40 results about "Telomeric dna" patented technology

The subject invention pertains to methods for rapid and accurate determination of subtelomere DNA sequences. Also provided are kits for determination of subtelomere sequences and uses of chromosomal terminal sequences for studying pathogenesis and treatment of diseases.

A category of γPNA miniprobes and chimeric γPNA probes is especially useful for detecting RNA and telomeric DNA in a cell sample. In particular, the probes can be used to deliver fluorescent dyes to the telomeres, allowing direct visualization of telomeres in cells.

The invention provides a rapid method for measuring the length of chromosome telomere by a flow cytometry. Operation of performing lucifugal ice bath is increased before the conventional denatured hybridization step, a telomere probe has sufficient time to enter a cell and can be hybridized with telomere DNA rapidly and effectively after the DNA is deformed, so that the hybridization efficiency is improved and the hybridization success rate is increased; 1 percent paraformaldehyde serves as a fixing liquid, so the fixing effect is better and more stable, and the use is facilitated; 70 percent deionized formaldehyde is added into a hybridization buffer solution, and the stability of the cell structure and the DNA can be protected when probe hybridization is conducted for a long time, so that the hybridization success rate is increased. Through the improvement, compared with the traditional method for measuring the length of the chromosome telomere, the method is quicker and more accurate, and is suitable for long-term and large-scale research work.

The invention provides a human chromosome telomere length rapid quantitative detection method and a kit thereof. The method is characterized in that the chromosomal telomere DNA length and changing rate are accurately measured. The basic principle is that the temperature of the chromosome telomere DNA is quickly changed from 98 DEG C to 50 DEG C, to achieve rapid cooling of the temperature and to achieve the purpose of hybridization of a liquid phase double-stranded probe and nucleic acid molecules. By detecting the fluorescence value before and after the hybridization, the fluorescence change value is calculated and converted to the fluorescence change amount corresponding to the unit DNA to be detected, that is, the change amount of the fluorescence value detected before and after the hybridization respectively is divided by the amount of the DNA for the hybridization, to indicate the relative length of the telomere DNA. According to the method, the hybridization temperature and temperature dropping time are strictly controlled; the renaturation of the telomere DNA is minimized, so that the telomere DNA is more fully hybridized with the probe. Therefore, the method has the advantages of being accurate, fast, cheap, convenient, efficient, time-saving and effort-saving, and providing a simple and practical technical method for the telomere DNA length detection.

The invention discloses a quinnazolidone derivative, a preparation method for the same and application thereof. As shown in the following constitutional formula of the quinnazolidone derivative, R1 refers to F, Cl, Br or I, R2 refers to NH(CH2)nNR5, NR5 or NH(CH2)nAr, R3 refers to Ar, R4 refers to NHCO(CH2)nNR5 or CONH(CH2)nNR5, Ar stands for various aromatic nucleuses, n is equal to 1, 2, 3, 4, or 5, and R5 refers to C1-6 alkyl, C3-6 naphthenic base, piperidyl, morpholinyl, piperazine or oxazoline. The quinnazolidone derivative has quite high inhibitory action on protooncogene DNA (deoxyribose nucleic acid) such as telomeric DNA, c-kit and the like, and has remarkable inhibitory action on various cancer cell strains, is small in toxicity to normal cells, and has a wide application space for preparation of anti-cancer drugs.

The invention provides a peptidyl-substituted double-chain benzofuran quinoline derivative. The structural formula of the peptidyl-substituted double-chain benzofuran quinoline derivative is shown in formula (I). In the formula (I), R1 is dipeptide, tripeptide, tetrapeptide and pentapeptide. Experiments prove that the peptidyl-substituted double-chain benzofuran quinoline derivative provided by the invention has very strong inhibition effects on expression of proto-oncogene DNA (deoxyribonucleic acid) such as telomere DNA, c-myc and the like, has significant inhibition effects on various cancer cell lines, has low toxicity to normal cells, is a peptide chain substituted benzofuran quinoline compound with low toxicity and good anti-cancer effects, and has wide application spaces in preparation of anti-cancer medicaments.

The invention relates to the field of medicinal chemistry, in particular to a 2-phenyl quinazoline derivative. The 2-phenyl quinazoline derivative has the structural formula in the figure, wherein R1 is NH(CH2)3N(CH3)2; R2 is C1 or H; R3 is NHCO(CH2)nNR4 or NHCO(CH2)nNH(CH2)nNR4; n is equal to 1, 2, 3, 4 or 5; NR4 represents two C1-6 alkyls on an N atom, two C3-6 naphthenic bases or NR4 represents morpholinyl, piperazinyl or pyrrolidinyl. The invention further discloses a preparation of the 2-phenyl quinazoline derivative. The 2-phenyl quinazoline derivative has strong inhibition to telomere DNA expression, can significantly inhibit multiple kinds of cancer cell lines, has small toxicity to normal cells, and can be widely applied to preparation of anti-cancer drugs.

The invention belongs to the technical field of biological detection sensors, and discloses a hydrogen sulfide colorimetric sensor based on a G4-Cu<2+> mimic enzyme system. In MES (Morpholineethanesulfonic acid) buffer solution, independent Cu<2+> does not have an obvious catalytic function on H2O2 oxidation TMB (tetramethylbenzidine); after human telomere DNA (deoxyribonucleic acid) is added, human telomere can form a G-quadruplex, and meanwhile, the human telomere can be combined with Cu<2+> to form a G4-Cu<2+> mimic enzyme compound, and catalytic capacity is greatly improved; after H2S is met, Cu<2+> and H2S have good specificity, and CuS precipitates are generated so as to lower the enzyme catalytic capacity of G4-Cu<2+>; and along with the increasing of H2S concentration, TMB color development is gradually changed into colourless from blue. By use of the sensor, the color change of the system of the sensor can be used for qualitatively and quantitatively detecting H2S. Due to theadding of the G-quadruplex, the detection sensitivity of a sensing system for H2S is improved, the detection limit of 7.5nM is realized, and the sensing system has good selectivity for H2S.

The invention relates to a method for screening G-quadruplex ligands on the basis of photoinduced electron transfer. The method comprises the following steps: adding a certain amount of traditional Chinese medicine monomer solution into a diluted hairpin DNA solution, and detecting the change of the fluorescence strength of the diluted hairpin DNA solution before and after the traditional Chinese medicine monomer solution is into the diluted hairpin DNA solution, thus realizing the screening of G-quadruplex ligands. The method fully utilizes the mechanism that G-quadruplex ligands can induce a hairpin probe containing a human telomerase DNA fragment to form G-quadruplex, so that labeled carboxy fluorescein (FAM) on the hairpin probe piles on a G-tetrad plane consisting of four G-bases and is close to the G-bases to be subjected to photoinduced electron transfer, and the fluorescence strength is reduced. The method has the advantages of simplicity in operation, high response speed, high universality and easiness in popularization, can realize high-flux screening of G-quadruplex ligands, and provides a simple and effective way for screening antitumor drugs targeting G-quadruplex ligands.

The invention provides a telomerase activity testing system based on an SPR technology. The telomerase activity testing system comprises a kit containing a buffer solution of 10-200 mmol/L and dNTP of 8-12 mmol/L, and an SPR chip provided with a glass sheet, a metal coating film covering the glass sheet and a telomerase DNA connected to the coating film, wherein the pH of the buffer solution is 7.3. Signal sources such as radioactive isotope or fluorescent markers are not needed, the safety is improved, and the detection costs are greatly reduced. The influence of PCR experiments is eliminated, further detection is achieved, and the experiment process is simplified. Separation and detection conducted on an amplification product are not needed, the detection speed is greatly improved, the detection real-timeliness and repeatability can be achieved, and the experiment reliability is improved.