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Method for simultaneously detecting paracetamol and its eight blood plasma metabolites

A technology for paracetamol and metabolites, which is applied in the field of drug analysis and can solve the problems of incomplete metabolites and inability to achieve quantitative detection.

Active Publication Date: 2017-12-19
华润三九(枣庄)药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, on the one hand, the above-mentioned method can only detect five plasma metabolites of acetaminophen, and the metabolites of quantitative detection are not comprehensive; on the other hand, the quantitative lower limit of acetaminophen and its five plasma metabolites in the above method All at the μg / ml level, quantitative detection of the five plasma metabolites at lower concentrations cannot be achieved

Method used

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  • Method for simultaneously detecting paracetamol and its eight blood plasma metabolites
  • Method for simultaneously detecting paracetamol and its eight blood plasma metabolites
  • Method for simultaneously detecting paracetamol and its eight blood plasma metabolites

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Effect test

Embodiment 1

[0064] In this embodiment, the method for simultaneously detecting acetaminophen and its eight plasma metabolites comprises the following steps:

[0065] (1) Configuration of internal standard reference substance solution

[0066] Precisely weigh phenacetin PNT and oreoside OA, add appropriate amount of methanol respectively to obtain stock solutions of phenacetin PNT and oreoside OA with a concentration of 1 mg / mL, and store them at -80°C for later use;

[0067] Accurately measure 10 μL of oreoside OA stock solution, place it in a 2 mL centrifuge tube, add 990 μL of methanol, and obtain an oreoside OA solution with a concentration of 10 μg / mL;

[0068] Take 10 μg / mL of oreoside OA solution and 10 μL of phenacetin PNT stock solution with a concentration of 1 mg / mL respectively, add them to a 2 mL centrifuge tube, add 980 μL of methanol, and obtain a concentration of 100 ng / mL. The reference substance solution of pteroside and phenacetin with a concentration of 10 μg / mL is sto...

Embodiment 2

[0082] In this embodiment, the method for simultaneously detecting acetaminophen and its eight plasma metabolites comprises the following steps:

[0083] (1) Configuration of internal standard reference substance solution

[0084] Precisely weigh phenacetin PNT and orogenin OA, add appropriate amount of methanol respectively to obtain stock solutions of phenacetin PNT and orogenin OA with a concentration of 0.9 mg / mL, and store them at -90°C for use;

[0085] Accurately measure 9 μL of oreoside OA stock solution, place it in a 1 mL centrifuge tube, add 990 μL of methanol to obtain an oreoside OA solution with a concentration of 9 μg / mL;

[0086] Take respectively 9 μL of the oleiferin OA solution with a concentration of 9 μg / mL and the stock solution of phenacetin PNT with a concentration of 0.9 mg / mL, add them to a 1 mL centrifuge tube, add 980 μL of methanol, and obtain a concentration of 90 ng / mL The reference substance solution of oreoside and phenacetin with a concentrat...

Embodiment 3

[0101] In this embodiment, the method for simultaneously detecting acetaminophen and its eight plasma metabolites comprises the following steps:

[0102] (1) Configuration of internal standard reference substance solution

[0103] Precisely weigh phenacetin PNT and oreoside OA, add appropriate amount of methanol respectively to obtain stock solutions of phenacetin PNT and oreoside OA with a concentration of 1.1 mg / mL, and store them at -70°C for later use;

[0104] Accurately measure 11 μL of oreoside OA stock solution, place it in a 3 mL centrifuge tube, add 990 μL of methanol, and obtain an oreoside OA solution with a concentration of 11 μg / mL;

[0105] Take respectively 11 μL of the oreoside OA solution with a concentration of 11 μg / mL and 11 μL of the phenacetin PNT stock solution with a concentration of 1.1 mg / mL, add it to a 3 mL centrifuge tube, add 980 μL of methanol, and obtain a concentration of 110 ng / mL The reference substance solution of oreoside and phenacetin w...

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Abstract

The invention concretely relates to a method for simultaneously detecting paracetamol and its eight blood plasma metabolites. An experiment result shows that the method allows the lower quantification limit of the paracetamol and its eight blood plasma metabolites to reach 0.46 ng / mL, and the correlation coefficient of every linear regression equation is 0.998; the intra-day accuracy of all detected substances is 85-111%, and the precision is lower than 9%; and the inter-day accuracy of all the detected substances is 94-108%, and the precision is lower than 9%. The method is fast, sensitive and accurate, and is suitable for quantitatively detecting the paracetamol and its eight blood plasma metabolites in rat blood plasma.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method capable of simultaneously detecting acetaminophen and eight plasma metabolites thereof. Background technique [0002] Ganmaoling Granules (GML, containing APAP 200mg) is a compound preparation of Chinese and Western medicines that was launched in the late 1970s. It has antipyretic and analgesic effects and is used for headache, fever, nasal congestion, runny nose, sore throat and other symptoms caused by colds. Treatment. Acetaminophen (Acetaminophen, APAP), one of the main medicinal components of Ganmaoling Granules, is a commonly used over-the-counter antipyretic and analgesic drug in clinical practice, and its safety is relatively high in therapeutic doses. [0003] However, APAP content in some drugs is relatively high, and excessive APAP can cause acute liver injury and even cause death of the individual. Studies have shown that the metabolites of APAP are the main ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/34G01N30/06
CPCG01N30/02G01N30/06G01N30/34G01N30/72
Inventor 李鹰飞李川杨军令吴思玚张跃飞朱玉华
Owner 华润三九(枣庄)药业有限公司
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