Method for Screening Active Components, Synergistic and Antagonistic Components in Compound Panax notoginseng Using Rat Blood Stasis Model

A technology for promoting blood circulation and removing blood stasis and active ingredients, which is applied in the field of in vivo evaluation of the pharmacological activity of traditional Chinese medicines, can solve the problems of unclear composition and composition of drug-containing serum, inability to evaluate the efficacy of drugs, etc., and achieves objective and effective evaluation results of traditional Chinese medicines. The effect of technical bottlenecks

Active Publication Date: 2018-01-02
SHENYANG PHARMA UNIVERSITY
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Problems solved by technology

The main problems of traditional Chinese medicine serum pharmacological experiment methods are: ① The specific components and composition of the drug-containing serum used in the test are not clear;

Method used

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  • Method for Screening Active Components, Synergistic and Antagonistic Components in Compound Panax notoginseng Using Rat Blood Stasis Model
  • Method for Screening Active Components, Synergistic and Antagonistic Components in Compound Panax notoginseng Using Rat Blood Stasis Model
  • Method for Screening Active Components, Synergistic and Antagonistic Components in Compound Panax notoginseng Using Rat Blood Stasis Model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. After the blood stasis model rats were given compound Sanqi, the drug-containing serum extract was reinjected to evaluate its effect of promoting blood circulation and removing blood stasis

[0039] 1 Pharmacodynamic evaluation after administration of original traditional Chinese medicine

[0040] 1.1 Blood stasis model preparation

[0041] Male Wistar rats, weighing 240±20g, were randomly divided into 9 time groups ranging from 1.0 to 6.0 hours, with 12 rats in each group. Rats in each group were subcutaneously injected with epinephrine hydrochloride 0.8mg / kg (2ml / kg) twice with an interval of 4h. During the interval, the rats were placed in an ice bath at 0°C and swam for 5min to prepare a rat model of acute blood stasis.

[0042] 1.2 Pharmacodynamic evaluation of promoting blood circulation and removing blood stasis

[0043] Eighteen hours after modeling, the rats in each group were divided into two groups, and were injected with normal saline solution (...

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Abstract

The invention belongs to the technical field of medicine, and relates to a method for evaluating the pharmacological activity of traditional Chinese medicine in vivo. The specific technical scheme is: after administering the traditional Chinese medicine to experimental animals, evaluate the changing trend of pharmacological indicators, and collect and administer the medicine at the best time for the action of the traditional Chinese medicine. The biological fluids of animals are reinjected into the experimental animals after appropriate treatment, and then the change trend of the pharmacological indicators is evaluated and compared with the data obtained after direct administration of traditional Chinese medicine to evaluate the time-lag effect of traditional Chinese medicine on the pharmacological indicators. The invention combines the whole pharmacokinetics of the traditional Chinese medicine based on the modern analysis technology to evaluate the effective components and the synergistic or antagonistic components in the effective substances of the traditional Chinese medicine.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine pharmacology, in particular to a new method for evaluating the pharmacological activity of traditional Chinese medicine in vivo. Background technique [0002] The experimental pharmacological method of traditional Chinese medicine is an important part of the field of activity evaluation of traditional Chinese medicine, and the traditional method of experimental pharmacological evaluation of traditional Chinese medicine is in vitro test method. The in vitro test method has strong conditional controllability, and can conduct comprehensive evaluations of ultramicro, biochemical, receptor, gene and other aspects at the cellular and subcellular levels. However, the in vitro test method is the evaluation result obtained by directly adding the crude extract of traditional Chinese medicine into the reaction system for the test. In many cases, the evaluation result not only cannot be consistent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
Inventor 于治国赵云丽赵星陈晓辉
Owner SHENYANG PHARMA UNIVERSITY
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