Method and device for repairing aging of stem cells

A stem cell and quality stem cell technology, applied in the field of repairing stem cell aging, to reduce the expression of aging genes, have a good application prospect, and improve yield and quality.

Pending Publication Date: 2022-04-29
泸州君益生物医学研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the adverse effects of various drugs on organisms such as biological toxicity or side effects to a certain exten

Method used

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  • Method and device for repairing aging of stem cells
  • Method and device for repairing aging of stem cells
  • Method and device for repairing aging of stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The method for repairing stem cell senescence

[0031] Take the adipose-derived mesenchymal stem cells and place them in a transparent syringe. The adipose-derived mesenchymal stem cells are irradiated for 30 minutes under the LED red light source, the LED green light source and the LED yellow light source at a speed of 30 rpm. The wavelength of the red light source is 620-625nm. The light intensity is 120mW / sr; the wavelength of LED green light source is 510-520nm, and the light intensity is 25mW / sr; the wavelength of LED yellow light source is 585-590nm, and the light intensity is 175mW / sr.

Embodiment 2

[0032] Example 2 Device for Repairing Stem Cell Aging

[0033] This embodiment provides a device for repairing stem cell aging (cell therapy conversion instrument), its structure is as follows: figure 1 , 2 As shown, it includes a base, an outer cover 1 and a lamp tube 2 inside the outer cover. A container inlet 3 is arranged on one side of the outer cover 1. A liquid crystal display 4 and an instrument start key 10 are arranged on the surface of the outer cover 1. The inside of the lamp tube 2 is equipped with a The cavity 7 of the adipose-derived mesenchymal stem cell container (such as a transparent syringe), one end of the cavity 7 is open. Said opening is opposite to the container inlet 3 . A light source group for illuminating the interior of the cavity 7 is provided on the lamp tube 2, and the light source group is an array composed of red LED light sources, green LED light sources and yellow LED light sources. There is a control board inside the outer cover 1, the l...

experiment example 1

[0043] Experimental Example 1 Repair of Adipose-derived Mesenchymal Stem Cells

[0044] 1. Instrument

[0045] The cell therapy conversion instrument described in Example 2.

[0046] 2. Processing method

[0047] 1. Isolation of adipose-derived mesenchymal stem cells

[0048] (1) The mice were killed by dislocation, and the animals were sterilized with 70% ethanol for 5-10 minutes. Dissect the abdominal skin, expose the subcutaneous adipose tissue, and remove the subcutaneous adipose tissue using sterile forceps and scissors.

[0049] (2) Place the extracted tissue in a 6 cm pre-weighed Petri dish. Weigh the tissue (Ng) with an analytical balance.

[0050] (3) Add room temperature 1×N ml (wherein N is the weight of adipose tissue in grams) MSC isolation medium to the culture dish containing adipose tissue.

[0051] (4) Mince the adipose tissue with sterile scissors until fine lumps are formed, using a 1 ml filter with a severed end, accurately transfer the minced tissue ...

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Abstract

The invention belongs to the technical field of stem cell preparation, and particularly relates to a method and device for repairing stem cell aging. According to the method for repairing stem cell aging, mesenchymal stem cells are irradiated with red light, green light and yellow light, the wavelength of the red light is 609-657 nm, the wavelength of the green light is 485-567 nm, and the wavelength of the yellow light is 578-623 nm. The invention further provides a device for implementing the method. By adopting the method and the device provided by the invention, the senescence gene expression of the stem cells can be reduced, the number of senescence cells can be reduced, the production capacity of the mesenchymal stem cell exosome can be further remarkably improved, and the clinical application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of stem cell preparation, and in particular relates to a method and a device for repairing aging of stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are cells derived from adult tissues with self-renewal and multidirectional differentiation capabilities, and are widely found in bone marrow, skeletal muscle, umbilical cord, fat, skin and other tissues and organs. Mesenchymal stem cells have great application value in organ regeneration, repair and disease treatment. [0003] Senescence or biological aging is the gradual deterioration of the functional characteristics of an organism. Senescence refers to the aging of cells, and can also refer to the aging of the whole body. Organism senescence involves increased mortality and / or decreased fecundity as the organism ages, at least later in the organism's life cycle. Regenerative medicine has broadly focused on the development of cell th...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12M3/00
CPCC12N5/0667C12M31/12C12M35/02C12N2529/10
Inventor 吴剑波
Owner 泸州君益生物医学研究有限公司
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