81 results about "Aging cell" patented technology

The invention discloses a label-free cell detection device and method based on light sheet illumination. The method includes the steps that a light sheet generation unit is used for shaping a laser beam into a uniform illumination light sheet with the micron-order thickness through a cylinder lens; the light sheet excites particles or a cell suspension placed in a sample micro-cavity chamber controlled by a precise displacement table to move; a form microscopic image and a two-dimensional light scattering pattern of a single particle or cell are recorded by a detector under the focusing mode and the defocusing mode through an objective lens; and a result is input into an imaging analysis system for image processing and recognizing and classifying. By means of the light sheet illumination method, a stimulation area can be effectively limited, background interference in light scattering imaging is inhibited, and effective stimulation of the single particle or cell and particle size discrimination of the sub-micron resolution ratio level are realized; and a light sheet illumination stimulation two-dimensional light scattering technology can avoid complex dyeing operation and a fluorescence signal detection process, and label-free detection and classification are carried out on aging cells. The label-free cell detection device and method based on light sheet illumination are high in applicability and capable of being popularized.

The invention discloses a human-derived stem cell bioactive substance, its preparation and application. The method comprises: first extracting healthy human-derived stem cells, which are then subjected to in vitro culture and amplification, collecting stem cells of specific phases, and utilizing modern ultralow temperature as well as other biological technologies to prepare a stem cell bioactive substance group composition. Mainly coming from mesenchymal stem cells and hematopoietic stem cells, the stem cell substance group can be developed into products with a cytobiological activity function. When applied on human bodies, the products can activate body stem cells, repair or substitute damaged, pathological and aging cells, adjust the metabolic function of body cells, enhance body immunity, and delay aging, etc. The human-derived stem cell bioactive substance group composition in the invention boasts substantial application prospects in medicine, healthcare and beauty maintaining fields.

The invention discloses an original skin lotion. The original skin lotion is prepared from 0.015-0.025 wt% of protease, 0.01-0.03 wt% of protease dextran and the balance water. The whole original skinlotion formula abandons existing complex and multifunctional formulas, the concise formula fitting the original skin is embodied, hydrosolvent is matched with protease and protease dextran to condition the skin together, the cuticle is conditioned easily without greasy feeling, an amino acid derivative film is formed on the skin surface layer by protease, protease dextran improves skin water circulation activity, effective moisture preservation and water replenishing of the skin net structure are ensured, the dry skin is effectively moistened, the skin is kept wet and smooth, the original skin lotion helps to metabolize epidermis old cells, aging cell accumulation is reduced, skin metabolism is promoted, and the skin is activated.

The invention discloses a skin care product capable of enhancing skin resistance and metabolic power. The skin care product comprises, by weight, 4-6 parts of a grape seed extract, 2-3 parts of collagen, 1-2 parts of allantoin, 1-3 parts of hyaluronic acid, 10-12 parts of glycerinum, 120-140 parts of purified water, 1-3 parts of lavender essential oil, 2-6 parts of an astragalus membranaceus extract, 2-8 parts of propolis powder, 2-4 parts of a holly extract and 1-2 parts of a ginseng root extract. The skin care product capable of enhancing skin resistance and metabolic power contains a variety of safe and healthy raw materials in the absence of irritating chemicals, the irritation to the skin can be reduced, and moisture loss of the skin is effectively reduced; meanwhile, skin metabolism is effectively promoted, aging cells are repaired, cell absorption is promoted, skin resistance is enhanced, skin nutrition and moisture are fully regulated, and the needs of different people can be met.

The invention provides an anti-aging drug D / S for targeting senescent cells in a tissue micro-environment and application of the anti-aging drug D / S. Combined application of dasatinib and SRT2104 is adopted, and extremely excellent effects of reduction or removal of the senescent cells in the body are achieved, so that the drug is applied to removal damaged cells which are obtained after chemotherapy or radiation treatment and other damaged cells in the tissue micro-environment, and is also applied to elimination of cells which age naturally with ages, and the survival time of the body is prolonged.

The invention discloses a skin care cosmetic, particularly relates to an anti-allergy regenerative mask fluid. The fluid comprises, by weight percentage, 30%-60% of beta-glucan, 0.1%-0.5% of notoginsenoside, 0.01%-0.1% of astaxanthin, 0.1%-0.5% of Dipotassium Glycyrrhizate, 0.01%-0.2% of thickener, 0.1%-0.5% of methyl-p-hydroxy benzoate, 0.01%-0.05% of GM-BP and the balance being water. Natural plant extracts are adopted as the main ingredients for the fluid, and are specially selected to solve the problems of post-sunburn skin regeneration, allergy and moisture-keeping, wherein the beta-glucan not only has the function of keeping the skin moisture, but also can improve the skin elasticity and the ability to resist external stimulation, the notoginsenoside can nourish blood and alleviate pain, promote the regeneration of texture and inhibit inflamation. The astaxanthin is a natural anti-oxidant, and can reinforce the regenerative ability of cells and clean accumulated aging cells. The Dipotassium Glycyrrhizate has the anti-allergy and anti-stimulation effect with good percutaneous absorption effect.

The invention discloses a skin care essence based on human mesenchymal stem cells secreting active factors and a preparation method thereof. Human mesenchymal stem cells secrete a variety of large amounts of cytokines during the culture process, and these cytokines can penetrate deep into the basal layer of the skin to promote skin care. Tissue differentiation, angiogenesis and growth of granulation tissue promote the structural reconstruction and regeneration of damaged skin tissue, promote the removal of aging cells and the regeneration of cells, so as to maintain and restore the youthful state of the skin. Compared with the traditional skin care essence, the essence based on the active factors secreted by mesenchymal stem cells can fundamentally solve the aging problem of the skin, and has no toxic side effects. In the present invention, the human mesenchymal stem cell secretion is extracted after repeated starvation culture, and contains compound electrolyte injection and other ingredients. It can be used to relieve and repair skin aging problems caused by various reasons.

The present invention relates to modulators of FAS and DRs and their method of use in the treatment and prevention of diseases and pathologies related to accumulation of senescent cells during aging, such as cancer, chronic obstructive pulmonary disease (COPD), osteoarthritis, atherosclerosis, neurodegenerative diseases, diabetes, and many others. The present invention also relates to pharmaceutical compositions containing these compounds as well as various uses thereof.

The present disclosure provides compositions and methods for selectively killing senescent cells, wherein the composition comprises piperlongumine (PL) or derivative thereof. The selective killing of senescent cells may delay aging and / or treat age-related disorders.

The invention discloses a rapid quantitative senescence cell detection method based on senescence-associated beta-galactosidase. Culture solution is discarded from in-vitro cultured adherent cells, the adherent cells are cleaned by PBS (phosphate buffer solution), staining solution is added, the adherent cells are placed into a 37 DEG C carbon-dioxide-free incubator, all the staining solution is transferred into a centrifugal tube after staining and then discarded after centrifugation, a blue product is cleaned by the PBS, DMSO (dimethyl sulfoxide) is added to dissolve the blue product, the blue product is centrifuged, supernatant is taken, the absorbance of the solution at a 640nm position is measured, a standard curve between the number of senescence cells and the absorbance is built bythe same method, and the number of the senescence cells of a test sample can be known according to the standard curve after the absorbance is measured. The method has the advantages of high reproducibility and reliability, strong objectivity, fewer influence factors, high speed and the like, and animal and human senescence cells can be rapidly quantified.

The present disclosure provides compositions and methods for selectively killing senescent cells, wherein the composition comprises a compound of Formula (I) or a compound of Formula (II). The selective killing of senescent cells may delay aging and / or treat age-related disorders.

Provided is the discovery that dormant senescence prone cells (DSPCs) record an organism's exposure to genotoxic stress over the lifetime of the organism. The disclosure includes identifying DSPCs, using the amount of DSPCs to determine genotoxic dosage / dosimetry, and using these determinations in treatment and therapeutic approaches.

The invention discloses a preparation method for anti-aging cell preparation. The preparation method includes the following steps: adding the extracted bone marrow into a culture fluid, centrifuging and discarding the supernatant and inoculating the culture medium in a culture dish; replacing the liquid for the first time after 24-48 hours of primary culture, then replacing the liquid every 3d, sub-culturing at the ratio of 1:2 in 12-18h after the third times of replacement, adding the third generation of cultured mesenchymal stem cells to the normal saline for resuspending, and preparing the bone marrow mesenchymal stem cell preparation. By separating and culturing the bone marrow mesenchymal stem cells, and adding the bone marrow mesenchymal stem cells cultured in vitro to the normal saline, the bone marrow mesenchymal stem cell preparation can be obtained; The result shows that the cell preparation can improve the damage of cell morphology of the aged rat, reverse aging damage to some extent, and provide clues for studying the anti-aging mechanism of the bone marrow mesenchymal stem cells, as well as human anti-aging.

The invention provides an anti-aging medicine D / A targeting at aging cells in a tissue microenvironment and application of the anti-aging medicine D / A. Dasatinib is combined with aspirin, and a very excellent effect in reducing or eliminating aging cells of a body can be achieved, so that the medicine can be used for eliminating damaged cells such as damaged cells in the tissue microenvironment after chemotherapy or radiation therapy, and can be also used for eliminating cells aged naturally along with ages, and thus the living time of the body can be prolonged.

The invention discloses a body cream based on active factor extracts secreted by human mesenchymal stem cells and a preparation method thereof. Human mesenchymal stem cells secrete various and large amounts of cytokines during the culture process, and these cytokines can penetrate deep into the basal layer of the skin Promote skin tissue differentiation, angiogenesis and growth of granulation tissue, promote structural reconstruction and regeneration of damaged skin tissue, promote removal of aging cells and regeneration of cells, thereby maintaining and restoring youthful skin and reducing pigmentation. Compared with traditional body creams, body creams based on active factors secreted by mesenchymal stem cells can fundamentally solve the aging problem of the whole body skin without toxic side effects. In the present invention, the human mesenchymal stem cell secretion is extracted after repeated starvation culture, and contains compound electrolyte injection and other ingredients. It can be used for alleviating and repairing the skin aging problems of the whole body caused by various reasons.

The invention provides application of histone deubiquitinase in preparation of medicines for resisting aging and treating aging-related diseases, and relates to the technical field of biological medicines. The nucleotide sequence of the histone H2A deubiquitinase MYSM1 is shown as SEQ ID NO.1. Through verification, MYSM1 protein is deleted to cause remarkable aging of mice and induce aging-relateddiseases; and MYSM1 promotes DNA damage repair, blocks cell senescence and inhibits chronic inflammation. Adeno-associated viruses expressing murine MYSM1 are used as gene therapy results to prove that MYSM1 can significantly prolong the life of mice, reduce the process and quantity of aging cells, improve the normal functions of tissues and organs and reduce the occurrence and development of aging-related diseases; and it is proved that MYSM1 has good application prospects in delaying aging and treating aging-related diseases.

The invention provides an application of a cocoa extract in preparation of a medicine for resisting aging and inhibiting tumors, and discloses that the cocoa extract (CCE) acts on a tumor microenvironment through targeting to remove aging cells and can promote tumor inhibition by removing aging stromal cells. The CCE can also remove senescent cells in a senescence-related secretory phenotype (SASP) in a targeted manner, so that the SASP is inhibited. The CCE can also significantly prolong the life of animals, significantly prolong the lifetime of old people, and improve the life quality of animals.

The invention discloses application of high expression of lncRNA to inhibition of aging of umbilical cord mesenchymal stem cells. Clinical development and application of the MSCs, including UC-MSCs, cultured in vitro are restricted by the characteristic that the MSCs are prone to aging. D-galactose induction is a classical method for constructing an in-vitro senescence cell model. Research results of the invention show that UC-MSCs with high expression of lncRNA-1708 have relatively strong anti-aging activity, and also have strong in vitro proliferation activity at the same time. Therefore, the human umbilical cord mesenchymal stem cells can be cultured in vitro by a method for improving the expression level of the lncRNA-1708 so as to inhibit the aging of the human umbilical cord mesenchymal stem cells.

The present invention provides a method of detecting senescent cells or diagnosing cellular senescence in a subject wherein the level of one or more selected mi RNAs is quantified in a sample from said subject.

The invention belongs to the technical field of stem cell preparation, and particularly relates to a method and device for repairing stem cell aging. According to the method for repairing stem cell aging, mesenchymal stem cells are irradiated with red light, green light and yellow light, the wavelength of the red light is 609-657 nm, the wavelength of the green light is 485-567 nm, and the wavelength of the yellow light is 578-623 nm. The invention further provides a device for implementing the method. By adopting the method and the device provided by the invention, the senescence gene expression of the stem cells can be reduced, the number of senescence cells can be reduced, the production capacity of the mesenchymal stem cell exosome can be further remarkably improved, and the clinical application prospect is good.

The invention relates to a traditional Chinese medicine screening method, in particular relates to a method for screening anti-aging traditional Chinese medicine through telomere length. The method comprises the following steps of: accelerating human embryonic lung diploid fibroblasts to age under the action of tert-butly hydroperoxide to prepare an aging cell model; after molding, processing cells through various screened traditional Chinese medicine concentration compounds; after the processing is finished, extracting cell genome DNA, detecting the telomere length, and judging whether the drug is hot, namely the drug has an anti-aging activity according to the results.

Disclosed herein are methods and compositions for the identification of teloRNA marks to assess the differentiation status of an individual stem cell or a population of stem cells, to diagnose whether and to what extent a stem cell or stem cell culture has already initiated cell differentiation, and to monitor the differentiation status of an individual stem cell or a stem cell culture during passage. The use of these methods and compositions to monitor the pluripotency and differentiation status of a stem cell or stem cell culture during differentiation enables the identification of undifferentiated and pluripotent stem cells prior to the initiation of differentiation. The methods and compositions can also be used to assess and maintain cell viability; to identify cells or a population of cells that are in a state of poor cell health; and to reduce cell growth or treat a diseased cell including, for example, pre-cancerous cells, cancerous cells, apoptotic cells, aging cells, cells undergoing stress, and otherwise diseased or dysfunctional cells.

Body building anti-aging coix seed juice is provided and is prepared from following raw materials by weight: 30-35 parts of coix seed, 20-30 parts of millet, 10-15 parts of lily juice, 15-20 parts of Chinese wolfberry juice, 60-70 parts of mineral water, 50-60 parts of cranberry, 460-480 parts of Pu'er tea juice, 30-40 parts of black bean, 5-8 parts of glucose, 1-2 parts of lactic acid bacteria, and the like. The coix seed juice is disclosed. The coix seed, the millet, the black bean, and the like are added, so that the coix seed juice has a good function of nourishing bodies. The coix seed juice is smooth in taste. Extraction liquids of hairy antler, Chinese caterpillar fungus, a traditional Chinese medicine named as "Geshen" by the inventor, pseudo-ginseng, and the like have functions of tonifying qi and blood, strengthening the bones and muscles, tonifying spleen and stomach, resisting virus, removing ageing cells, and the like. The coix seed juice has body building and anti-aging functions.

The invention provides a stem cell active factor dressing for skin repair and a preparation method. Mask paper is soaked in mask liquid, and the mask liquid is fully absorbed by the mask paper to prepare the stem cell active factor dressing. The mask liquid contains EGF factors, trehalose, pullulan and a cogongrass extract. By adopting the trehalose, the pullulan and the cogongrass extract, skin stem cells can be moisturized immediately, the skin barrier is enhanced, and the skin elasticity is enhanced. By adopting the EGF factors, the proliferation ability of skin fibroblasts is improved, the ability of the skin fibroblasts to synthesize collagen is promoted, and the stem cell active factor dressing has good effects in the aspects of repairing cells, improving cell metabolism and activating aging cells.

A marine organism brightening and anti-aging toner relates to the field of skin care products, in particular to a marine organism brightening and anti-aging toner containing 20%-25% of a deep sea macroalgae extract, 15%-20% of ice sea cyanobacteria extract, astaxanthin 2.2%-3%, squalane 1.3%-2.3%, ginseng root extract 10%-15%, and the extract of the root of Paeonia lactiflora 12%-17%, hydrolyzed soybean protein 5.2%-6%, nicotinamide 0.5%-1.2%, citric acid 3.8%-4.5% and 30 per cent of the deep ocean water-35%. The formula has the advantages of mild and non-irritating, high safety, easy absorption by skin, good whitening and brightening, compact anti-aging effect, effective reduction of accumulation of aging cells, acceleration of metabolism of aged waste dark keratin, delay of skin aging, enhancement of skin elasticity, and restoration of crystal white and delicate aesthetic feeling to skin.

The invention discloses an aging cell model and a preparation method thereof. The preparation method of the aging cell model comprises the following steps of reducing the content or activity of multipotential stem cell ATF6, so as to obtain ATF6 deficiency multipotential stem cell; inducing the ATF6 dificiency multipotential stem cell, so as to obtain ATF6 deficiency mesenchymal stem cell, whereinthe ATF6 deficiency mesenchymal stem cell is the aging cell model; the multipotential stem cell is embryo stem cell or induced multipotential stem cell. The aging cell model has the advantages that the cell propagation ability is decreased, the recession speed is accelerated, the survival ability is decreased, the integration ability is decreased, and the protein homeostasis is unbalanced in thecell; the increasing of protein aggregate in the cell can be used for establishing an individualized medicine screening platform which is used for screening candidate (natural) compounds for regulating and maintaining adult stem cell protein homeostasis, and retarding cell ageing.

Disclosed in the present specification are a method for preparing senescent melanocytes, senescent cells prepared by the preparation method and a method for screening a senescence-alleviating or skin whitening material by using the senescent cells. The cells can be used for studying a cell senescence phenomenon and a pigmentation mechanism, which are caused by the accumulation of UV ray stimulation. Particularly, the cells are useful since a material having both senescence-alleviating and skin whitening effects can be screened.

The invention relates to a traditional Chinese medicine screening method and particularly relates to a method for screening an anti-aging traditional Chinese medicine by virtue of telomere length. The method comprises the following steps of: accelerating the aging of a diploid fibroblast cell of a human embryo lung under the action of teri-butyl hydroperoxide so as to manufacture an aging cell model; after the model is manufactured, treating the cell by use of various selected traditional Chinese medicine condensed compounds; and after the treatment is finished, extracting the genome DNA (Deoxyribonucleic Acid) of the cell, detecting the telomere length, and judging whether the traditional Chinese medicine is a febrile medicament, namely having anti-aging activity according to the result.