54 results about "Cell ageing" patented technology

The method relates to a method for preparing collagen peptide with a fish skin, comprising the steps of: washing, draining and cutting the fish skin into pieces, soaking the fish skin pieces in 75% ethanol for 15 min, then taking them out and washing by soaking them in sterile water for 3-5 times and then draining; putting the fish skin pieces into a fermenter so as to keep the skin pieces with a concentration of 4%-8%, inoculating strains with an amount of 1%-5% and a cell age of 14h-18h for fermentation at a temperature of 30-37 DEG C for 48-56h, with 30%-40% of dissolved oxygen, and after fermentation filtering the fermentation liquor to remove thalluses, then conducting enzyme destruction, vacuum concentration and spray drying to the fermentation liquor, thus obtaining collagen peptide. Compared with present methods for preparing collagen peptide with a fish skin, the method of the invention prepares collagen peptide directly through the fermentation method. Thus the preparation process of collagen peptide is simplified, and the production cost is reduced, without environmental pollution. Therefore, the method of the invention has good application prospect.

This invention is directed to a skin care composition comprising a laminriacea (brown seaweed) extract and the use of said composition in the treatment of skin aging signs Specifically, it was discovered that a crude extract of the laminariacea Saccharina Longicruris has a strong potential for anti-senescence and anti-cellular aging effect and is efficient to restore the contraction of the collagen network of aged cell lattices to the level of contraction of young cells.

The invention discloses a method for preparing a population of?human pluripotent stem cells and the application thereof. The preparation of stem cells is characterized by comprising the following steps: CD151<+>, CD31<->, Sox<2+> pluripotent stem cells are separated and collected from human umbilical cord and or placenta tissues; the cells adhere to grow in a culture vessel under a predetermined condition and expand through passage 20 or above to be still stable in gene expression. The population of cells of this invention do not form teratoma after injection into animals. The human pluripotent stem cells highly express CD151, OCT4 and Sox-2 as specific markers of embryonic stem cells, as well as specific markers of epidermic cells, endothelial cells, thrombocytes, dendritic cells, while lack expression of CD31, CD34, CD45 and HLA-II. The pluripotent stem cells are also characterized as being able to adhere to tissue culture plastic and having the potential to differentiate into three germ layers: endoderm, mesoderm and ectoderm. These pluripotent stem cells are able to be used as carrier cells of gene therapy and for the treatment of diseases caused by cell damage or cell aging. The present invention provides a method of isolating, purifying and culturally expanding of a population of human pluripotent stem cell for preparing the high purity injection preparation. The preparation of stem cells has a good therapeutic effect on the treatment of diseases caused by cell damage or cell aging in animal and human clinical trials. The preparation also has no toxic side effect and no immune rejection.

The invention relates to a tissue engineering meniscus repair sheet and a preparation method thereof. The prepared tissue engineering meniscus repair sheet comprises an acellular meniscal thin sheet, seed cells and growth factor slow-release carriers, wherein blind micro vias are uniformly distributed on two surfaces of the acellular meniscal thin sheet, and the seed cells and the growth factor slow-release carriers are combined on two surfaces of the acellular meniscal thin sheet. The blind micro vias distributed on the acellular meniscal thin sheet increase the area of a support material, and can be used as a storage pool of the seed cells and the growth factor slow-release carriers, so that the seed cells and the growth factor slow-release carriers can be firmly attached and are not easy to fall off. Nutrition can be persistently released through the slow-release carrier by multiple growth factors, the cell amplification efficiency is improved, the cell phenotype is also maintained, cell ageing is inhibited, and fusion of the tissue engineering meniscus repair sheet and self tissue is promoted. The prepared tissue engineering meniscus repair sheet has the structure characteristic similar to the structure characteristic of natural meniscus, and is applicable to repair meniscus injure and induce regeneration of meniscus fibrous cartilage tissue.

The invention belongs to the field of cell detection and provides an estimation method of charging time. The estimation method comprises following steps of acquiring different cell aging degrees, different charging temperatures and charging curves under different starting electric quantities in an experiment manner; averaging pints on multiple groups of charging curves under the same experiment condition, and using a charging curve corresponding to the mean as a typical charging curve; storing the typical charging curve into a terminal device, and selecting an optimal typical charging curve based on the cell aging degrees, the current charging temperature and the starting electrical quantity of the terminal device; and based on the optimal typical charging curve, acquiring charging estimation time from the starting electrical quantity to the target electrical quantity. In this way, characteristics of charging curves of the cell are considered and effects on charging processes imposed by environment factors are also considered, so accuracy of the charging estimation time is ensured.

The invention discloses an integrated cell age analysis system. According to the integrated cell age analysis system, integrated evaluation is carried out according to the personal data and the telomere detection data, corresponding personal aging degree evaluation and the prevention and improvement advice information can be acquired rapidly and accurately according to the cell age and cell aging index analysis data, a body age and the potential risk of a user can be known on the basis of the information and the cell aging analysis data, getting to know about whether a physiological state is in an in-advance aging trend is facilitated, improvement in life styles and environment factors can be made through reliable and proper personalized aging prevention improvement health care countermeasures, an abnormal body aging rate can be avoided, and purposes of health improvement and relevant disease reduction are realized.

The invention relates to a culture preserving method, which comprises the following steps: (1) inoculating the culture to be preserved in a sterilized bran culture medium, wherein the formula of the culture medium comprises the following components in percentage by weight: 50-60% of bran, 35-45% of pulverized chaff, 1-2% of sodium nitrate, 1-2% of urea, 1-2% of rice flour and 0.2-1% of biotin nutrition additive, adding de-ionized water of which the weight is the same as that of the mixture, and mixing evenly; (2) when mycelia grows to a required cell age, directly and immediately adding a frozen dry solution into an ampoule tube and mixing with the culture evenly without separating the mycelia to prepare a bacterial suspension, wherein the formula of the frozen dry solution comprises the following components by weight: 10-20 grams of skimmed milk, 0.2-0.5 gram of agar powder, 0.2-0.5 gram of reduced glutathione, 0.1-0.5 gram of Arabic gum and 0.5-1 gram of tert-butyl alcohol, and determining the volume to be 100 milliliters with de-ionized water; (3) then cooling at the cooling rate of 2 DEG C / minute for freeze drying; (4) after freeze drying, vacuumizing the ampoule tube containing the bacteria and then sealing; and (5) preserving the obtained tube at a low temperature.

The invention discloses a Pycnogenol / rhEGF composition skin care cosmetic and a preparation method thereof. The Pycnogenol / rhEGF composition skin care cosmetic is prepared from six-phase materials, wherein, two main components comprise, by weight, 1.4 to 1.6% of Pycnogenol and 0.01 to 0.1% of rhEGF. According to the invention, a high efficiency antioxidation effect of Pycnogenol is utilized to remove free radicals generated by metabolism of a living organism or by physical radiation and chemical substances, to eliminate damage of free radicals to cell membranes and to protect the quantity andproliferation capability of EGFR acceptor protein on the cell membranes; exogenous rhEGF is supplemented so as to maintain the normal physiological level of aging cells EGF and enhance anti-interference ability of skin, thereby effectively repressing cell aging so as to prevent and delay aging of skin.

The invention relates to the field of cosmetics, in particular to a cosmetic composition rich in collagen tripeptide, a skin care cosmetic, application and a preparation method. The provided cosmeticcomposition rich in the collagen tripeptide is prepared from 5-10 parts of collagen tripeptide, 1-3 parts of a grape seed extract, 1-2 parts of hydrolyzed protein, 1-2 parts of a hydrolyzed rice extract and 0.5-1 part of a chlorella extract. The active substances cooperatively complement each other, the cosmetic composition formed through specific matching can provide nutrients and repairing for the human body skin in all directions, the effects of preserving moisture and resisting oxidation are achieved, and the function is obvious. Therefore, the prepared cosmetic composition can obviously delay cell aging and is an efficient and anti-aging skin care product.

The invention discloses health care drinking water, and particularly relates to nano platinum drinking water. The nano platinum drinking water is prepared from nano platinum with grain size of less than or equal to 3nm and common drinking water, and the nano platinum content is between 0.4 and 1.6ppm. The nano platinum drinking water contains platinum(Pt) which is called king of noble metal, so that the nano platinum drinking water has multiple efficacies of improving disease resistant capability, resisting oxidation, inhibiting bacteria, promoting blood circulation, enforcing metabolism, resisting cell ageing, preventing cancer and resisting cancer. Moreover, the invention also relates to a method for preparing the nano platinum drinking water.

The invention relates to skin soothing and regenerating protein film cream and a preparation method of the skin soothing regenerating protein film cream, relating to the technical field of cosmetics. The skin soothing regenerating protein film cream is prepared from the following components: water, neopentyl glycol diheptanoate, butanediol, glycerol, an oat extract, fucogel-1, glycosphingolipids, wheat fine powder lipid, a plankton extract, a mirabilis jalapa extract, a hydrolyzed egg shell membrane and the like. The skin soothing regenerating protein film cream can permeate to the stratum corneum of skin and form a reticular protective film in the skin, so that the problems of ultraviolet ray invasion and skin dryness and ageing can be solved, meanwhile, the synthesis of collagens from fibroblasts is promoted, therefore, the formation of skin wrinkles is reduced, the skin aging is resisted, the skin elasticity is improved, the skin is enabled to be tender, fine and smooth, as well as full and plump, and is full of beautiful youth gloss, the outside contamination resistance of the skin is enhanced, the self-protection ability of the skin is enhanced, the cell ageing is prevented, and thus the skin ageing is delayed, the youthful vigor of the skin is recovered, the skin is tender and full, and the beautiful skin with the real lasting health is realized from inside to outside.

The invention relates to an anti-wrinkle skin-tightening composition, an anti-wrinkle skin-tightening essence and a preparation method thereof. The anti-wrinkle skin-tightening composition comprises 0.05-3 parts of palmitoyl tetrapeptide-7, 0.5-8 parts of palmitoyl tripeptide-1, 0.1-3 parts of fibronectin and 0.1-6 parts of cannabidiol. Due to the mutual action of the components, the anti-wrinkleskin-tightening composition inhibits an inflammation reaction of skin and then reduces the cell aging apoptosis speed, meanwhile, sufficient nutrient substances are provided for the skin, nutrition metabolism of cells is improved, the skin is moisturized, cell regeneration is promoted, the regeneration and degradation balance of the cells is maintained, and the skin is healthy and active. The anti-wrinkle skin-tightening composition has a relatively strong free radical inhibiting effect, further maintains the environmental balance in the cells, improves the cell regeneration capability and improves the anti-wrinkle skin-tightening effects.

The invention discloses a pleurotus eryngii liquid strain with small and many mycelium pellets and a fermentation method of the pleurotus eryngii liquid strain, and belongs to the technical field of pleurotus eryngii cultivation. Chinese herb extracts capable of improving cold resistance, heat irritability resistance, immunity and bacterium-resisting capacity of the strain are scientifically compounded in culture mediums for liquid seeds, shaking flask seeds, seeding tank seeds and fermentation tank liquid strains in all stages, and meanwhile wood powder which has the feedstock adaptability and has the function of an antifoaming agent is scientifically compounded; manners of supplemental inoculation and segmentation variable temperature fermentation are adopted, and therefore the difference between the strain cell age and the multiplication algebra is shortened; and packaged chitosan with a special function is properly fed, and the pleurotus eryngii liquid strain with the thick and solid fermentative hyphae, high activity, small mycelium pellet diameter, large density and uniform distribution is cultured. The density of the mycelium pellets ranges from 4.5*10<5>piece / L to 5.5*10<5>piece / L, the diameter of the mycelium pellets ranges from 0.5mm to 0.7mm, the dry weight of the mycelium pellets ranges from 90g / L to 100g / L, the germination time ranges from 4 hours to 5 hours, and the exopolysaccharide ranges from 3.0g / L to 3.5g / L.

The methods in this disclosure allow for the identification, selection, and arrangement of cells, blocks, and modules in large scale battery systems, such as electric vehicle battery systems. An example embodiment of the present invention allows for the identification of the cells, blocks, and modules with a unique identifier or associated parameter (e.g., internal resistance contributions or capacity). Additionally, to form a block, cells may be selected from a group of capacity-range sorting bins. Based on a parameter of the cell, a pseudo-number generator may be also be used to select cells from an available inventory of cells as maintained in a cell database based. Cells may also be placed in a block for uniform effective cell impedance and even cell aging while minimizing overall block capacity degradation caused by cycling. Block capacities may also be computed based on a known average temperature gradient during operation.

The invention discloses a method for producing oyster mushroom strains from birch chopsticks. The method comprises four steps of: performing pretreatment on the chopsticks, preparing a culture medium for the chopsticks, performing inoculation culture and transferring the strains. According to the method disclosed by the invention, by carrying out pretreatment on the birch chopsticks, on the one hand, harmful substances in the birch chopsticks can be fully decomposed, and on the other hand, convenience is brought to the insertion of the birch chopsticks into a mycelium mass during the inoculation. When a culture medium for the birch chopsticks is prepared, as birch contains a large number of nutrient components required for growth of oyster mushrooms, only sucrose, a compound fertilizer, wheat bran and corn flour need to be further added, therefore the components in a nutrient solution are greatly simplified and the preparation process is also trouble-saving and effort-saving. When the inoculation is performed, only the chopsticks need to be directly inserted into the mycelium mass for completion, the operation process is convenient, fast, effort-saving and labor-saving and the pollution rate is very low. Mycelia are fully grown all over the whole chopstick strains after culture, the cell ages after the inoculation are short and consistent, the strains are fast in growth speed, the mycelia are robust, and the strains are high in purity and strong in resistance to other fungi. The method disclosed by the invention further has the more prominent advantages of low production cost and very significant economic benefits.

The invention discloses a healthcare soft capsule containing elements, vitamins and vegetable polyketide synthase and used for controlling cardiovascular and cerebrovascular diseases, and belongs to the technical fields of the scientific preparation and production of healthcare soft capsules containing natural vegetable extracts and used for controlling the cardiovascular and cerebrovascular diseases. The healthcare soft capsule is composed of traditional Chinese medicinal fluid extract nanopowder, macro and trace element groups mixed nanopowder, vitamin families mixed nanopowder, pine needle series grape seed, ginkgo biloba and human embryo extract ferment mixed nanopowder, and Radix Puerariae, Aizoon Stonecrop Herb, burdock, Ixeris chinensis Nakai and selenium-and-germanium-rich Mythic Fungus and ginseng series extract ferment mixed nanopowder. The healthcare soft capsule can effectively and comprehensively supplement, adjust and balance elements in an antagonistic body, strongly remove free radicals, enhance the vitalities of blood vessels, cardiac muscles and brain cells, activate mitochondria, and restore and correct cell damages caused by cell ageing and diseases to effectively control the cardiovascular and cerebrovascular diseases, fully supplement ferments, realize oxygen waste discharge and build the body.

The invention provides ZIF-8 nanoparticles coated with an anti-aging drug and the application of the ZIF-8 nanoparticles, the ZIF-8 nanoparticles are used for coating the anti-aging drug, the anti-aging drug is loaded in the ZIF-8 nanoparticles, the function of inhibiting cell aging is achieved, and the main application scenarios include inhibition of cell aging and the like in the cell in-vitro expansion link. In the invention, the ZIF-8 nanoparticles not only play a role of a carrier, but also play a role in enhancing the stability of the anti-aging drug. Different from medicines in other systems, the ZIF-8 nanoparticles coated with the anti-aging medicines disclosed by the invention have the advantages that the scavenging capacity of the anti-aging medicines on free radicals is fully exerted, so that the anti-cell aging effect is realized.

The invention relates to an oral solution prepared by traditional Chinese medicine and capable of improving immunity. The oral solution is composed by, by weight, 42-75 parts of cordyceps sobolifera, 5-15 parts of astragalus mongholicus, 3-9 parts of lucid ganoderma, 8-20 parts of ginseng and 4-12 parts of lotus seeds. The oral solution prepared by traditional Chinese medicine and capable of improving immunity has functions of organism immune adjusting function improving, renal function enhancing, hematopoietic system function promoting, nourishing and strengthening, fatigue resisting, stress resisting, anoxia resisting, high temperature resisting, body metabolism accelerating and cell ageing delaying, can obviously improve immunity, and is free of side effect and convenient to take.

An object of the invention is a method of identifying an eventual modification of at least one biological parameter. The present invention relates essentially to a method of identifying an eventual modification of at least one biological parameter, comprising the compared proteomic and / or compared transcriptomic and / or compared genomic analysis: a) of living young living cells, b) of living aged living cells, c) at least one of these two classes of cells being used in a three-dimensional tissue model, enabling eventually identifying at least one biological parameter which is modified further to cell ageing. The invention comprises the use of this process for the screening of active principles.

The invention discloses a liquid strain fermentation culture medium, and belongs to the technical field of pleurotus eryngii cultivation. Chinese herb extracts capable of improving cold resistance, heat irritability resistance, immunity and bacterium-resisting capacity of the strain are scientifically compounded in culture mediums for liquid seeds, shaking flask seeds, seeding tank seeds and fermentation tank liquid strains in all stages, and meanwhile wood powder which has the feedstock adaptability and has the function of an antifoaming agent is scientifically compounded; manners of supplemental inoculation and segmentation variable temperature fermentation are adopted, and therefore the difference between the strain cell age and the multiplication algebra is shortened; and packaged chitosan with a special function is properly fed, and the pleurotus eryngii liquid strain with the thick and solid fermentative hyphae, high activity, small mycelium pellet diameter, large density and uniform distribution is cultured. The density of the mycelium pellets ranges from 4.5*10<5>piece / L to 5.5*10<5>piece / L, the diameter of the mycelium pellets ranges from 0.5mm to 0.7mm, the dry weight of the mycelium pellets ranges from 90g / L to 100g / L, the germination time ranges from 4 hours to 5 hours, and the exopolysaccharide ranges from 3.0g / L to 3.5g / L.

The invention discloses markers for identifying or assisting in identifying vascular cells and / or vascular cell aging degrees and applications of the markers. The invention firstly discloses the applications of IL13RA2, WIF1, PTGS1, POSTN, DES, ACTC1, SORBS2 and PEBP4 in preparation of products that identify or assist in identifying the vascular cells. The invention further discloses the application of FOXO3 in preparation of products for identifying or assisting in identifying the aging degrees of the different vascular cells. The markers provided by the invention play an important role in the screening and identification of the vascular cells, can evaluate vascular aging related diseases such as atherosclerosis, and can detect human vascular function and evaluate the degrees of human vascular aging, thereby providing important molecular indications for the research and intervention of the vascular aging related diseases, especially for the evaluation of human cardiovascular functionand disease diagnosis; and the markers have important application value.

The invention provides an anti-aging medicine D / A targeting at aging cells in a tissue microenvironment and application of the anti-aging medicine D / A. Dasatinib is combined with aspirin, and a very excellent effect in reducing or eliminating aging cells of a body can be achieved, so that the medicine can be used for eliminating damaged cells such as damaged cells in the tissue microenvironment after chemotherapy or radiation therapy, and can be also used for eliminating cells aged naturally along with ages, and thus the living time of the body can be prolonged.

The invention discloses a high-density liquid strain and a fermenting method thereof, and belongs to the technical field of culture of pleurotus eryngii. According to the preparation method, Chinese herbal medicine extracts which can improve the cold resistance, thermal stress, immunity and hybrid bacteria resistance of the strain are scientifically composited in the culture medium at each stage of liquid seeds, seeds in a shake flask, seeds in a seed tank, and liquid strain in a fermenting tank; meanwhile, sawdust which has the raw material adaptability and also has the function of a defoaming agent is scientifically composited; in addition, the mode of supplemental inoculating and sectional adjustable temperature fermenting is carried out, in order to reduce the cell age of the strain and the difference in the number of generation of proliferation; chitosan which is coated and has the special function is added by a flowing manner at a proper time so as to culture the liquid strain which is strong in fermenting hypha, high in activity, small in mycelium pellet diameter, large in density, and uniform to distribute; the density of the mycelium pellet is 4.5 to 5.5x105 units / L; the diameter of the mycelium pellet is 0.5 to 0.7m; the dry weight of the mycelium pellet is 90 to 100g / L; the germination costs 4 to 5 hours; the exopolysaccharide is 3.0 to 3.5g / L.

The invention discloses blackberry wine capable of delaying cell ageing and a production method of the blackberry wine. The method comprises the steps of carrying out pre-fermentation, carrying out post-fermentation, stopping fermentation, carrying out enzymolysis, carrying out cold treatment and sterilizing. According to the method disclosed by the invention, fresh fructus lycii and folium ginkgoare added into blackberry raw materials according to a certain ratio; the fermentation is carried out so that the content of fructus lycii polysaccharide and flavone in the blackberry wine is increased; meanwhile, the quantity of saccharomycetes is increased in the post-fermentation; the saccharomycetes is self-dissolved in the wine through the enzymolysis and ultrasonic treatment to release a lot of functional substances including yeast polysaccharide, polypeptide, amino acid, nucleotide and the like, so that the health-care function of the blackberry wine is greatly increased; especially, the cell ageing can be delayed. According to the technology disclosed by the invention, high-quality blackberry fruit wine, which has excellent color, flavor and taste and integrates nutrients and health care as a whole, can be obtained.

The invention provides a cosmetic. The cosmetic is mainly prepared from an epidermal stem cell extract and aloe micromolecular bioactive peptide. The provided human epidermal stem cell extract contains various kinds of life active matter, can revitalize skin cells, activate the self-healing function of human body, supplement and condition aged cells, activate cell functions, increase the number ofcells, improve the cell viability, improve the cell quality, improve the material transportation function of cytomembrane and material interchange capability, recover the normal physiological function of cells, and promote corium layer cells to secrete and synthesize functional molecules such as collagenous fiber, polysaccharide and glycoprotein. The provided aloe micromolecular polypeptide can obviously improve the recovery function of the human epidermal stem cell extract on damaged skin, and the function of delaying cell ageing.

The invention relates to the technical field of gene engineering and biological enzyme with an aim to provides a preparation method and application of microbial-source antioxidant protease. The amino acid sequence of the protease is as indicated in SEQ ID NO.1. The invention further the preparation method of the protease and the application of the protease in preparing drugs or health food used for inhibiting body aging and cell apoptosis. The protease is the important protease for oxidoreduction balance adjustment and plays an important role in biological oxidative stress and damage repair process, thereby being potential in application value in the anti-aging field. Through combination and regulation of factors of ASK1, NF-k and the like, body ageing and cell apoptosis are inhibited, cells can be subjected to damage repair and proliferation, cell ageing and cell apoptosis can be postponed, and the protease is one of important targets in tumor and cancer treatment; the protease is soluble in physical characteristic, natural activity is retained, and wide application prospect is achieved.

The invention discloses an aging cell model and a preparation method thereof. The preparation method of the aging cell model comprises the following steps of reducing the content or activity of multipotential stem cell ATF6, so as to obtain ATF6 deficiency multipotential stem cell; inducing the ATF6 dificiency multipotential stem cell, so as to obtain ATF6 deficiency mesenchymal stem cell, whereinthe ATF6 deficiency mesenchymal stem cell is the aging cell model; the multipotential stem cell is embryo stem cell or induced multipotential stem cell. The aging cell model has the advantages that the cell propagation ability is decreased, the recession speed is accelerated, the survival ability is decreased, the integration ability is decreased, and the protein homeostasis is unbalanced in thecell; the increasing of protein aggregate in the cell can be used for establishing an individualized medicine screening platform which is used for screening candidate (natural) compounds for regulating and maintaining adult stem cell protein homeostasis, and retarding cell ageing.

The invention relates to the technical field of tea drink, in particular to grapefruit and wax gourd-containing tea and a preparation method thereof. The grapefruit and wax gourd-containing tea is prepared from the following raw materials in parts by weight: 60-65 parts of wax gourd juice, 1-2 parts of rapeseed oil, 0.1-0.2 part of tea polyphenol, 1-2 parts of jasmine flower extract, 1-2 parts of eclipta extract, 1-2 parts of artemisia steam extract, 1-2 parts of selenium-rich bayberry juice, 3-6 parts of selenium-rich green tea powder and 36-40 parts of chelated grapefruit juice. The grapefruit and wax gourd-containing tea is rich in major elements such as calcium, iron and zinc which are needed by a human body, and is rich in trace elements such as selenium; furthermore, the grapefruit and wax gourd-containing tea also contains rich vitamins, fructose, tartaric acid and citric acid; the grapefruit and wax gourd-containing tea has a good effect of quenching thirst, is capable of refreshing and restoring consciousness as well as clearing away the heart-fire and reducing internal heat, has a remarkable effect for clearing dampness and dispelling cold as well as preventing cold, and has certain help for cell ageing resistance.