Application of high expression of lncRNA in inhibiting aging of umbilical cord mesenchymal stem cells

A stem cell and aging technology, applied in the field of stem cells, can solve the problems of MSCs easy aging, restricting clinical development and application, etc., and achieve the effect of strong anti-aging activity and strong in vitro proliferation activity

Inactive Publication Date: 2022-03-22
淮安泰凯睿医药科技有限公司
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Problems solved by technology

[0004] However, the senescence characteristics of MSCs including UC-MSCs cultured in vitro restrict their clinical development and application

Method used

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  • Application of high expression of lncRNA in inhibiting aging of umbilical cord mesenchymal stem cells
  • Application of high expression of lncRNA in inhibiting aging of umbilical cord mesenchymal stem cells
  • Application of high expression of lncRNA in inhibiting aging of umbilical cord mesenchymal stem cells

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Embodiment Construction

[0014] The substantive content of the present invention will be specifically described below in conjunction with the embodiments, but those skilled in the art should know that the protection scope of the present invention should not be limited to the specific embodiments.

[0015] 1. Experimental materials

[0016] Fetal bovine serum and DMEM / F12 medium were purchased from Gibco, and double antibodies, PBS and trypsin were purchased from Beyontib. lncRNA-1708 mimic and lncRNA-1708 NC mimic were synthesized and provided by Shanghai Gemma Biotechnology Co., Ltd. FITC or PE-labeled mouse anti-human CD90, CD105, CD73, CD14, CD34 and CD45 antibodies and related isotype controls were purchased from eBioscince, USA. Lipofectamine 2000 was purchased from Invitrogen. TRIzol kit was purchased from Thermo Fisher. Reagent materials related to Western Blot were purchased from Biyuntian Biotechnology, etc.

[0017] 2. Experimental method

[0018] 1. Isolation and culture of UC-MSCs

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Abstract

The invention discloses application of high expression of lncRNA to inhibition of aging of umbilical cord mesenchymal stem cells. Clinical development and application of the MSCs, including UC-MSCs, cultured in vitro are restricted by the characteristic that the MSCs are prone to aging. D-galactose induction is a classical method for constructing an in-vitro senescence cell model. Research results of the invention show that UC-MSCs with high expression of lncRNA-1708 have relatively strong anti-aging activity, and also have strong in vitro proliferation activity at the same time. Therefore, the human umbilical cord mesenchymal stem cells can be cultured in vitro by a method for improving the expression level of the lncRNA-1708 so as to inhibit the aging of the human umbilical cord mesenchymal stem cells.

Description

technical field [0001] The invention belongs to the field of stem cells, and in particular relates to the use of high expression of lncRNA to inhibit senescence of umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (mesenchymal stem cells, MSCs) are a kind of cells with high self-renewal, multi-lineage differentiation and unique immunoregulatory properties, which can regulate the immune system by interacting with cells of the innate and adaptive immune systems and secreting cells. Sex factors play an immunomodulatory role. Mesenchymal stem cell exosomes (exosomes from mesenchymal stem cells, MSCs-Exo) is a secreted substance that MSCs function. Studies have shown that MSCs-Exo possess multiple activities, such as tissue repair, immune regulation, and disease treatment, which have been paid more and more attention. [0003] Umbilical cord-mesenchymal stem cells (UC-MSCs) have the characteristics of sufficient sources, convenient mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N5/0775C12N15/113
CPCC12N5/0668C12N15/113C12N2310/10
Inventor 不公告发明人
Owner 淮安泰凯睿医药科技有限公司
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