LC-MS/MS (Liquid Chromatography-Mass Spectrometry/Mass Spectrometry) determination method of aloesin in rat plasma

A kind of aloe verain and determination method technology, applied in the field of aloe verain, can solve the problems of backward analysis technology, less aloe verain, no pharmacokinetic parameters provided, etc., and achieve the effect of accurate detection
CN112415116AActive Publication Date: 2021-02-26HAINAN MEDICAL COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HAINAN MEDICAL COLLEGE
Publication Date
2021-02-26

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Abstract

The invention provides an LCMS / MS determination method of aloesin in rat blood plasma, which comprises the following steps: (1) blood plasma sample treatment: taking blood plasma to be detected containing aloesin, adding a methanol solution containing internal standard aloin D, carrying out vortex precipitation, centrifuging the mixture, and collecting the supernatant as a sample to be detected; (2) setting liquid chromatography conditions as follows: a mobile phase A and a mobile phase B are adopted for gradient elution, the mobile phase A is an aqueous solution containing 0.01-1.0% o (v / v) of formic acid, and the mobile phase B is methanol; and (3) setting mass spectrum conditions as follows: an electrospray ion source, negative ion detection, spray voltage of 4500V, spray temperature of550 DEG C, a multi-reaction ion monitoring scanning mode, an ion pair for quantitative analysis of aloesin of m / z 393.1 -> 272.9, and an internal standard compound aloin D ion pair of m / z 555.3 -> 144.9. The method provided by the invention can be used for detecting aloesin in blood plasma more quickly, sensitively and accurately.
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Description

technical field

[0001] The invention relates to the technical field of aloesin, in particular to an LC-MS / MS determination method for aloesin in rat plasma. Background technique

[0002] Aloepicrin is a kind of chromone glycosides, which exists in various plants of the genus Aloe in Liliaceae, and the content in Aloe barbadensis Miller is about 3‰. Aloe vera is a traditional Chinese medicine with a long history of application. The juice of its leaves is concentrated and dried before being used as medicine. Relevant studies have shown that aloesin is one of the important active substances of aloe. Aloesin can inhibit the activity of tyrosinase, prevent the synthesis of melanin, and has whitening effect. Aloesin can regulate the release of inflammatory factors and growth factors in cells, produce anti-inflammatory effects; induce the activation of Smad and MAPK signaling proteins, and promote wound healing. Aloepicrin can also activate the WNT signaling pathway, down-regula...

Claims

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