevalence rate was 6.7%). China is the country with the largest number of diabetic patients in the world. In 2015, the number of patients reached 109.6 million, and 1.3 million people died of diabetes and its complications. At t...

AI Technical Summary

Problems solved by technology

At present, there are few research models of diabetic cardiomyopathy, only reports of the hyperglycemia state induced by injection of streptozotocin (STZ) to establish a spontaneous model, but the modeling time is long (especially type Ⅱ diabetic cardiomyopathy), animal The mortality rate is high (diabetic cardiomyopathy itself is one of the reasons), the modeling rate of the model is low, and the degree of pathological changes varies greatly, which cannot fully reflect the chronic pathological and physiological changes in the occurrence and development of diabetic cardiomyopathy

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