Probiotic postbiotic composition for improving scalp environment and preparation method and application thereof

Abstract

The present invention discloses a probiotic postbiotic composition for improving the scalp environment, a preparation method thereof and an application thereof, wherein the probiotic postbiotic composition for improving the scalp environment comprises postbiotics obtained by fermenting two strains of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602; the Lactobacillus paracasei ProSci-101 has a preservation number of CGMCC No.26528; the Lactobacillus fermentum ProSci-602 has a preservation number of CGMCC NO.25451. The probiotic postbiotic composition for improving the scalp environment of the present invention has the effects of cell repair, enhancing elastin and renewing the metabolism of the dermis, can slow down cell aging, and promote the proliferation of stratum corneum cells; thereby effectively achieving the purpose of improving the scalp environment and preventing hair loss.

Description

Technical Field

[0001] The present invention relates to the technical field of washing and care products, and in particular to a probiotic and postbiotic composition for improving scalp environment, and a preparation method and application thereof. Background Art

[0002] The skin is like a barrier for the human body. It is soft, tough, and elastic. It protects the various tissues and organs in the body from damage by various harmful factors in the external environment. It also has natural moisturizing properties. However, with age, the skin's ability to retain water also weakens. Due to climate changes, seasonal changes, and bad living habits, the skin becomes dehydrated and dry, dandruff appears, and the stratum corneum is damaged. As for the scalp, most adults have different degrees of oily scalp, itchy scalp, dandruff, and even some have serious hair loss problems; therefore, it is necessary to repair the scalp environment.

[0003] Scalp microecology is a hot research topic in scalp health. Research on preventing and treating scalp diseases by regulating the balance of microecology is becoming more and more in-depth. Existing studies have found that when the balance of scalp ecology is broken, it will irritate the scalp and cause scalp diseases such as acne and dermatitis.

[0004] Postbiotics, as bacterial components or microbial metabolites of biologically active microorganisms, have been proven to be rich in a variety of organic acids, antimicrobial peptides, bacteriocins, short-chain fatty acids and other bioactive substances. A large number of experiments have shown that postbiotics play an important role in immune balance, digestive health and skin care. The use of postbiotics avoids the problem of low bioavailability and unstable effects of probiotics. However, the postbiotic solutions produced by the fermentation metabolism of various probiotics disclosed in the prior art are not significantly effective in improving the scalp cell environment and promoting cell metabolism and circulation after addition and use. It is usually necessary to compound other plant extracts with scalp care effects to effectively achieve the effect of improving the skin environment and moisturizing. Summary of the invention

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defect disclosed in the prior art that simply adding a probiotic postbiotic composition cannot significantly improve the scalp environment and prevent hair loss, thereby providing a probiotic postbiotic composition that can significantly improve the scalp environment and prevent hair loss when added alone, and providing a preparation method and application of the probiotic postbiotic composition.

[0006] A probiotic postbiotic composition for improving the scalp environment, characterized by comprising postbiotics obtained by fermentation of two strains of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602;

[0007] The Lactobacillus paracasei ProSci-101 has a preservation number of CGMCC No. 26528 and is recorded in the patent application with publication number CN116555112A.

[0008] The fermented Lactobacillus ProSci-602 has a preservation number of CGMCC NO.25451 and is recorded in the patent application with publication number CN115624517A.

[0009] The above-mentioned Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602 are both public strains, and their preservation information is not repeated here.

[0010] The postbiotic component is a mixture containing metabolites, which is rich in various organic acids, antimicrobial peptides, bacteriocins, short-chain fatty acids and other bioactive substances.

[0011] The postbiotics include antimicrobial substances, active substances and short-chain fatty acids.

[0012] In the postbiotics, the antibacterial substance is an organic acid component; preferably, the organic acid components include lactic acid 10-45 mg / g, citric acid 100-200 mg / g, oxalic acid 1.0-2.3 mg / g, malic acid 0.5-3.0 mg / g, phenyllactic acid 0.75-3.25 mg / g, succinic acid 0.3-3.5 mg / g, 4-hydroxyphenyllactic acid 0.2-3.8 mg / g, and phenylalanine 0.03-2.9 mg / g.

[0013] More preferably, the organic acid components also include salicylic acid 0.01-1.0 mg / g, vanillic acid 0.01-0.8 mg / g, benzoic acid 3-17 mg / g, and tartaric acid 0.01-1.2 mg / g.

[0014] And / or, in the postbiotics, the active substances include 1-5 mg / g of lauryl diethanolamine, 0.2-0.9 mg / g of soybean saponin, 0.05-2.25 mg / g of soybean isoflavones, 0.1-3.6 mg / g of γ-aminobutyric acid, 0.05-1.75 mg / g of malonyl genistin, 0.03-1.75 mg / g of D-psicose, 0.01-0.5 mg / g of 3-indoleacrylic acid, 0.01-0.5 mg / g of genistein, 0.03-0.5 mg / g of malonyl daidzein, 0.5-3.5 mg / g of irone, and Preaustinoid A 0.2-1.6mg / g, hyaluronic acid 0.02-1.85mg / g, extracellular polysaccharide 0.1-5.0mg / g, polypeptide 0.2-5.0mg / g; preferably, the active substance also includes isostearic acid 0.08-2.0mg / g, trihydroxyflavone 0.02-2.5mg / g, daidzein 0.01-2.4mg / g, and butyric acid glyceride 0.05-3.6mg / g.

[0015] And / or, in the postbiotics, the short-chain fatty acids include 10-80 mg / g acetic acid, 3-25 mg / g propionic acid, 0.05-10 mg / g butyric acid, 0.01-5 mg / g valeric acid, and 0.5-12.0 mg / g caproic acid.

[0016] The contents of the specific components of the above-mentioned antimicrobial substances, active substances and short-chain fatty acids are all calculated based on the mass of the postbiotics.

[0017] The probiotic-postbiotic composition for improving the scalp environment includes not only postbiotics, but also 11-16% of moisturizers, 4-9% of oils, 4-7% of skin conditioners, and 0.8-1.6% of emulsifiers, wherein the postbiotics account for 1.5-4.0% and the balance is deionized water.

[0018] If the amount of postbiotics added is less than 1.5%, the effect of compound use is not obvious and cannot be completely integrated with other components; if the amount of postbiotics added is greater than 4%, its compound use has a good effect of improving the scalp environment and preventing hair loss. At the same time, in hair care liquid, postbiotics also play the role of moisturizing and skin conditioning agents.

[0019] The moisturizing agent is at least one of glycerin, butylene glycol, allantoin, betaine, hydrolyzed sodium hyaluronate, gluconolactone, 1,3-propylene glycol and panthenol; more preferably, the moisturizing agent is one or more of glycerin, butylene glycol, betaine and hydrolyzed sodium hyaluronate.

[0020] Betaine has a strong hydrating effect. When applied to the skin surface, it can achieve the effect of hydrating and moisturizing, keeping the skin hydrated for a long time. It can also regulate the osmotic pressure of the scalp, promote the synthesis of protein in cells, and improve the cell vitality of scalp hair follicles.

[0021] And / or, the oil is at least one of squalane, jojoba oil, shea butter, borage oil, almond oil and linseed oil; further preferably, the oil is one or more of squalane, jojoba oil and linseed oil.

[0022] Squalane has a very high affinity and is the closest lipid to human sebum. When used on the skin, it can form a natural protective film for the skin. It also has good permeability and can reach the dermis through the skin, which can promote the renewal and proliferation of basal cells, repair damaged cells, delay cell aging, improve and enhance the cell's ability to retain water, and "treat both the symptoms and the root causes" to improve the scalp environment and enhance the growth of hair follicles.

[0023] Flaxseed oil contains a variety of unsaturated fatty acids and high-quality natural vitamins E and K, and has good permeability and affinity to the skin. When flaxseed oil enters the dermis of the skin, the fatty acids in it can be absorbed by the skin as nutrients, replenishing the lipid substances needed by the scalp and repairing the damaged stratum corneum. Flaxseed oil also forms a water-retaining film on the surface of the scalp to prevent moisture loss and dryness deep inside the skin.

[0024] And / or, the skin conditioning agent is at least one of p-hydroxyacetophenone, retinol, calendula extract, resurrection grass extract, pomegranate extract, and Aphanizomenon flos-water extract; further preferably, the skin conditioning agent includes retinol, calendula extract, and resurrection grass extract.

[0025] Retinol is a type of fat-soluble vitamin, namely vitamin A, which has a good protective effect on the scalp, promotes skin metabolism, accelerates skin keratinization, and prevents scalp dehydration and dryness and abnormal keratin metabolism. It also has the effects of anti-oxidation and promoting collagen regeneration, keeping the skin soft and plump, fundamentally strengthening the scalp's ability to lock in moisture, and preventing scalp dryness and aging;

[0026] Calendula extract can quickly activate dormant fibroblasts, promote cell division and self-repair and renewal, secrete collagen, enhance skin elasticity, enable continuous regeneration of skin cells, give the skin sufficient moisture and super strong water-locking ability, and promote cell self-repair, achieving a better effect of improving the head environment;

[0027] Resurrection grass extract is rich in glycosyl trehalose. This small molecule substance enters the skin base, replenishes cells with a large amount of moisture, revitalizes basal cells and makes cells younger.

[0028] And / or, the emulsifier is at least one of oleth-10, PEG-100 stearate, PEG-10 dimethicone, glyceryl oleate, polyglyceryl-6 polyricinoleate, cetearyl glucoside, sodium stearoyl lactylate, and sodium stearoyl glutamate.

[0029] In the probiotic postbiotic composition of the present invention, the oil constitutes the oil phase A, and the postbiotics and other ingredients other than the oil constitute the water phase B.

[0030] Furthermore, the oil phase A includes squalane, jojoba oil and linseed oil; the water phase B includes glycerin, butylene glycol, betaine, retinol, hydrolyzed sodium hyaluronate, calendula extract, resurrection grass extract, emulsifier and deionized water.

[0031] The preparation method of a probiotic postbiotic composition for improving the scalp environment comprises: mixing the other components of the probiotic postbiotic composition except the postbiotics to obtain a mixture and sterilizing the mixture, then adding the postbiotics to the mixture when the temperature is reduced to 42-50°C and mixing evenly.

[0032] Wherein, the preparation process of the mixture is:

[0033] Heat the oil to 80-85°C and stir evenly until it is completely dissolved and transparent to obtain oil phase A;

[0034] Mix the oil and other raw materials except the postbiotics, heat to 75-85°C and stir evenly until they are completely dissolved to obtain aqueous phase B;

[0035] Mix the oil phase A and the water phase B and continue stirring until the mixture is uniform. The temperature is controlled at 75-80℃.

[0036] After mixing evenly, a mixture is obtained;

[0037] The mixture was then sterilized by incubating at 75-80°C for 30 minutes.

[0038] The present invention also discloses the use of a probiotic-postbiotic composition for improving the scalp environment in products for improving the scalp environment and preventing hair loss; preferably, the product is a shampoo, a conditioner, a creamy hair mask or a hair care essential oil type cleaning and care product; the content of the postbiotic in the product is 1.5-4.0%.

[0039] The technical solution of the present invention has the following advantages:

[0040] 1. The probiotic postbiotic composition for improving the scalp environment provided by the present invention can restore the moisturizing ability of the scalp when added to a washing and care product, thereby achieving better effects of improving the scalp environment and preventing hair loss; specifically, the postbiotic ingredients selected by the present invention include Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602, and contain a variety of antibacterial substances and active ingredients, have the functions of cell repair, enhancing elastin and renewing the metabolism of the dermis, can slow down cell aging, and promote the proliferation of stratum corneum cells; the postbiotics in the present invention are obtained by fermenting two strains of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602, and compared with the postbiotic ingredients obtained by fermenting a single strain, the postbiotics have better effects of moisturizing, moisturizing, promoting hair growth and preventing hair loss, and can solve more scalp health problems caused by imbalance of scalp microecology.

[0041] 2. The probiotic postbiotic composition for improving the scalp environment provided by the present invention can also be compounded with a variety of other ingredients with multiple functions such as moisturizing, anti-allergic, and repairing to achieve the purpose of mutual synergy; in particular, the present invention uses marigold extract, resurrection grass extract, and postbiotic ingredients to compound, which can play a synergistic role. Through the use of multiple ingredients, the bioavailability of the postbiotic ingredients is improved, and the scalp environment is better improved. DETAILED DESCRIPTION

[0042] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.

[0043] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.

[0044] The preparation process of postbiotics obtained by fermentation of two strains of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602 is as follows:

[0045] Seed culture: 0.1 mL of the bacterial solution of Lactobacillus paracasei ProSci-101 was inoculated into MRS liquid medium (purchased from Beijing Luqiao Technology Co., Ltd.), and cultured at 30°C for 30 h to obtain ProSci-101 seed solution; 0.1 mL of the bacterial solution of Lactobacillus fermentum ProSci-602 was inoculated into MRS liquid medium, and cultured at 30°C for 30 h to obtain ProSci-602 seed solution;

[0046] Fermentation: ProSci-101 seed solution and ProSci-602 seed solution were inoculated into a fermentation medium until the total viable count of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602 in the fermentation medium reached 2×106 CFU / mL, and then cultured at 30°C for 30 hours to obtain a fermentation liquid; during the inoculation, the viable bacterial ratio of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602 was 1:1;

[0047] Post-treatment: sterilize the fermentation broth at 85°C for 15 min to obtain a sterilized fermentation broth; spray dry the sterilized fermentation broth to obtain postbiotics (the total number of cells of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602 in the postbiotics was 2.0×10 11 pcs / g);

[0048] Wherein, the preparation method of fermentation medium is as follows:

[0049] The fermentation medium consists of 1% defatted soy flour, 8% defatted milk powder and the balance water in percentage by mass; the components of the fermentation medium are mixed in proportion, and the mixture is stirred at 58° C. for 15 minutes (i.e., stirred at 150 rpm) to obtain a liquid; the liquid is homogenized once at 58° C. at a primary pressure of 19 MPa and a secondary pressure of 5.0 MPa to obtain a homogenized liquid; the homogenized liquid is sterilized at 93° C. for 30 minutes to obtain a sterilized liquid; the sterilized liquid is cooled to 35° C. to obtain a fermentation medium.

[0050] After testing, the postbiotics prepared by the above method include 22.2 mg / g lactic acid, 155.03 mg / g citric acid, 1.44 mg / g oxalic acid, 1.85 mg / g malic acid, 1.45 mg / g phenyllactic acid, 1.65 mg / g succinic acid, 1.03 mg / g 4-hydroxyphenyllactic acid, 0.38 mg / g phenylalanine, 0.075 mg / g salicylic acid, 0.053 mg / g vanillic acid, 7.2 mg / g benzoic acid, 0.055 mg / g tartaric acid. g, lauryl diethanolamine 3.03mg / g, soybean saponin 0.447mg / g, soybean isoflavone 0.282mg / g, γ-aminobutyric acid 0.438mg / g, malonyl genistin 0.265mg / g, D-psicose 0.241mg / g, 3-indoleacrylic acid 0.231mg / g, genistein 0.226mg / g, malonyl daidzein 0.212mg / g, irone 1.124mg / g, Preaustinoid A 0.481 mg / g, hyaluronic acid 0.065 mg / g, extracellular polysaccharide 0.303 mg / g, polypeptide 0.576 mg / g, isostearic acid 0.316 mg / g, trihydroxyflavone 0.298 mg / g, daidzein 0.283 mg / g, butyric acid glyceride 0.261 mg / g, acetic acid 32.82 mg / g, propionic acid 8.68 mg / g, butyric acid 2.14 mg / g, valeric acid 1.21 mg / g, caproic acid 4.69 mg / g.

[0051] Example 1

[0052] The probiotic and postbiotic composition for improving the scalp environment is also a hair care solution, and its specific composition is as follows:

[0053] Postbiotics: 1.5%;

[0054] Moisturizer: glycerin 3%, butylene glycol 4%, betaine 2%, hydrolyzed sodium hyaluronate 2%;

[0055] Oils: squalane 1%, jojoba oil 0.8%, linseed oil 2%;

[0056] Skin conditioning agents: retinol 3%, calendula extract 0.5%, resurrection grass extract 0.4%;

[0057] Emulsifier: 0.8%;

[0058] The balance was deionized water.

[0059] The preparation method of the above-mentioned probiotic postbiotic composition is:

[0060] (1) Mix squalane, jojoba oil and linseed oil in oil phase A, heat to 82° C. and stir evenly until completely dissolved and transparent;

[0061] (2) Mix the glycerin, butylene glycol, betaine, retinol, hydrolyzed sodium hyaluronate, calendula officinalis extract, resurrection herb extract, emulsifier and deionized water in the aqueous phase B, heat to 80° C. and stir evenly until completely dissolved;

[0062] (3) Mix the oil phase A and the water phase B and continue stirring until the mixture is uniform. The temperature is controlled at 78°C.

[0063] (4) After mixing evenly, keep the mixture warm for 30 minutes for sterilization, and then turn on the cooling water to cool down. The cooling water temperature is 25°C;

[0064] (5) When the temperature drops to 45°C, add the postbiotics and stir evenly;

[0065] (6) When the temperature continues to cool to 32° C., the material is discharged to complete the preparation of the hair care solution.

[0066] Example 2

[0067] The probiotic and postbiotic composition for improving the scalp environment is also a hair care solution, and its specific composition is as follows:

[0068] Postbiotics: 2.5%;

[0069] Moisturizer: glycerin 4%, butylene glycol 3.5%, betaine 3%, hydrolyzed sodium hyaluronate 2%;

[0070] Oils: squalane 1%, jojoba oil 0.8%, linseed oil 2%;

[0071] Skin conditioning agents: retinol 5%, calendula extract 0.5%, resurrection grass extract 0.7%;

[0072] Emulsifier: 0.8%;

[0073] The balance was deionized water.

[0074] The preparation method of the above-mentioned probiotic postbiotic composition is:

[0075] (1) Mix squalane, jojoba oil and linseed oil in oil phase A, heat to 82° C. and stir evenly until completely dissolved and transparent;

[0076] (2) Mix the glycerin, butylene glycol, betaine, retinol, hydrolyzed sodium hyaluronate, calendula officinalis extract, resurrection herb extract, emulsifier and deionized water in the aqueous phase B, heat to 80° C. and stir evenly until completely dissolved;

[0077] (3) Mix the oil phase A and the water phase B and continue stirring until the mixture is uniform. The temperature is controlled at 78°C.

[0078] (4) After mixing evenly, keep the mixture warm for 30 minutes for sterilization, and then turn on the cooling water to cool down. The cooling water temperature is 25°C;

[0079] (5) When the temperature drops to 45°C, add the postbiotics and stir evenly;

[0080] (6) When the temperature continues to cool to 32° C., the material is discharged to complete the preparation of the hair care solution.

[0081] Example 3

[0082] The probiotic and postbiotic composition for improving the scalp environment is also a hair care solution, and its specific composition is as follows:

[0083] Postbiotics: 4%;

[0084] Moisturizer: glycerin 4%, butylene glycol 5%, betaine 3.5%, hydrolyzed sodium hyaluronate 3%;

[0085] Oils: squalane 3%, jojoba oil 2%, linseed oil 4%;

[0086] Skin conditioning agents: retinol 5%, calendula extract 0.8%, resurrection grass extract 0.4%;

[0087] Emulsifier: 1.6%;

[0088] The balance was deionized water.

[0089] The preparation method of the above-mentioned probiotic postbiotic composition is:

[0090] (1) Mix squalane, jojoba oil and linseed oil in oil phase A, heat to 82° C. and stir evenly until completely dissolved and transparent;

[0091] (2) Mix the glycerin, butylene glycol, betaine, retinol, hydrolyzed sodium hyaluronate, calendula officinalis extract, resurrection herb extract, emulsifier and deionized water in the aqueous phase B, heat to 80° C. and stir evenly until completely dissolved;

[0092] (3) Mix the oil phase A and the water phase B and continue stirring until the mixture is uniform. The temperature is controlled at 78°C.

[0093] (4) After mixing evenly, keep the mixture warm for 30 minutes for sterilization, and then turn on the cooling water to cool down. The cooling water temperature is 25°C;

[0094] (5) When the temperature drops to 45°C, add the postbiotics and stir evenly;

[0095] (6) When the temperature continues to cool to 32° C., the material is discharged to complete the preparation of the hair care solution.

[0096] Example 4

[0097] A shampoo comprises 96% of a certain brand of shampoo without anti-hair loss effect on the market and 4% of a probiotic postbiotic composition for improving the scalp environment, wherein the probiotic postbiotic composition for improving the scalp environment contains only the postbiotics of the present invention.

[0098] The specific preparation method includes heating the shampoo without anti-hair loss function to 45°C, adding postbiotics, and stirring evenly.

[0099] Example 5

[0100] The only difference between this comparative example and Example 1 is that the skin conditioning agent in the probiotic postbiotic composition for improving the scalp environment is replaced by: 3% retinol and 1.2% calendula extract.

[0101] Example 6

[0102] The only difference between this comparative example and Example 1 is that the skin conditioner in the probiotic postbiotic composition for improving the scalp environment is replaced by: 3% retinol and 1.2% resurrection grass extract.

[0103] Comparative Example 1

[0104] The only difference between this comparative example and Example 1 is that no postbiotics are added to the probiotic postbiotic composition for improving the scalp environment.

[0105] Comparative Example 2

[0106] The only difference between this comparative example and Example 4 is that no postbiotics are added to the shampoo.

[0107] Experimental example

[0108] Test method: A total of 128 volunteers aged 25-55 were selected and randomly divided into 8 groups, each with 16 people, 8 men and 8 women. The volunteers in the same group were treated with the same product of the embodiment or comparative example.

[0109] Six groups of volunteers took 2 ml of the hair care liquid of Examples 1-3, 5-6 and Comparative Example 1 and applied it to their scalps every morning and evening, and massaged for 3-5 minutes until it was completely absorbed. The remaining two groups of volunteers took 2 ml of the shampoo of Example 4 and Comparative Example 2 and applied it to their scalps every day, massaged for 3-5 minutes, and then washed.

[0110] Every two weeks, the hair was combed, the fallen hair was collected and counted, and the growth of the hair was filmed with a micro camera to evaluate the hair density. Finally, the average integer value of each group was taken. The test results are shown in Table 1. The water content of the scalp stratum corneum was tested with a skin moisture tester Corneometer CM825, and the transepidermal water loss rate was measured with a Tewameter TM300 instrument. The test was performed three times and the average value was taken. The test site was the top of the head. The test results are shown in Table 2.

[0111] Sit still for 30 minutes in an indoor environment at 20°C and 50% relative humidity. Before applying, use a probe to test the scalp moisture content and moisture loss rate of the top test area of ​​the head. Measure three times and finally take the average of all the data as the data before applying. Apply the composition sample of the embodiment or comparative example on the test area at an application amount of 2 mg / cm 2 , measure the moisture content after 30 minutes and 1 hour respectively. The same measurement was made three times and the average value was taken to obtain the moisture content data. It was used continuously for sixteen weeks, and skin tests were carried out in the eighth and sixteenth weeks respectively. The results are detailed in Tables 3 and 4.

[0112] Table 1 Average number of hair loss (roots)

[0113]

[0114] As shown in Table 1, after using the hair care solutions of Examples 1, 2, and 3 for 16 weeks, the amount of hair loss of the volunteers was significantly reduced compared with before use (initial), and the amount of hair loss was significantly reduced after 16 weeks of use compared with Comparative Example 1. At the same time, the amount of hair loss in Example 4 was also significantly improved compared with Comparative Example 2, indicating that the probiotic postbiotic composition of the present invention has a certain anti-hair loss effect.

[0115] By comparing Example 1 with Examples 5-6 and Comparative Example 1, it can be seen that the present invention uses a combination of marigold extract, resurrection herb extract and postbiotic ingredients to achieve a synergistic effect of promoting anti-hair loss.

[0116] Table 2 Hair density (%)

[0117]

[0118]

[0119] As shown in Table 2, after using the hair care solutions of Examples 1, 2, and 3 for 16 weeks, the hair density of the volunteers was significantly increased compared with that before use (initial), and the hair density was significantly increased after 16 weeks of use compared with Comparative Example 1. At the same time, Example 4 was also significantly improved compared with Comparative Example 2, indicating that the probiotic postbiotic composition of the present invention has a certain hair growth effect.

[0120] By comparing Example 1 with Examples 5-6 and Comparative Example 1, it can be seen that the present invention uses a combination of marigold extract, resurrection herb extract and postbiotic ingredients to achieve a synergistic effect of promoting hair growth.

[0121] Table 3 Skin moisture content

[0122]

[0123] Table 4 Skin moisture loss rate (%)

[0124]

[0125] It can be seen from the above results that the hydrating and moisturizing probiotic postbiotic compositions prepared in the above embodiments and comparative examples of the present invention have good hydrating and moisturizing effects, and all volunteers had no allergic reactions or other discomfort reactions.

[0126] It can be seen from Examples 1, 2, 3, and 4 that the moisture content of the scalp stratum corneum increases with the increase in the amount of postbiotics added after 16 weeks of using the composition, and the moisture loss rate decreases accordingly. From the results of Example 1 and Comparative Example 1, it can be seen that the change rate of the 16w skin moisture content of Example 1 is 31.74%, and the change rate of the moisture loss rate is -26.14%, while Comparative Example 1 is 30.31% and -25.01% respectively; the change rate of the 16w skin moisture content of Example 4 is 32.92%, and the change rate of the moisture loss rate is -27.40%, while Comparative Example 2 is 28.35% and -25.39% respectively, indicating that the addition of postbiotics better improves the moisturizing effect of the composition, and is more helpful to improve the moisturizing performance of the scalp. The reason may be that the selected postbiotic ingredients have good ion resistance and are suitable for a wide pH range. A small amount of low-cost ingredients can be added for compound use to achieve a better moisturizing effect.

[0127] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. Application of a probiotic postbiotic composition in the preparation of a product for improving scalp environment and preventing hair loss, characterized in that: The probiotic postbiotic composition includes postbiotics obtained by fermentation of two strains of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602; The Lactobacillus paracasei ProSci-101 has a preservation number of CGMCC No.26528; The fermented Lactobacillus ProSci-602 has a preservation number of CGMCC NO.25451; The postbiotics include antimicrobial substances, active substances and short-chain fatty acids; The antibacterial substance is an organic acid component; the organic acid component includes lactic acid 10-45 mg / g, citric acid 100-200 mg / g, oxalic acid 1.0-2.3 mg / g, malic acid 0.5-3.0 mg / g, phenyllactic acid 0.75-3.25 mg / g, succinic acid 0.3-3.5 mg / g, 4-hydroxyphenyllactic acid 0.2-3.8 mg / g, and phenylalanine 0.03-2.9 mg / g; The active substances include 1-5 mg / g of lauryl diethanolamine, 0.2-0.9 mg / g of soybean saponin, 0.05-2.25 mg / g of soybean isoflavones, 0.1-3.6 mg / g of γ-aminobutyric acid, 0.05-1.75 mg / g of malonyl genistin, 0.03-1.75 mg / g of D-psicose, 0.01-0.5 mg / g of 3-indoleacrylic acid, 0.01-0.5 mg / g of genistein, 0.03-0.5 mg / g of malonyl daidzein, 0.5-3.5 mg / g of irone, 0.2-1.6 mg / g of Preaustinoid A, 0.02-1.85 mg / g of hyaluronic acid, 0.1-5.0 mg / g of exopolysaccharide, and 0.2-5.0 mg / g of polypeptide; The short-chain fatty acids include acetic acid 10-80 mg / g, propionic acid 3-25 mg / g, butyric acid 0.05-10 mg / g, valeric acid 0.01-5 mg / g, and caproic acid 0.5-12.0 mg / g.

2. The use according to claim 1, characterized in that: The probiotic postbiotic composition also includes 11-16% moisturizer, 3-9% oil, 3-7% skin conditioner, 0.8-1.6% emulsifier, 1.5-4.0% postbiotic, and the balance is deionized water.

3. The use according to claim 2, characterized in that: The moisturizing agent is at least one of glycerin, butylene glycol, allantoin, betaine, hydrolyzed sodium hyaluronate, gluconolactone, 1,3-propylene glycol and panthenol; and / or, the oil is at least one of squalane, jojoba oil, shea butter, borage oil, almond oil and linseed oil; and / or, the skin conditioning agent is at least one of p-hydroxyacetophenone, retinol, calendula officinalis extract, resurrection grass extract, pomegranate extract, and Aphanizomenon flos-aquae extract; And / or, the emulsifier is at least one of oleth-10, PEG-100 stearate, PEG-10 dimethicone, glyceryl oleate, polyglyceryl-6 polyricinoleate, cetearyl glucoside, sodium stearoyl lactylate, and sodium stearoyl glutamate.

4. The use according to claim 2 or 3, characterized in that: The oil constitutes an oil phase A, and the postbiotics and other ingredients other than the oil constitute an aqueous phase B.

5. The use according to claim 4, characterized in that: The oil phase A includes squalane, jojoba oil and linseed oil; The aqueous phase B includes glycerin, butylene glycol, betaine, retinol, hydrolyzed sodium hyaluronate, calendula officinalis extract, resurrection grass extract, emulsifier and deionized water.

6. The use according to claim 1, characterized in that: The active substance also includes 0.08-2.0 mg / g of isostearic acid, 0.02-2.5 mg / g of trihydroxyflavone, 0.01-2.4 mg / g of daidzein, and 0.05-3.6 mg / g of butyric acid glyceride.

7. The use according to claim 1, characterized in that The organic acid components also include salicylic acid 0.01-1.0 mg / g, vanillic acid 0.01-0.8 mg / g, benzoic acid 3-17 mg / g, and tartaric acid 0.01-1.2 mg / g.

8. The use according to claim 2, characterized in that: The preparation method of the probiotic postbiotic composition comprises: mixing the other components of the probiotic postbiotic composition except the postbiotics to obtain a mixture, sterilizing the mixture, and then adding the postbiotics to the mixture when the temperature is reduced to 42-50° C. and mixing the mixture evenly.

9. The use according to claim 8, characterized in that: The preparation process of the mixture is: Heat the oil to 80-85°C and stir evenly until it is completely dissolved and transparent to obtain oil phase A; Mix the oil and other raw materials except the postbiotics, heat to 75-85°C and stir evenly until they are completely dissolved to obtain aqueous phase B; Mix the oil phase A and the water phase B and continue stirring until the mixture is uniform. The temperature is controlled at 75-80℃. After mixing evenly, a mixture is obtained; The mixture was then sterilized by incubating at 75-80°C for 30 minutes.

10. The use according to claim 1, characterized in that: The product is a shampoo, a conditioner, a creamy hair mask or a hair care essential oil type cleaning and care product; the content of postbiotics in the product is 1.5-4.0%.

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