Application of polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+>, and application method thereof

An application method and complex technology, applied in the field of application of polypyridine ruthenium complex [Ru2]2+, can solve the problem of less action

Inactive Publication Date: 2012-05-23
TONGJI UNIV
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Problems solved by technology

Although there are many studies on the recognition of double-stranded DNA by ruthenium polypyridine complexes, there are relatively few studies on their interaction with DNA with special structures, especially the recognition of the two important structures of G-quadruplex and i-motif Rarely reported

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  • Application of polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]&lt;2+&gt;, and application method thereof
  • Application of polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]&lt;2+&gt;, and application method thereof
  • Application of polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]&lt;2+&gt;, and application method thereof

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Embodiment Construction

[0026] 1. Preparation of buffer solution

[0027] Prepare the following buffer solution with high-purity water:

[0028] Buffer solution 1: 100mM KCl, 10mM Tris, pH=5.5;

[0029] Buffer solution 2: 100mM NaCl, 10mM Tris, pH=5.5;

[0030]2. Preparation of DNA stock solution

[0031] 1) Preparation of G-quadruplex DNA stock solution

[0032] Take a certain amount of G-rich single-stranded DNA (the nucleotide sequence is 5′-AGGGTTAGGGTTAGGGTTAGGG-3′), dissolve it in buffer solution 1 or buffer solution 2, seal and heat to 90° C., and keep it for 5 minutes. Then cool down to room temperature naturally, put it in a refrigerator at 4°C for 24 hours, and set aside. And measure the absorbance value A of DNA at 260nm with a UV spectrophotometer 260 . The molar extinction coefficient is 2.285×10 5 m 3 cm -1 , DNA concentration [DNA]=K×A 260 / 2.285×10 5 (k is the dilution factor), the unit is mol L -1 .

[0033] 2) Preparation of i-motifDNA stock solution

[0034] Take a ce...

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Abstract

The invention relates to an application of a polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+>, wherein the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> can be adopted as a molecular recognition reagent for recognizing a G-quadruplex structure and an i-motif structure of telomere DNA. According to the present invention, the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> provides different bonding capacities for the G-quadruplex structure and the i-motif structure of the telomere DNA, and the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> provides better bonding capacity for the G-quadruplex structure of the telomere DNA compared to the i-motif structure of the telomere DNA, such that the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> can be adopted as the good molecular recognition reagent, wherein the polypyridyl ruthenium complex [Ru(bpy)2(dppzi)]<2+> is respectively added to the DNA solution containing the G-quadruplex structure and the DNA solution containing the i-motif structure, a fluorescence titration method or an ultraviolet titration method is adopted, the change of the fluorescence spectrum or the ultraviolet absorption spectrum is observed, and the two important structures of the telomere DNA can be effectively recognized and distinguished.

Description

technical field [0001] The present invention relates to metal complexes, in particular to a polypyridine ruthenium complex [Ru(bpy) 2 (dppzi)] 2+ applications and methods of application. Background technique [0002] In recent years, the research on polypyridine ruthenium complexes is very active, which is because these complexes have unique DNA binding ability, good excited state activity, antitumor activity, rigid plane, good thermodynamic stability, rich photochemical and photophysical information, etc. Features [1]. These properties make such complexes have important application prospects in many fields such as molecular biology, medicine, and bioinorganic chemistry. Especially in the aspects of bonding with DNA, such as DNA structure probes, DNA molecular optical light, DNA footprinting reagents, DNA fragmentation reagents and anticancer drugs, etc. have very important applications [2,3]. [0003] In the 1930s and 1940s, Hermann Muller and Barbara McClintock simulta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 石硕赵娟姚天明
Owner TONGJI UNIV
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