78 results about "Aging cell" patented technology

The invention discloses a label-free cell detection device and method based on light sheet illumination. The method includes the steps that a light sheet generation unit is used for shaping a laser beam into a uniform illumination light sheet with the micron-order thickness through a cylinder lens; the light sheet excites particles or a cell suspension placed in a sample micro-cavity chamber controlled by a precise displacement table to move; a form microscopic image and a two-dimensional light scattering pattern of a single particle or cell are recorded by a detector under the focusing mode and the defocusing mode through an objective lens; and a result is input into an imaging analysis system for image processing and recognizing and classifying. By means of the light sheet illumination method, a stimulation area can be effectively limited, background interference in light scattering imaging is inhibited, and effective stimulation of the single particle or cell and particle size discrimination of the sub-micron resolution ratio level are realized; and a light sheet illumination stimulation two-dimensional light scattering technology can avoid complex dyeing operation and a fluorescence signal detection process, and label-free detection and classification are carried out on aging cells. The label-free cell detection device and method based on light sheet illumination are high in applicability and capable of being popularized.

It is an object of the present invention to provide a solution for creating an effective method for retarding unhealthy manifestations brought by ageing of human beings (in particular, but not limited to the reduction of sexual activity and fertility, climax, changes in glucose tolerance, reduction of cognitive and mnestic functions, reduction of stress resistance, development of organ and tissue sclerosis) without directly affecting the genetic apparatus of the ageing cells.

According to the invention this task is solved by administration into the blood circulation of the agent which inactivates extracellular blood plasma DNA; the extracellular blood DNA inactivating agent can be embodied in the form of an extracellular blood plasma DNA destroying agent; said extracellular blood plasma DNA destroying agent can be embodied in the form of an DNase enzyme; the extracellular blood plasma DNA inactivating agent can also be embodied in the form of an extracellular blood plasma DNA binding agent; the extracellular blood plasma DNA binding agent can be embodied in the form of anti-DNA antibodies; the extracellular blood plasma DNA inactivating agent can be administered in the form of an enzyme modifying the chemical composition of extracellular blood plasma DNA; the extracellular blood plasma DNA inactivating agent can be embodied in the form of an agent that stimulates synthesis or activity of endogenous deoxyribonuclease, or an agent that stimulates the synthesis of antibodies which capable to bind extracellular blood plasma DNA.

The invention discloses a human-derived stem cell bioactive substance, its preparation and application. The method comprises: first extracting healthy human-derived stem cells, which are then subjected to in vitro culture and amplification, collecting stem cells of specific phases, and utilizing modern ultralow temperature as well as other biological technologies to prepare a stem cell bioactive substance group composition. Mainly coming from mesenchymal stem cells and hematopoietic stem cells, the stem cell substance group can be developed into products with a cytobiological activity function. When applied on human bodies, the products can activate body stem cells, repair or substitute damaged, pathological and aging cells, adjust the metabolic function of body cells, enhance body immunity, and delay aging, etc. The human-derived stem cell bioactive substance group composition in the invention boasts substantial application prospects in medicine, healthcare and beauty maintaining fields.

The invention provides an anti-aging drug D/S for targeting senescent cells in a tissue micro-environment and application of the anti-aging drug D/S. Combined application of dasatinib and SRT2104 is adopted, and extremely excellent effects of reduction or removal of the senescent cells in the body are achieved, so that the drug is applied to removal damaged cells which are obtained after chemotherapy or radiation treatment and other damaged cells in the tissue micro-environment, and is also applied to elimination of cells which age naturally with ages, and the survival time of the body is prolonged.

The invention discloses a skin care cosmetic, particularly relates to an anti-allergy regenerative mask fluid. The fluid comprises, by weight percentage, 30%-60% of beta-glucan, 0.1%-0.5% of notoginsenoside, 0.01%-0.1% of astaxanthin, 0.1%-0.5% of Dipotassium Glycyrrhizate, 0.01%-0.2% of thickener, 0.1%-0.5% of methyl-p-hydroxy benzoate, 0.01%-0.05% of GM-BP and the balance being water. Natural plant extracts are adopted as the main ingredients for the fluid, and are specially selected to solve the problems of post-sunburn skin regeneration, allergy and moisture-keeping, wherein the beta-glucan not only has the function of keeping the skin moisture, but also can improve the skin elasticity and the ability to resist external stimulation, the notoginsenoside can nourish blood and alleviate pain, promote the regeneration of texture and inhibit inflamation. The astaxanthin is a natural anti-oxidant, and can reinforce the regenerative ability of cells and clean accumulated aging cells. The Dipotassium Glycyrrhizate has the anti-allergy and anti-stimulation effect with good percutaneous absorption effect.

The invention discloses a skin care essence based on human mesenchymal stem cells secreting active factors and a preparation method thereof. Human mesenchymal stem cells secrete a variety of large amounts of cytokines during the culture process, and these cytokines can penetrate deep into the basal layer of the skin to promote skin care. Tissue differentiation, angiogenesis and growth of granulation tissue promote the structural reconstruction and regeneration of damaged skin tissue, promote the removal of aging cells and the regeneration of cells, so as to maintain and restore the youthful state of the skin. Compared with the traditional skin care essence, the essence based on the active factors secreted by mesenchymal stem cells can fundamentally solve the aging problem of the skin, and has no toxic side effects. In the present invention, the human mesenchymal stem cell secretion is extracted after repeated starvation culture, and contains compound electrolyte injection and other ingredients. It can be used to relieve and repair skin aging problems caused by various reasons.

The present disclosure provides compositions and methods for selectively killing senescent cells, wherein the composition comprises a compound of Formula (I) or a compound of Formula (II). The selective killing of senescent cells may delay aging and/or treat age-related disorders.

Provided is the discovery that dormant senescence prone cells (DSPCs) record an organism's exposure to genotoxic stress over the lifetime of the organism. The disclosure includes identifying DSPCs, using the amount of DSPCs to determine genotoxic dosage/dosimetry, and using these determinations in treatment and therapeutic approaches.

The invention discloses a preparation method for anti-aging cell preparation. The preparation method includes the following steps: adding the extracted bone marrow into a culture fluid, centrifuging and discarding the supernatant and inoculating the culture medium in a culture dish; replacing the liquid for the first time after 24-48 hours of primary culture, then replacing the liquid every 3d, sub-culturing at the ratio of 1:2 in 12-18h after the third times of replacement, adding the third generation of cultured mesenchymal stem cells to the normal saline for resuspending, and preparing the bone marrow mesenchymal stem cell preparation. By separating and culturing the bone marrow mesenchymal stem cells, and adding the bone marrow mesenchymal stem cells cultured in vitro to the normal saline, the bone marrow mesenchymal stem cell preparation can be obtained; The result shows that the cell preparation can improve the damage of cell morphology of the aged rat, reverse aging damage to some extent, and provide clues for studying the anti-aging mechanism of the bone marrow mesenchymal stem cells, as well as human anti-aging.

The invention provides an anti-aging medicine D/A targeting at aging cells in a tissue microenvironment and application of the anti-aging medicine D/A. Dasatinib is combined with aspirin, and a very excellent effect in reducing or eliminating aging cells of a body can be achieved, so that the medicine can be used for eliminating damaged cells such as damaged cells in the tissue microenvironment after chemotherapy or radiation therapy, and can be also used for eliminating cells aged naturally along with ages, and thus the living time of the body can be prolonged.

The invention discloses a body cream based on active factor extracts secreted by human mesenchymal stem cells and a preparation method thereof. Human mesenchymal stem cells secrete various and large amounts of cytokines during the culture process, and these cytokines can penetrate deep into the basal layer of the skin Promote skin tissue differentiation, angiogenesis and growth of granulation tissue, promote structural reconstruction and regeneration of damaged skin tissue, promote removal of aging cells and regeneration of cells, thereby maintaining and restoring youthful skin and reducing pigmentation. Compared with traditional body creams, body creams based on active factors secreted by mesenchymal stem cells can fundamentally solve the aging problem of the whole body skin without toxic side effects. In the present invention, the human mesenchymal stem cell secretion is extracted after repeated starvation culture, and contains compound electrolyte injection and other ingredients. It can be used for alleviating and repairing the skin aging problems of the whole body caused by various reasons.

The invention provides an application of a cocoa extract in preparation of a medicine for resisting aging and inhibiting tumors, and discloses that the cocoa extract (CCE) acts on a tumor microenvironment through targeting to remove aging cells and can promote tumor inhibition by removing aging stromal cells. The CCE can also remove senescent cells in a senescence-related secretory phenotype (SASP) in a targeted manner, so that the SASP is inhibited. The CCE can also significantly prolong the life of animals, significantly prolong the lifetime of old people, and improve the life quality of animals.

The invention belongs to the technical field of stem cell preparation, and particularly relates to a method and device for repairing stem cell aging. According to the method for repairing stem cell aging, mesenchymal stem cells are irradiated with red light, green light and yellow light, the wavelength of the red light is 609-657 nm, the wavelength of the green light is 485-567 nm, and the wavelength of the yellow light is 578-623 nm. The invention further provides a device for implementing the method. By adopting the method and the device provided by the invention, the senescence gene expression of the stem cells can be reduced, the number of senescence cells can be reduced, the production capacity of the mesenchymal stem cell exosome can be further remarkably improved, and the clinical application prospect is good.

Disclosed herein are methods and compositions for the identification of teloRNA marks to assess the differentiation status of an individual stem cell or a population of stem cells, to diagnose whether and to what extent a stem cell or stem cell culture has already initiated cell differentiation, and to monitor the differentiation status of an individual stem cell or a stem cell culture during passage. The use of these methods and compositions to monitor the pluripotency and differentiation status of a stem cell or stem cell culture during differentiation enables the identification of undifferentiated and pluripotent stem cells prior to the initiation of differentiation. The methods and compositions can also be used to assess and maintain cell viability; to identify cells or a population of cells that are in a state of poor cell health; and to reduce cell growth or treat a diseased cell including, for example, pre-cancerous cells, cancerous cells, apoptotic cells, aging cells, cells undergoing stress, and otherwise diseased or dysfunctional cells.

Body building anti-aging coix seed juice is provided and is prepared from following raw materials by weight: 30-35 parts of coix seed, 20-30 parts of millet, 10-15 parts of lily juice, 15-20 parts of Chinese wolfberry juice, 60-70 parts of mineral water, 50-60 parts of cranberry, 460-480 parts of Pu'er tea juice, 30-40 parts of black bean, 5-8 parts of glucose, 1-2 parts of lactic acid bacteria, and the like. The coix seed juice is disclosed. The coix seed, the millet, the black bean, and the like are added, so that the coix seed juice has a good function of nourishing bodies. The coix seed juice is smooth in taste. Extraction liquids of hairy antler, Chinese caterpillar fungus, a traditional Chinese medicine named as "Geshen" by the inventor, pseudo-ginseng, and the like have functions of tonifying qi and blood, strengthening the bones and muscles, tonifying spleen and stomach, resisting virus, removing ageing cells, and the like. The coix seed juice has body building and anti-aging functions.

The invention provides a stem cell active factor dressing for skin repair and a preparation method. Mask paper is soaked in mask liquid, and the mask liquid is fully absorbed by the mask paper to prepare the stem cell active factor dressing. The mask liquid contains EGF factors, trehalose, pullulan and a cogongrass extract. By adopting the trehalose, the pullulan and the cogongrass extract, skin stem cells can be moisturized immediately, the skin barrier is enhanced, and the skin elasticity is enhanced. By adopting the EGF factors, the proliferation ability of skin fibroblasts is improved, the ability of the skin fibroblasts to synthesize collagen is promoted, and the stem cell active factor dressing has good effects in the aspects of repairing cells, improving cell metabolism and activating aging cells.

The invention relates to a traditional Chinese medicine screening method and particularly relates to a method for screening an anti-aging traditional Chinese medicine by virtue of telomere length. The method comprises the following steps of: accelerating the aging of a diploid fibroblast cell of a human embryo lung under the action of teri-butyl hydroperoxide so as to manufacture an aging cell model; after the model is manufactured, treating the cell by use of various selected traditional Chinese medicine condensed compounds; and after the treatment is finished, extracting the genome DNA (Deoxyribonucleic Acid) of the cell, detecting the telomere length, and judging whether the traditional Chinese medicine is a febrile medicament, namely having anti-aging activity according to the result.

The invention discloses a facial mask with the effect of enhancing skin elasticity, which can effectively remove free radicals, increase the content of antioxidant enzymes, and further reduce free radicals Based on the same peroxide content, it can also protect the cell structure and prevent cell damage. The synergistic effect between the ingredients makes the facial mask of the present invention have a significant antioxidant effect; The expression of the gene can regenerate cell elastin and restore the elasticity of some aging cells. Combined with the supplement of nutrients in the extract of the predictor, it can accelerate the process of cell repair. Finally, wild grape gallic acid can effectively remove harmful metabolites and reduce the accumulation of harmful substances. The resulting changes in skin texture prevent further cell aging. In summary, the effect of enhancing skin elasticity is achieved.

Disclosed are methods and compositions for reducing the epigenetic age of organisms, especially that of adult humans, which provide for proliferating endogenous stem cells, removing aberrant epigenetic marks from chromosomes and mitochondrial DNA, and replacement of senescent cells.

The invention provides use of a CDK inhibitor in preparation of drugs for inducing aging of precancerous cells of an object, use of the CDK inhibitor in preparation of drugs for preventing or treating a precancerous lesion or cancer of the object, and use of the CDK inhibitor in preparation of drugs for inducing immune response of the object to aged cells.