44 results about "Cell ageing" patented technology

The application relates to application of semaglutide in preparation of a medicine for treating or delaying ovarian aging, and through a natural aging mouse model, a cell aging model and transcriptome analysis, it is first proved that semaglutide can significantly increase the number of follicles and reduce the level of aging markers, and from a molecular level, it is revealed that the mechanism of realizing multi-target point delay of ovarian aging is that semaglutide realizes multi-target point delay of ovarian aging through regulating an adipocyte cytokine signal pathway, inhibiting excessive cell division and regulating an NF-kappa B signal pathway. As a marketed drug, semaglutide has clear safety, a long half-life and high patient compliance, and has a significant clinical conversion prospect. The application breaks through the limitation that existing hormone replacement therapy and assisted reproductive technology can only treat symptoms or solve the problem of fertility, provides a first drug intervention strategy which can fundamentally delay the process of follicle depletion, and fills the blank in the field of ovarian aging treatment.

The invention discloses application of ellagic acid in preparation of a medicine or a biological product for preventing or relieving ultraviolet radiation damage and aging, and belongs to the technical field of biological medicine and radiation protection. Experimental verification shows that ellagic acid with effective concentration can promote expression of antioxidant related genes, reduce expression of inflammation response genes and expression of apoptosis related genes, eliminate active oxygen and inhibit lipid peroxidation reaction, so that oxidative damage, inflammatory reaction and apoptosis caused by ultraviolet radiation are effectively relieved, and the anti-inflammatory effect of ellagic acid is improved. The stress tolerance of an organism is improved, and the aging health risk induced by ultraviolet radiation is relieved; the medicine or biological product can be applied before and / or after exposure of ultraviolet radiation, and is suitable for preventing or relieving oxidative damage and cell aging caused by ultraviolet radiation. The invention provides a new way for developing safe and effective ultraviolet radiation protection and anti-aging preparations.

The invention discloses a nano vesicle with tissue targeting and mitochondrial localization as well as preparation and application of the nano vesicle. The nano-vesicle is a nucleus pulposus cell membrane entrapped with a small molecule mitochondrial membrane stabilizer, and tetra-acetylated N-acetamido mannose is added in the culture process of nucleus pulposus cells, so that a sialic acid derivative with an azide group is expressed on the surface of the nucleus pulposus cell membrane. Therefore, triphenylphosphine cations are modified on the surface of the nucleus pulposus cell membrane through a biological orthogonal reaction. The nano-vesicle can stabilize a mitochondrial membrane structure, improve cell aging-related secretion phenotype release and mitochondrial functions, block mtDNA release and SASP factor generation, realize anti-aging, anti-inflammatory and tissue degeneration intervention, can be used for preparing intervention drugs for tissue degeneration diseases related to mitochondrial membrane damage, and has broad application prospects. Or a drug for regulating mtDNA leakage and SASP release in a cell aging model. Wide application prospects and important clinical transformation values are realized.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with functions of resisting aging and whitening skin and application of the compound active peptide. Comprising acetyl hexapeptide-1, nonapeptide-1, acetyl tetrapeptide-11, hexapeptide-9 and palmitoyl hexapeptide-12. The compound peptide disclosed by the invention can play a synergistic anti-aging and whitening role on an epidermal layer and a corium layer, and has multiple advantages; the compound peptide acts on epidermal layer keratinocytes, dermal layer fibroblasts and B16F10 cells, and can be used for detecting the senescence of the cells by influencing the expression quantity of cell senescence marker genes, influencing the cell cycle, influencing the expression quantity of extracellular matrix related proteins, influencing melanin synthesis and influencing the secretion quantity of acetylcholine in the cells. Multiple skin anti-aging and whitening effects synergistically exerted on the epidermal layer and the corium layer are achieved.

The invention discloses an application of pseudo-ginsenoside RT5 in preparation of anti-cell and overall aging products. Pseudoginsenoside RT5 is protocatechol type saponin, and it is proved for the first time that pseudoginsenoside RT5 has remarkable anti-aging activity. In-vitro experiments show that the pseudoginsenoside RT5 relieves cell senescence through a multi-target mechanism (such as improvement of cell viability, reduction of SA-beta-gal and inhibition of SASP), and particularly, the average inhibition rate of the pseudoginsenoside RT5 to reactive oxygen species (ROS) reaches up to 62.9%. An in-vivo zebra fish experiment further verifies the overall anti-aging effect, and the SA-beta-gal staining index score in a D-galactose induced rapid aging model can be remarkably reduced. The pseudo-ginsenoside RT5 provided by the invention is derived from medicinal and edible plants, has the advantages of high safety and suitability for long-term use, and provides an efficient and multi-target acting natural active ingredient for preparing anti-aging products (such as foods, medicines, skin care products, cosmetics and the like).

The application provides an application of scEMC10 in treatment and diagnosis of brain vascular endothelial cell aging, and relates to the technical field of biomedicine. The inventor finds that scEMC10 deficiency leads to brain microvascular endothelial cell aging; brain microvascular endothelial cells treated by scEMC10 can down-regulate beta-galactosidase, SASP and classic aging markers related to aging; after old mice and 5XFAD model mice of Alzheimer's disease are supplemented with scEmc10, the movement coordination ability and spatial cognitive ability of the mice can be obviously improved, and the relative content of the Parkinson's disease marker, phosphorylated alpha-synuclein, of the old mice can be obviously reduced, and the content of the tyrosine hydroxylase phosphorylation activity form can be increased. Therefore, scEMC10 can be used for treatment of brain microvascular endothelial cell aging, improvement of Alzheimer's disease and Parkinson's disease, and detection of brain microvascular endothelial cell aging as a marker.

The invention discloses a pilose antler active peptide and application thereof in anti-inflammation, anti-oxidation and anti-aging, and belongs to the technical field of biology. According to the invention, the brand-new active peptide SPLASDLDL screened out from pilose antler for the first time is utilized, and competitive inhibition on TNFR1 is realized through the characteristic that the brand-new active peptide SPLASDLDL is specifically combined with a TNFR1 receptor. A constructed sertoli cell senescence model proves that the polypeptide has anti-inflammatory, anti-oxidation and anti-senescence effects. The invention successfully solves the technical problem that in the prior art, the competitive inhibition TNFR1 targeting peptide cannot be found from pilose antler and the action mechanism of the TNFR1 targeting peptide cannot be clarified, and provides the action mechanism and scientific basis for developing novel anti-inflammatory and anti-aging drugs derived from natural products.

The application relates to application of a reagent for detecting expression levels of a PSIP1 gene or a coded protein LEDGF / p75 in preparation of a product for evaluating systemic lupus erythematosus disease activity, which makes up for the problems of insufficient sensitivity and specificity of existing evaluation indexes; the application also discloses a key role of the PSIP1 gene in a pathogenesis mechanism of systemic lupus erythematosus, and clearly shows the relationship between the PSIP1 gene in T cells and cell cycle arrest, cell aging and abnormal immune function, so as to provide a theoretical basis for target regulation of the PSIP1 to improve T cell function and delay or block disease progression; the application firstly proposes that the expression levels of the PSIP1 / LEDGF / p75 are detected to be used for evaluating systemic lupus erythematosus disease activity, auxiliary diagnosis, prognosis prediction and treatment effect monitoring, so as to provide a brand-new tool and direction for precise diagnosis, activity evaluation, prognosis judgment and future target treatment development of systemic lupus erythematosus.

The invention discloses a Weissella sinus strain as well as a fermentation product filtrate and a biological product thereof, and belongs to the technical field of cosmetics. The strain can be used in the field of cosmetics, has excellent HAS inhibition capability, also has remarkable DPPH and ABTS free radical scavenging capability, and can realize a good antioxidant effect; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of various matrix metalloproteinases can be remarkably inhibited, and a certain effect of reducing cell senescence markers is achieved; in addition, the fermentation product filtrate can inhibit the activity of tyrosinase in vitro, can also significantly inhibit the generation of melanin in a zebra fish model, and has the potential of being used as a tyrosinase inhibitor or a melanin generation inhibitor; the fermentation product filtrate can also significantly inhibit the secretion amount of a cell inflammatory factor NO, has a good soothing effect, and has a certain ability to regulate the skin micro-ecology.

The invention discloses a plant lactobacillus for producing various active small molecules by fermenting truffles and resisting skin cell aging in multiple mechanisms, and belongs to the technical field of microorganisms. The lactobacillus plantarum CCFM1494 obtained by screening can be used for fermenting the white truffle, degrading cyanidin-3-O-glucoside chloride, and generating a plurality of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin, protocatechuic acid and the like; meanwhile, after the white truffle is fermented by the plant lactobacillus CCFM1494, the promotion effect of the white truffle on the synthesis of skin collagen and the improvement effect on cell senescence can be improved. Therefore, the lactobacillus plantarum CCFM1494 has a huge application prospect in preparation of products for improving skin quality.

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

The present invention provides a method of identifying the degree of aging of a subject or a tissue or organ thereof, comprising a) detecting the presence of an immunoglobulin (Ig) molecule in a sample from the subject; and b) comparing the result of the detection with a reference value. The invention also provides a method for regulating senescence of a subject or a tissue or organ or cell thereof through Ig, and a method for screening an anti-senescence compound or composition or identifying the effect of a drug on senescence by detecting Ig.

The invention discloses an application of SETD2 and NSD2 in diagnosis and treatment of aging of intestinal stem cells. Specifically, the invention relates to application of the intestinal stem cell aging marker or the detection reagent thereof, the intestinal stem cell aging marker or the detection reagent thereof is used for preparing a diagnostic reagent or a diagnostic kit, and the diagnostic reagent or the diagnostic kit is used for judging whether intestinal stem cells are aged or not. Wherein the senescence marker combination is prepared from SETD2 and NSD2 (National Solution Development 2). Meanwhile, the invention provides a detection reagent and a kit for the intestinal stem cell marker. The detection reagent or the kit can effectively judge the aging type of the intestinal stem cells, and different effective drugs are effectively used according to different types.

The invention discloses application of an extracellular matrix in preparation of an anti-vascular aging product, and belongs to the field of biological medicine. The acellular extracellular matrix provided by the invention is prepared by taking umbilical artery vascular smooth muscle cells as a material and carrying out water swelling and 0.5% Triton X-100 treatment on the umbilical artery vascular smooth muscle cells. The invention also provides application of the acellular matrix in preparation of drugs for inhibiting blood vessel and cell senescence. Experimental results show that after aortic vascular smooth muscle cells overexpressing Pelamin A are inoculated to the acellular matrix and cultured, the aortic vascular smooth muscle cells are distributed in a concentrated manner, the number of the aortic vascular smooth muscle cells is increased, the form of the aortic vascular smooth muscle cells is remarkably improved, the expression of X-GAL in the cells is reduced, and the expression quantity of Ki67 is increased; therefore, the acellular extracellular matrix provided by the invention can be used for effectively regulating aortic vascular smooth muscle cell senescence induced by Pelamin A accumulation. The invention provides a new biological material for inhibiting vascular aging and cell aging.

The invention discloses a targeted ATAD3A phosphorylated polypeptide and application thereof in preparation of an anti-aging product. The targeted ATAD3A phosphorylated polypeptide is composed of a functional peptide with an amino acid sequence as shown in SEQ ID No.1 and a cell-penetrating peptide connected with the N end of the functional peptide. Research finds that overexpression of phosphorylated ATAD3A promotes cell aging, which prompts that targeted inhibition of ATAD3A phosphorylation may have an anti-aging effect. Therefore, the invention designs the TAT-polypeptide targeting ATAD3A phosphorylation site for anti-aging treatment, and the TAT-polypeptide shows a good treatment effect in both cellular level and mouse anti-aging treatment. Therefore, the targeted ATAD3A phosphorylated polypeptide opens a new thought and provides a new target for exploring anti-aging treatment, and the polypeptide can be further used for preparing anti-aging drugs and relieving the pressure brought by population aging to social medical care.

The invention belongs to the technical field of cosmetics, and particularly discloses an anti-oxidation fucoidan composition for delaying skin stem cell aging and a preparation method and application of the anti-oxidation fucoidan composition. The antioxidant algin composition is prepared from the following components in parts by weight: 5 to 10 parts of algin extract, 5 to 10 parts of herba houttuyniae extract, 2 to 4 parts of black pine needle extract, 4 to 6 parts of sorbitan olivate, 4 to 6 parts of squalane, 5 to 10 parts of glycerol, 0.1 to 0.5 part of sodium hyaluronate, 0.1 to 0.5 part of xanthan gum, 0.1 to 1 part of ethylhexylglycerin and 0.1 to 1 part of p-hydroxyacetophenone. The invention discloses an antioxidant fucoxanthin composition for delaying skin stem cell aging and a preparation method and application thereof. The antioxidant fucoxanthin composition is high in antioxidant capacity and capable of effectively promoting collagen synthesis and delaying skin stem cell aging.

The invention relates to the field of bovine bones, in particular to a preparation method and application of anti-cell-aging and anti-oxidation bovine bone peptide. The method comprises the following steps: dissolving the bovine bone peptide in double distilled water until the final concentration is 200 mg / ml; the bovine bone peptide is red bovine bone peptide or yak bone peptide. And after the culture medium containing the bovine bone peptide component is used, the ROS level in the cells is obviously reduced to 1-1.5 times, and the ROS level has obvious difference. The invention discloses application of red cattle bone peptide or yak bone peptide in preparation of food or health care products or medicines for preventing cell aging. By adopting the technical scheme, compared with the prior art, the invention has the following beneficial effects: a novel evaluation method is provided, and an experimental method for the anti-aging effect of the bovine bone peptide and the yak bone peptide and related products are creatively found.

The invention discloses an active polypeptide OA-AL14, application and a preparation for preventing and treating skin photoaging, and belongs to the technical field of biological medicine. The active polypeptide OA-AL14 has the activity of preventing and treating skin photoaging, the amino acid sequence of the active polypeptide OA-AL14 is ALFWPMKKPWPESC, and the molecular weight of the active polypeptide OA-AL14 is 1720.06 Da; researches show that the active polypeptide has obvious ability to remove ABTS < + > free radicals and DPPH free radicals; in a UVB irradiation model test, the active polypeptide OA-AL14 shows the activity of promoting HaCaT cell proliferation and a cell protection effect, and can effectively relieve the oxidative stress reaction of the HaCaT cell induced by UVB and obviously improve the aging of the HaCaT cell induced by UVB; animal experiments further prove that the active polypeptide OA-AL14 can obviously improve UVB-induced skin photoaging, and has important value for development of novel skin photoaging prevention and treatment drugs.

The present disclosure generally relates to compositions and methods to increase resistance to age-related pathologies, improve physiological state or disorder related to cell ageing or metabolic fatigue in one or more cells; improve mobility and / or healthspan and / or lifespan of an adult animal in need. More specifically, the present disclosure relates to administering a formulation comprising an effective amount of nicotinamide (NAM) and Glycine, optionally an autophagy inducer.

The application discloses a compound, a radionuclide label of the compound and a prepared probe thereof, and belongs to the chemical field. 68 Ga-MGal can be used as a detection probe for recognizing beta-galactosidase, and is used for detecting the distribution of beta-galactosidase in a tissue organ. Those skilled in the art know that beta-galactosidase is a classic marker of cell aging, and therefore, the detection probe can judge the aging degree of the tissue organ by detecting the content of beta-galactosidase. Those skilled in the art also know that cell aging is also common in diseases such as cancer and fibrosis, and therefore, the detection probe can also judge the occurrence, development or prognosis state of the disease by detecting the content of beta-galactosidase.

The application relates to the technical field of pharmaceutical preparations, in particular to application of Thalia dealbata in inhibiting a PI3K / Akt / mTOR signal path to slow down animal cell aging, which finds that Thalia dealbata can delay body aging after being applied to an aging model, and can slow down the animal cell aging process by inhibiting the activity of the PI3K / Akt / mTOR signal path, and effectively improves the efficiency of Thalia dealbata in slowing down aging.

The application provides a kind of compound microsphere temperature-sensitive hydrogel for nasal administration and its preparation method and application, belong to the field of biological medicine technology.The application first researches and expounds that the increase of cell aging burden in hippocampus caused by anesthesia operation is the key reason of PND occurrence, and develops a kind of microfluidic hydrogel microsphere and cationic thermosensitive hydrogel compound for nasal PF4 delivery, by the way of combining methacrylated gelatin (GelMA) and methacrylated heparin (HepMA) microspheres (MS) loaded with PF4 with in-situ gelatinized chitosan (CS) temperature-sensitive hydrogel, a kind of compound gel nasal sustained-release preparation is prepared.The sustained-release preparation realizes sustained intracerebral drug delivery and cognitive recovery of PND mice, realizes the effect of minimally invasive, lower drug administration frequency and longer treatment duration.These findings reveal the key role of cell aging in PND, and propose that PF4-based therapy is a promising transformation strategy.

The present application relates to the field of microbial technology, and particularly relates to the application of AKK bacteria and products thereof in anti-aging and / or anti-oxidation. The preservation number of the AKK bacteria is CGMCC No. 20954, and the strain is preferably used in a pasteurized form. Through three kinds of zebrafish model experiments, it is proved that the strain can effectively improve cell aging, specifically, the telomere length is improved, the expression amount of anti-aging gene spns1 is enhanced, and the symptoms of muscle atrophy and cognitive impairment caused by aging are obviously improved. In particular, the minimum effective amount of the strain in the water body for feeding zebrafish is 250 μg / mL, and the minimum effective amount in the human body is 30 billion AFU per person per day. The present application provides a clear strain source and dosage basis for developing a multi-dimensional and systematic anti-aging functional composition.

The application discloses a new application of m6A modified gene GAS6 and its receptor MERTK in rheumatoid arthritis. Multi-omics integrated analysis of synovial tissue and peripheral blood mononuclear cells of RA patients reveals common dysregulation of transcriptomics and epitranscriptomics, highlighting genes with both differential expression and m6A modification, which are enriched in processes such as phagocytosis, Th17 differentiation and cell aging. Among these genes, GAS6 shows the most significant m6A hypermethylation and expression up-regulation, and is verified as a key effector molecule interacting with MERTK / AXL receptor. Functional experiments show that GAS6 and MERTK synergistically promote the malignant phenotype of RA fibroblast-like synoviocytes, enhance their proliferation, migration, inflammatory cytokine secretion and anti-apoptotic ability.

The invention discloses a medicinal and edible composition for delaying cognitive and memory function decline of old people, which comprises a fructus piperis longi extract, a radix asparagi extract and sodium hyaluronate, and is combined with highland barley, common turnip, maca, D-psicose and other active substances to prepare the medicinal and edible composition for delaying cognitive and memory function decline of old people. A series of medicinal and edible compositions for improving cognitive and memory function decline of old people are developed. The composition disclosed by the invention has a good protective effect in delaying senile cognitive and memory function decline, can be applied to senile cognitive and memory function decline accompanied by age increase, enhances the activity of telomerase, and improves neurotransmitter homeostasis and brain health. Molecular mechanism research shows that the compound can reverse senescence of neuronal cells of hippocampus by activating key targets in a phosphatidylinositol-3-kinase / protein kinase B signal channel. The composition can be used for being developed into food and health care products for improving attention, learning ability, language ability and judgment, has safety and effectiveness, and has a very good application prospect.

Method for delaying muscle loss, improving muscle endurance or delaying cell aging, comprising: administering to a subject in need an effective dose of the Limosilactobacillus reuteri and / or It's Metabolite. The Limosilactobacillus reuteri was deposited at Food Industry Research and Development Institute under the accession number BCRC911170 or erman Collection of Microorganisms and Cell Cultures under the accession number DSM34539.

The present invention relates to a polypeptide for delaying telomere shortening and its application, and belongs to the field of biotechnology. The present invention provides a polypeptide for delaying telomere shortening, and the amino acid sequence of the polypeptide is shown in SEQ ID NO.1. Studies have shown that the polypeptide has high biological activity and can significantly slow down the cell aging process by delaying telomere shortening, increasing the expression level of telomerase reverse transcriptase, increasing the activity of telomerase, and inhibiting the expression level of β-galactosidase. In addition, the polypeptide is derived from the fermentation process of natural dairy products and has high safety and low risk of side effects. In addition, the polypeptide has a small molecular weight and is easily absorbed by the human body, which can improve the bioavailability of the drug. Therefore, the polypeptide has great application prospects in delaying telomere shortening, anti-aging, and the treatment of diseases related to telomere loss or dysfunction.

The invention discloses a viral vector for accurately regulating and controlling a human telomerase gene by using a human interleukin-6 core promoter and application of the viral vector. The virus vector comprises an expression cassette with a human interleukin-6 core promoter sequence and a human telomerase gene sequence, and the human interleukin-6 core promoter sequence is activated in a natural physiological process of cell aging and inhibited in a normal cell physiological state; further accurately regulating and controlling the human telomerase gene to be expressed as telomerase protein; according to the invention, a human interleukin-6 core promoter and a human telomerase gene (hT) are respectively inserted into a virus vector to obtain the virus vector, and after the virus vector is transfected into human tissue cells, the aging of the cells can be delayed. According to the invention, the human interleukin-6 core promoter is utilized to dynamically and accurately regulate and control the expression of the human telomerase gene and construct the expression cassette and the virus vector, so that the expression cassette and the virus vector have a wide application prospect in the anti-aging and aging-related fields.

The invention provides a magnesium oxide-loaded asymmetric short fiber aerogel scaffold as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering materials. The preparation method comprises the following steps: adding MgO nanoparticles into a PLLA / Gelatin solution, and carrying out electrostatic spinning, crushing, freeze drying and crosslinking to obtain the porous short fiber aerogel scaffold (GP-coated MgO). The GP-coated MgO aerogel stent has a three-dimensional short fiber structure, and can release loaded MgO nanoparticles in a progressive and continuous manner while wound exudate is slowly absorbed. In-vivo and in-vitro experiments prove that the GP-coated MgO aerogel scaffold has biocompatibility and is very effective in the aspects of inhibiting inflammatory response and slowing down cell senescence. In addition, the GP-coated MgO significantly accelerates vascular reconstruction and epithelization, and promotes wound repair at the skin defect position of a diabetic rat.