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Method for screening anticancer traditional Chinese medicine through telomerase

A technology of telomerase and traditional Chinese medicine, applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., can solve the problem of cells entering the apoptosis process, and achieve the effect of increasing survival time

Inactive Publication Date: 2013-07-10
CHANGZHOU ADAM BIOTECH
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AI Technical Summary

Problems solved by technology

At the same time, it has been reported that most tumor cells maintain their shortest length due to the action of telomerase during the reproduction process, so that the cells cannot enter the apoptosis program due to the final loss of telomeres, so the reproduction of tumor cells is endless

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Reagents and Cells

[0015] a. Sample: 10 groups of cold traditional Chinese medicine

[0016] b. Positive control traditional Chinese medicine: telomerase inhibitor berberine-berberine, moderate strength telomerase inhibitor, IC50 value is about 35μmol / L);

[0017] c. Telomerase positive cells: HeLa cells (+), breast cancer MCF-7 cells (+), leukemia cells U937 (+), leukemia cells HL60 (+), lymphoma cells CA46 (+).

[0018] ) cell culture and digestion passaging

[0019] Tumor cells were cultured in 1640 medium containing 10% calf serum at 37°C, 5% CO 2 Cultivate in an incubator. When it is more than 90% full, discard the original medium in the bottle, wash it twice with PBS, discard it, add 2ml of 0.25% trypsin solution, digest in a 37°C incubator for 3min, and turn to the scale Centrifuge at 1000rpm for 3min in the centrifuge tube, then add 2ml of fresh complete medium to resuspend, then count with Cell Counter.

[0020]

[0021] 3) MTT method to determine the ef...

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Abstract

The invention relates to a traditional Chinese medicine screening method, in particular relates to a method for screening anti-aging traditional Chinese medicine through telomere length. The method comprises the following steps of: accelerating human embryonic lung diploid fibroblasts to age under the action of tert-butly hydroperoxide to prepare an aging cell model; after molding, processing cells through various screened traditional Chinese medicine concentration compounds; after the processing is finished, extracting cell genome DNA, detecting the telomere length, and judging whether the drug is hot, namely the drug has an anti-aging activity according to the results.

Description

technical field [0001] The invention relates to a method for screening medicines, in particular to a method for screening anticancer traditional Chinese medicines through telomerase. Background technique [0002] Telomere is a small piece of DNA-protein complex that exists at the end of linear chromosomes in eukaryotic cells. Together with telomere binding proteins, it forms a special "cap" structure that can maintain the integrity of chromosomes. Telomere DNA is composed of simple highly repetitive DNA sequences, and the DNA strands along the 5' to 3' direction at the ends of chromosomes are rich in two nucleotides, guanine (G) and thymine (T). In yeast and human, the telomere sequences are C1-3A / TG1-3 and TTAGGG / CCCTAA, respectively, and many proteins bind to telomere DNA. The main functions of telomere DNA are: first, to protect chromosomes from being degraded by nucleases; second, to prevent mutual fusion of chromosomes; third, to provide substrates for telomerase, to s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/02
Inventor 叶亚东路易斯伊格纳罗张娟全利郝小江朱兆云滕凌朱文华胡娟肖慧潘鹂张俊杨紫俊
Owner CHANGZHOU ADAM BIOTECH
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