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Method for screening anti-aging traditional Chinese medicine by virtue of telomere length

A technology of aging and traditional Chinese medicine, applied in the direction of biochemical equipment and methods, microbe measurement/inspection, etc., to slow down aging and realize the effect of modernization

Inactive Publication Date: 2013-06-12
CHANGZHOU ADAM BIOTECH
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AI Technical Summary

Problems solved by technology

Olovnikov speculated through experiments that the loss of base sequences caused by the duplication of telomeric DNA ends probably regulates the lifespan of cells

Method used

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  • Method for screening anti-aging traditional Chinese medicine by virtue of telomere length
  • Method for screening anti-aging traditional Chinese medicine by virtue of telomere length

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Experimental program
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Embodiment 1

[0017] (1) In vitro experiments

[0018] Specific implementation: WI-38 cells are seeded and cultivated in a 24-well cell culture plate containing 10% fetal calf serum MEM medium, 1×10 4 Cells / well, and 2.0 mmol L-1 glutamine, 1.0 mmol L-1 sodium pyruvate, 0.1 mmol L-1 non-essential amino acids, 1.5 g L-1 sodium bicarbonate were added to the culture medium. Usually the doubling time of young WI-38 cells is 12 h, and the cells enter the senescent state after continuous passage to 50 passages. Generally, when the cells are fully confluent on the 3rd to 4th day, the 1:4 passage is used, and the 1:2 passage is counted as one generation.

[0019] The cells were randomly divided into 4 groups: a young group, b blank control group, c simple t-BHP treatment group and d test drug pretreatment group.

[0020] young group : Culture starts from the 20th passage, and the cells are collected at the 30th passage.

[0021] Blank control group : Cells were cultured up to passage 38. ...

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Abstract

The invention relates to a traditional Chinese medicine screening method and particularly relates to a method for screening an anti-aging traditional Chinese medicine by virtue of telomere length. The method comprises the following steps of: accelerating the aging of a diploid fibroblast cell of a human embryo lung under the action of teri-butyl hydroperoxide so as to manufacture an aging cell model; after the model is manufactured, treating the cell by use of various selected traditional Chinese medicine condensed compounds; and after the treatment is finished, extracting the genome DNA (Deoxyribonucleic Acid) of the cell, detecting the telomere length, and judging whether the traditional Chinese medicine is a febrile medicament, namely having anti-aging activity according to the result.

Description

[0001] Technical Field The present invention relates to a method for screening traditional Chinese medicines, in particular to a method for screening anti-aging Chinese medicines by telomere length. Background technique [0002] The application of traditional Chinese medicine has a history of thousands of years in our country, providing a strong guarantee for the reproduction and continuation of the Chinese nation, especially in the treatment of critical and difficult diseases, showing unique advantages. With the rise of the global natural medicine trend, herbal medicine and traditional medicine have obtained unprecedented development opportunities. In 2000, the global natural herbal medicine market sales reached 16.5 billion US dollars, and the growth rate has remained at 10% to 15% in recent years. Yunnan is known as the "Kingdom of Animals and Plants". According to survey statistics, there are 4,758 kinds of medicinal plants, 260 kinds of medicinal animals, and 32 kinds of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 叶亚东路易斯伊格纳罗张娟全利郝小江朱兆云滕凌朱文华胡娟肖慧潘鹂张俊杨紫俊
Owner CHANGZHOU ADAM BIOTECH
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