Telomerase inhibitor screening system based on SPR technology

A technology of telomerase inhibitor and telomerase, which is applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of high cost, long time, heavy workload, etc., and achieve the goal of speeding up the screening and development work Effect

Inactive Publication Date: 2017-02-22
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method has the disadvantages of heavy workload, long time and high cost

Method used

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  • Telomerase inhibitor screening system based on SPR technology
  • Telomerase inhibitor screening system based on SPR technology
  • Telomerase inhibitor screening system based on SPR technology

Examples

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Effect test

example 1

[0037] 1) the preparation of kit, it has first reaction solution, second reaction solution and diluent, described first reaction solution comprises the phosphate buffer of 10mmol / L and the dNTP of 100mmol / L, the pH of described buffer is 7.3; the second reaction solution comprises 100000U / ml of telomerase and a pH of 7.3 telomerase stock solution, and the telomerase stock solution comprises 10mmol / L PB buffer, 3mmol / L MgCl 2And 8wt% glycerol, the pH of the stable solution is 7.3; the diluent is a 10mmol / L PB buffer solution with a pH of 7.3, which is used to separate the first reaction solution and the second reaction solution in the actual test process. Dilute to appropriate concentration of dNTP and telomerase test working solution;

[0038] 2) Construction of the chip: After coating a silver film with a thickness of 43 nm on the glass slide, place it in a 5 μmol / L solution of thiol-modified telomeric DNA and incubate at 0°C for 10 hours, and connect the telomere DNA to the ...

example 2

[0041] 1) the preparation of kit, it has first reaction solution, second reaction solution and diluent, described first reaction solution comprises the phosphate buffer of 200mmol / L and the dNTP of 120mmol / L, the pH of described buffer It is 7.3; the second reaction liquid comprises telomerase of 100000U / ml and pH is the telomerase stock solution of 7.3, and the telomerase stock solution comprises the MgCl of 200mmol / L PBS damping fluid, 15mmol / L 2 And 10wt% glycerol, the pH of the stable solution is 7.3; the diluent is a 200mmol / L PBS buffer solution with a pH of 7.3, which is used to separate the first reaction solution and the second reaction solution in the actual test process. Dilute to appropriate concentration of dNTP and telomerase test working solution;

[0042] 2) Construction of the chip: After plating a layer of gold film with a thickness of 46 nm on the glass slide, place it in 15 μmol / L telomeric DNA solution modified with trithioadamantane derivatives and incuba...

example 3

[0048] 1) the preparation of kit, it has first reaction solution, second reaction solution and diluent, described first reaction solution comprises the phosphate buffer of 100mmol / L and the dNTP of 110mmol / L, the pH of described buffer 7.3; the second reaction solution comprises 100000U / ml of telomerase and a pH of 7.3 telomerase stock solution, and the telomerase stock solution comprises 100mmol / L PB buffer, 10mmol / L MgCl 2 And 9wt% glycerol, the pH of the stable solution is 7.3; the diluent is a 100mmol / L PBS buffer solution with a pH of 7.3, which is used to separate the first reaction solution and the second reaction solution in the actual test process. Dilute to appropriate concentration of dNTP and telomerase test working solution;

[0049] 2) Construction of the chip: After plating a layer of gold film with a thickness of 44nm on the glass slide, place it in a 10 μmol / L solution of thiol-modified telomeric DNA and incubate at 5°C for 18 hours, and connect the telomeric ...

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Abstract

The invention provides a telomerase inhibitor screening system based on an SPR technology. The system comprises a kit and an SPR chip, wherein the kit is provided with a first reaction liquid, a second reaction liquid and a diluent, the first reaction liquid comprises 100-120 mmol / L dNTP and a dNTP stock solution, and the second reaction liquid comprises telomerase and a telomerase stock solution; the diluent is 10-200 mmol / L PB or a PBS buffer solution which has pH of 7.3; the SPR chip is provided with a glass plate, a metal coating film covering the glass plate and telomeric DNA connected with the coating film. The telomerase inhibitor screening system based on the SPR technology can achieve simple, fast, stable and reliable screening work of telomerase inhibitors through mature SPR equipment on the market.

Description

technical field [0001] The present invention relates to a screening system for telomerase inhibitors. More specifically, the present invention relates to a screening system for telomerase inhibitors based on SPR technology. Background technique [0002] Telomerase (telomerase) is a kind of reverse transcriptase, which is an RNA ribose-protein that can use telomere DNA as a substrate to repeat DNA amplification at the end of telomere DNA at the end of a eukaryotic linear chromosome Complex. The amplification of telomere DNA by telomerase can make cells divide indefinitely without natural apoptosis, thus leading to the occurrence of cancer. At present, overexpression of telomerase has been found in more than 85% of cancer cells. Studies have shown that inhibiting telomerase activity can kill cancer cells. Therefore, drug screening targeting telomerase has become an Research hotspots for treatment. Screening substances that can inhibit telomerase activity from a huge compou...

Claims

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Application Information

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IPC IPC(8): G01N21/552
CPCG01N21/553
Inventor 陈名利尹焕才田晶晶陆一泓
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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