Preparation method for anti-ageing cell preparation

A cell preparation and anti-aging technology, applied in the direction of anti-virus agents, animal cells, vertebrate cells, etc., can solve problems such as limited understanding

Inactive Publication Date: 2016-12-07
ANHUI HUIEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aging and death are inevitable final outcomes for individuals of any species,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step (1), adding penicillin concentration to L-DMEM medium is 100U / mL, streptomycin concentration is 100U / mL, is mixed with bone marrow mesenchymal culture fluid;

[0025] Step (2), under aseptic conditions, rinse the bone marrow cavity of the rat with the bone marrow mesenchymal culture solution configured in step (1), add the washed out bone marrow into a sterile culture bottle, and add the step ( 1) The bone marrow mesenchymal culture solution prepared in 1), the volume ratio is 1:1, and the bone marrow cells are fully blown away with a sterile pipette to prepare a bone marrow cell suspension;

[0026] Step (3), centrifuging the bone marrow cell suspension obtained in step (2) at 800r / min for 5min, discarding the supernatant to obtain the bone marrow stem cell population;

[0027] Step (4), take the bone marrow stem cell population obtained in step (4) and add it to the culture medium prepared in step (1) to resuspend and filter it with a 70 μm sterile nylon filter, ...

Embodiment 2

[0032] Step (1), adding penicillin concentration to L-DMEM medium is 100U / mL, streptomycin concentration is 100U / mL, is mixed with bone marrow mesenchymal culture fluid;

[0033] Step (2), under aseptic conditions, rinse the bone marrow cavity of the rat with the bone marrow mesenchymal culture solution configured in step (1), add the washed out bone marrow into a sterile culture bottle, and add the step ( 1) The bone marrow mesenchymal culture solution prepared in 1), the volume ratio is 1:1, and the bone marrow cells are fully blown away with a sterile pipette to prepare a bone marrow cell suspension;

[0034] Step (3), centrifuging the bone marrow cell suspension obtained in step (2) at 1500r / min for 10min, discarding the supernatant to obtain the bone marrow stem cell population;

[0035] Step (4), take the bone marrow stem cell population obtained in step (4) and add it to the culture medium prepared in step (1) to resuspend and filter it with a 70 μm sterile nylon filter...

Embodiment 3

[0040] Step (1), adding penicillin concentration to L-DMEM medium is 100U / mL, streptomycin concentration is 100U / mL, is mixed with bone marrow mesenchymal culture fluid;

[0041] Step (2), under aseptic conditions, rinse the bone marrow cavity of the rat with the bone marrow mesenchymal culture solution configured in step (1), add the washed out bone marrow into a sterile culture bottle, and add the step ( 1) The bone marrow mesenchymal culture solution prepared in 1), the volume ratio is 1:1, and the bone marrow cells are fully blown away with a sterile pipette to prepare a bone marrow cell suspension;

[0042] Step (3), centrifuging the bone marrow cell suspension obtained in step (2) at 1200r / min for 7min, discarding the supernatant to obtain the bone marrow stem cell population;

[0043] Step (4), take the bone marrow stem cell population obtained in step (4) and add it to the culture medium prepared in step (1) to resuspend and filter it with a 70 μm sterile nylon filter,...

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PUM

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Abstract

The invention discloses a preparation method for anti-aging cell preparation. The preparation method includes the following steps: adding the extracted bone marrow into a culture fluid, centrifuging and discarding the supernatant and inoculating the culture medium in a culture dish; replacing the liquid for the first time after 24-48 hours of primary culture, then replacing the liquid every 3d, sub-culturing at the ratio of 1:2 in 12-18h after the third times of replacement, adding the third generation of cultured mesenchymal stem cells to the normal saline for resuspending, and preparing the bone marrow mesenchymal stem cell preparation. By separating and culturing the bone marrow mesenchymal stem cells, and adding the bone marrow mesenchymal stem cells cultured in vitro to the normal saline, the bone marrow mesenchymal stem cell preparation can be obtained; The result shows that the cell preparation can improve the damage of cell morphology of the aged rat, reverse aging damage to some extent, and provide clues for studying the anti-aging mechanism of the bone marrow mesenchymal stem cells, as well as human anti-aging.

Description

technical field [0001] The invention belongs to the technical field of cell preparations, in particular to a preparation method for anti-aging cell preparations. Background technique [0002] China is rapidly entering an aging society. At present, there are about 100 million Chinese people over the age of 10. It is estimated that in 2050, the elderly over 60 years old will account for 30% of the population in China, and the various troubles and problems brought about by aging will become more serious. Aging and death are unavoidable final outcomes for individuals of any species, but so far our understanding of the mechanism of aging is still quite limited. Given its importance, aging has always been a hot topic in biological research. [0003] Bone marrow mesenchymal stem cells are a type of pluripotent stem cells derived from the mesoderm in early embryonic development, and are stem cells of the connective tissue throughout the body. A large number of experiments have co...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K35/28A61P39/06
CPCC12N5/0663A61K35/28
Inventor 张正亮
Owner ANHUI HUIEN BIOTECH
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