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Novel biomarkers for detecting senescent cells

A senescent cell and cell-free technology, which is applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of unsatisfied and missing detection of micro-innovative biomarkers and diagnostic methods for senescent cells

Pending Publication Date: 2020-06-30
UNIV FUR BODENKULTUR WIEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is a major disadvantage, as the analysis is limited to a specific tissue and may miss ongoing roles in other tissues
Furthermore, the use of biomarkers to specifically differentiate senescent from non-senescent cells in human tissues remains a controversial issue
Therefore, there is an unmet need for novel novel biomarkers and diagnostics to detect senescent cells

Method used

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  • Novel biomarkers for detecting senescent cells
  • Novel biomarkers for detecting senescent cells
  • Novel biomarkers for detecting senescent cells

Examples

Experimental program
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Effect test

Embodiment 1

[0273] 1. Materials and Methods

[0274] 1.1. Mice manipulation and serum sampling

[0275] p16-3MR (Demaria et al, 2014, Developmental Cell 31:722-733) mice were housed and housed at the AALAC-accredited Buck Institute for Research on Aging (Novato, CA) in the facility. 10 to 12 weeks old p16-3MR male mice were used in the experiment. The mice were divided into 4 groups with 3 mice in each group. In group A, control mice were injected with PBS. In group B, mice were treated with ganciclovir (GCV) (Sigma-Aldrich) in PBS for 5 consecutive days. In group C, mice were treated with doxorubicin hydrochloride (Sigma-Aldrich) in PBS. In group D, mice were first treated with doxorubicin and then GCV 10 days later. Mice were intraperitoneally injected with 10 mg / kg doxorubicin hydrochloride, and injected with GCV dissolved in PBS at a concentration of 25 mg / kg every day for 5 consecutive days. Four days after the last GCV administration, animals were euthanized with CO2 and bled...

Embodiment 2

[0307] 1. Materials and Methods

[0308] 1.1. Mice manipulation and serum sampling

[0309] p16-3MR (Demaria et al, 2014, Developmental Cell 31:722-733) mice were housed and maintained in the animal facility of the AALAC-accredited Buck Institute on Aging (Novato, CA). 10 to 12 weeks old p16-3MR male mice were used in the experiment. Mice were divided into 4 groups, 8 in each group, 5 females and 3 males. In group A, control mice were injected with PBS. In group B, mice were treated with ganciclovir (GCV) (Sigma-Aldrich) in PBS for 5 consecutive days. In group C, mice were treated with doxorubicin hydrochloride (Sigma-Aldrich) in PBS. In group D, mice were first treated with doxorubicin and then GCV 10 days later. Mice were intraperitoneally injected with 10 mg / kg doxorubicin hydrochloride, and injected with GCV dissolved in PBS at a concentration of 25 mg / kg every day for 5 consecutive days. 4 days after the last GCV administration, with CO 2 Animals were euthanized an...

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Abstract

The present invention provides a method of detecting senescent cells or diagnosing cellular senescence in a subject wherein the level of one or more selected mi RNAs is quantified in a sample from said subject.

Description

[0001] This invention was made with US Government support under NIH Grant No. P01-AG017242. The US Government has certain rights in this invention. [0002] The present invention provides a method for detecting senescent cells or diagnosing cellular senescence in a subject, specifically an in vitro method, wherein the level of one or more selected miRNAs in a sample from said subject to quantify. Background technique [0003] Cellular senescence is a stable form of cell cycle arrest, a mechanism that limits the proliferative potential of cells. Senescence responses can be triggered in multiple cell types in response to different cellular stresses. It is a potent barrier to tumorigenesis and contributes to the cytotoxicity of certain anticancer drugs. Although senescence limits tumorigenesis and tissue damage in a cell-autonomous manner, senescent cells can induce inflammation, tissue aging and destruction, and promote tumorigenesis and metastasis in a cell-nonautonomous man...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/136C12Q2600/158C12Q2600/178C12Q1/6851
Inventor J·格里拉里M·哈克尔J·坎皮西A·卡莱
Owner UNIV FUR BODENKULTUR WIEN
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