Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fumosochromone A with anti-aging activity and preparation method thereof

A rose-colored, anti-aging technology, which is applied in the field of extraction and preparation of biological products, can solve the problems that have not yet been reported on the preparation, identification and biological activity research of rose-nicotin compounds

Active Publication Date: 2021-07-06
ANHUI AGRICULTURAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the long-term co-evolution with insects, this type of fungus can produce a large number of new bioactive compounds. However, there are still few studies on the metabolites of this type of fungus. to report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fumosochromone A with anti-aging activity and preparation method thereof
  • Fumosochromone A with anti-aging activity and preparation method thereof
  • Fumosochromone A with anti-aging activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation steps of fumocyanin A with anti-aging activity are as follows:

[0050] (1) Preparation of solid culture

[0051](1.1) Primary strain culture

[0052] The preserved Cordyceps fumigatus ( Cordyceps fumosorosea ) water liquid strains were inoculated on potato agar (PDA) slant medium, each slant was inoculated with 10 μL, cultured at 20°C for 5 days, and the spores were collected to obtain the first-grade strains;

[0053] PDA medium is potato dextrose medium, its formula is: 6g potato powder, 20g glucose, 15g agar, add water to 1000mL.

[0054] (1.2) Secondary strain culture

[0055] Make the first-grade bacteria with water to a concentration of 10 5 ~10 7 spores / mL of spore suspension, inoculate 200 μL per plate onto a pre-prepared PDA plate (the size of the PDA plate is 9×100mm), culture at 20°C for 6 days at constant temperature, collect spores, and obtain secondary strains . The composition of PDA medium is the same as step (1).

[0056] (1.3) ...

Embodiment 2

[0068] The preparation steps of fumocyanin A with anti-aging activity are as follows:

[0069] (1) Preparation of solid culture

[0070] (1.1) Primary strain culture

[0071] The preserved Cordyceps fumigatus ( Cordyceps fumosorosea ) water liquid strains were inoculated on potato agar (PDA) slant medium, each slant was inoculated with 30 μL, cultured at 30°C for 10 days, and the spores were collected to obtain the first-grade strains;

[0072] PDA medium is potato dextrose medium, its formula is: 6g potato powder, 20g glucose, 15g agar, add water to 1000mL.

[0073] (1.2) Secondary strain culture

[0074] Make the first-grade bacteria with water to a concentration of 10 7 spores / mL of spore suspension, inoculate 400 μL per plate onto a pre-prepared PDA plate (the size of the PDA plate is 9×100mm), culture at 30°C and constant temperature for 12 days, collect spores, and obtain secondary strains . The composition of PDA medium is the same as step (1).

[0075] (1.3) Ter...

Embodiment 3

[0087] The operation steps of the fumocyanin A with anti-aging activity and the preparation method are as follows:

[0088] (1) Preparation of solid culture

[0089] (1.1) Primary strain culture

[0090] The preserved Cordyceps fumigatus ( Cordyceps fumosorosea ) water liquid strains were inoculated on potato agar (PDA) slant medium, each slant was inoculated with 20 μL, cultured at 25°C for 8 days, and the spores were collected to obtain the first-grade strains;

[0091] PDA medium is potato dextrose medium, its formula is: 6g potato powder, 20g glucose, 15g agar, add water to 1000mL.

[0092] (1.2) Secondary strain culture

[0093] Make the first-grade bacteria with water to a concentration of 10 6 spores / mL of spore suspension, inoculate 300 μL per plate onto a pre-prepared PDA plate (the size of the PDA plate is 9×100mm), culture at 25°C and constant temperature for 10 days, collect spores, and obtain secondary strains . The composition of PDA medium is the same as s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to fumosochromone A with anti-aging activity and a preparation method thereof, and belongs to the technical field of extraction and preparation of biological products. The fumosochromone A is a colorless oily substance, is a novel compound, and is not reported in literatures yet. The compound provided by the invention can be obtained by culturing Cordyceps fumosorosea and then extracting and separating the Cordyceps fumosorosea. An activity determination result of the fumosochromone A shows that the fumosochromone A has relatively strong anti-aging activity; when the concentration of the fumosochromone A is 200 mg / mL, the life of experimental nematodes can be prolonged by 11.3%; the egg laying rate of the nematodes is increased by 73.1%; after 7 days of thermal shock treatment at 35 DEG C, the survival rate of a 200 mg / mL fumosochromone A treatment group is more than two times that of a control group; after the nematodes are treated by 200 mg / mL fumosochromone A for 15 days, the nematodes with high activity in the treated group are more than three times that of the control group. The compound has the potential of preparing anti-aging health care products and drugs.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of biological products, and relates to the extraction, purification, structure identification and activity determination of fumosoroseain A with anti-aging activity. Background technique [0002] Fumosin A is obtained from a strain of Cordyceps fumigatus ( Cordyceps fumosorosea ) is a new compound-type alkaloid compound isolated and prepared from the culture, which has biological activities such as anti-aging. [0003] The anamorph of Cordyceps fumoscens exists in the soil and parasitizes on insects, formerly known as I. Isaria fumosorosea ) or Paecilomyces fumigatus ( Paecilomyces fumosorosea ). The fungus has a wide range of hosts and is susceptible to aphids, whiteflies, beetles, termites, wasps and various Lepidoptera pests. Therefore, Cordyceps fumigatus is an important biological control fungus. Due to the long-term co-evolution with insects, this type of fungus can ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07C229/22C07C227/40C12P13/00C12N1/14A61P39/06C12R1/645C12R1/79
CPCC07C229/22C07C227/40C12P13/001C12N1/14A61P39/06Y02A50/30
Inventor 胡丰林陆瑞利周雪尉杰
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com