32 results about "Ultraviolet absorption spectrum" patented technology

The invention relates to the technical field of leather wastewater treatment, and provides an ultraviolet-visible spectrum-based leather wastewater ozone dosage and COD (Chemical Oxygen Demand) prediction method. The method comprises the following steps: firstly, determining the types of organic matters in a tannery wastewater sample, and determining the distribution characteristics of the organic matters in different fluorescent regions through three-dimensional fluorescence spectrum analysis, so as to discriminate the types of organic pollutants; secondly, screening key wavelengths, and determining characteristic absorption wavelengths representing different organic matters through an ultraviolet-visible light spectrum; then carrying out an ozone oxidation experiment, and synchronously measuring a COD value and an ultraviolet absorption spectrum under different ozone addition amounts; based on experimental data, a multiple linear regression and exponential regression prediction model of ozone dosage and a COD prediction model based on partial least squares regression are established. According to the invention, accurate control of ozone dosage and real-time monitoring of COD are realized, the treatment efficiency is improved, the operation cost is reduced, the adaptability to water quality fluctuation is enhanced, and the method has a wide engineering application prospect.

The invention relates to the field of water quality detection, in particular to a water quality sampling detection method and device applied to a small and micro wetland unit. The method comprises the following steps: determining a turbidity coefficient according to scattered light intensity of a target water sample under different wavelengths, absorbance of a target interval in an ultraviolet absorption spectrum and fluorescence intensity of a target area in a fluorescence spectrum; determining a scattering equivalent absorption value of each wavelength according to the scattered light intensity of the target water sample under each wavelength, the absorbance corresponding to each wavelength in the ultraviolet absorption spectrum and the turbidity coefficient; determining a real ultraviolet absorption signal of each wavelength according to the absorbance corresponding to each wavelength in the ultraviolet absorption spectrum and the scattering equivalent absorption value of the wavelength; correcting the fluorescence spectrum according to the turbidity coefficient and the scattering equivalent absorption value of each wavelength to obtain a corrected fluorescence spectrum; and detecting the water quality according to the corrected fluorescence spectrum and the real ultraviolet absorption signal of each wavelength. According to the invention, accurate water quality sampling detection can be provided.

This invention discloses a method for detecting polycyclic aromatic hydrocarbons (PAHs) based on ultraviolet fluorescence and absorption spectroscopy, comprising: Step 1, acquiring the ultraviolet absorption spectrum and the fluorescence emission spectrum of a water sample under ultraviolet light excitation; Step 2, analyzing the corresponding CDOM fluorescence spectrum using the ultraviolet absorption spectrum obtained in Step 1; Step 3, subtracting the CDOM fluorescence spectrum obtained in Step 2 from the fluorescence emission spectrum obtained in Step 1 to obtain a mixed fluorescence spectrum containing multiple PAHs; Step 4, performing multivariate fitting on the mixed fluorescence spectrum obtained in Step 3 to obtain the type and concentration of each PAH in the water sample. This invention also provides a PAH detection device. The method provided by this invention is simple to operate and can directly detect raw water samples, thereby obtaining more accurate PAH detection results.

The invention provides an intelligent control method for cyclic utilization of washing water, which comprises the following steps: collecting multi-mode water quality data such as ultraviolet absorption spectrum, optical scattering intensity and electrochemical signals, combining context information such as clothes material, washing temperature and laundry detergent type, performing normalization, de-noising and temperature compensation pre-processing, and calculating the water quality of the washing water; inputting into a multi-scale convolution auto-encoder to realize decoupling separation of pollution features and background interference features; a gating fusion mechanism is utilized, key features are dynamically screened, a reusability discrimination vector is generated, a water quality score and a waterway control signal are output through a lightweight student model, intelligent switching of a water circulation path is realized, the water quality evaluation precision is improved, the method has interpretability and visual analysis capability on a discrimination process, and the method is suitable for popularization and application. The method is suitable for efficient utilization of water resources under diversified washing situations.

The application discloses a method for establishing a gynecological menstruation-regulating tablet fingerprint spectrum by using a high-performance liquid chromatography technology and application, by using the method, a good fingerprint spectrum is obtained, and the chromatographic peak is comprehensive, 12 fingerprint peaks are confirmed, wherein, corresponding attribution peaks of angelica sinensis, fried atractylodes macrocephala koidz, vinegar corydalis and chuanxiong are all in the gynecological menstruation-regulating tablet, and the angelica sinensis, fried atractylodes macrocephala koidz and vinegar corydalis all have exclusive peaks. By comparing with the reference substance, and combining with ultraviolet absorption spectrum characteristics, characteristic peaks of five chemical components, i.e., chlorogenic acid, ferulic acid, chuanxiongolide I, ligusticum lactone and butenyl phthalide are identified. The application further discloses a reference fingerprint spectrum of the gynecological menstruation-regulating tablet obtained by using the method, the gynecological menstruation-regulating tablet fingerprint spectrum detection method and the obtained reference fingerprint spectrum can objectively evaluate the overall quality of the gynecological menstruation-regulating tablet, and provide a research basis for quality control of the gynecological menstruation-regulating tablet.

The application discloses a sunscreen composition based on the ultraviolet absorption characteristics of yellow water of liquor, and a preparation method and application thereof. The sunscreen composition comprises 10-60 parts by weight of a yellow water extract of a strong-flavor liquor, 20-70 parts by weight of a yellow water extract of a Maotai-flavor liquor, and 0-40 parts by weight of a yellow water extract of a light-flavor liquor. It is found that the yellow water of different flavor liquors has characteristic ultraviolet absorption spectrum, wherein the yellow water of the strong-flavor liquor has strong absorption in the UVB band (290-320 nm), the yellow water of the Maotai-flavor liquor has strong absorption in the UVA band (340-380 nm), and the yellow water of the light-flavor liquor has relatively uniform absorption in a wide band. According to the spectral complementarity, the natural sunscreen composition is obtained. The sunscreen composition has an in-vitro SPF value of 15-35, a PA value of ++~++++, good light stability and low skin irritation.

The invention discloses a method for testing the ink transfer rate of an intaglio printing organic photoactive layer and application. The test method comprises the following steps: preparing active layer ink into a reference solution, and measuring a first absorption curve; printing the same amount of active layer ink into a film in an intaglio printing manner; collecting ink attached to the surface of the scraper, preparing the ink into a scraper ink aqueous solution, and measuring a second absorption curve; collecting ink in the film layer, preparing a film-forming ink aqueous solution, and measuring a third absorption curve; and calculating the transfer rate of the ink based on the corresponding characteristic peak in the absorption curve. According to the test method provided by the invention, the printed active layer film is dissolved in the solvent, sufficient dissolution can be ensured in cooperation with ultrasound, the problem of weighing method misalignment caused by the phenomenon that the thickness of the printed film is possibly uneven is avoided, errors can be reduced by testing the ultraviolet absorption spectrum of the solution, and the test precision and reproducibility are remarkably improved.

The utility model provides a dual-light-source deep ultraviolet miniature optical nitrate nitrogen turbidity sensor, which solves the problem that turbidity particles can interfere with an ultraviolet absorption spectrum of nitrate nitrogen and influence the detection accuracy in the prior art. A dual-light-source deep ultraviolet miniature optical nitrate nitrogen turbidity sensor comprises a detection cavity, and a light-emitting assembly and a receiving assembly are arranged on the two sides of the detection cavity; a deep ultraviolet light source emitter and a turbidity light source emitter are arranged in the light emitting assembly, a deep ultraviolet light source receiver and a turbidity light source receiver are arranged in the receiving assembly, the deep ultraviolet light source receiver is arranged opposite to the deep ultraviolet light source, and the turbidity light source receiver is arranged opposite to the turbidity light source. As the measurement result of the nitrate nitrogen is possibly interfered by the turbidity of the water body, the sensor can measure the turbidity of the water body firstly by integrating the turbidity light source and the receiver of the turbidity light source, and correspondingly compensate the measurement result of the nitrate nitrogen so as to reduce the interference of the turbidity on the measurement of the nitrate nitrogen.

The invention discloses a silver ion fluorescent probe based on pyrrolo-pyrrole aza-BODIPY as well as a preparation method and application of the silver ion fluorescent probe. The probe has a specific chemical structure, and alkynyl groups are modified at two ends of the probe. When the probe is combined with silver ions, the ultraviolet absorption spectrum of the probe has absorption peak widening and red shift within the range of 680-690nm, and meanwhile, the fluorescence emission of the probe under the excitation of 650nm is remarkably quenched, so that the specific dual-mode detection of the silver ions is realized. The probe shows high selectivity and high sensitivity on silver ions, the detection limit can reach the nanomole level, the anti-interference capability is high, and the probe can work in water or various organic solvents. The invention also provides a clear synthetic route of the fluorescent probe. The probe is suitable for rapid and sensitive detection of silver ions in environmental or biological samples.

The invention provides a strong-conjugation high-polarity ultraviolet absorbent as well as a preparation method and application thereof. According to the invention, a triazine derivative structure of a hyperconjugated system of (para-ester-triazine-BP-2-triazine-para-ester) is designed, the conjugated system is extended, charges in molecules are transferred, an ultraviolet absorption spectrum is widened, and an ultraviolet light absorber with a strong absorption effect on UVA and UVB is formed. The para-ester is introduced into the ultraviolet absorbent, and the vinyl sulfone group of the para-ester can be subjected to Michael addition reaction with amino (-NH2) in nylon to form a stable covalent bond, so that the ultraviolet absorbent is firmly combined on nylon fibers, and the washability is enhanced.

This invention discloses a method for identifying fresh / dried cocoon raw silk based on ultraviolet absorption spectroscopy. The method includes: pre-treating and extracting raw silk samples from fresh and dried cocoons to obtain a test solution; collecting the ultraviolet absorption spectra of the test solution and ultrapure water, and obtaining a corrected ultraviolet absorption spectrum after background signal correction; pre-processing the corrected ultraviolet absorption spectrum to obtain a pre-processed spectrum and its derivative spectrum; performing difference analysis to screen difference scoring indicators, and then establishing a discrimination model for fresh and dried cocoons; obtaining the pre-processed spectrum and its derivative spectrum of the raw silk sample to be identified, inputting them into the discrimination model for processing, and outputting the category to complete the identification of fresh and dried cocoon raw silk. This invention is convenient to operate, uses relatively common instruments, has high accuracy, and the accuracy can be further improved with increasing sample size, making it easy to promote and apply, and meeting the needs of identifying fresh / dried cocoon raw silk.

The invention belongs to the technical field of dangerous goods detection and analysis, and particularly relates to a preliminary screening method for judging heroin relevance. The method comprises the following steps: carrying out gradient elution on eight alkaloids in heroin by adopting ultra-high performance liquid chromatography, confirming peaks of the eight alkaloids by combining an ultraviolet absorption spectrum and retention time, and calculating the concentration of each alkaloid through a peak area external standard method of each alkaloid. The method comprises the following steps: performing specific ratio combination on eight alkaloid concentration values to form 14 specific alkaloid concentration ratio groups, and performing z fraction standardization on the ratio combinations to eliminate the dimensional difference between the alkaloid concentrations and the influence of heroin hydrolysis on the concentration content of each alkaloid in heroin. And finally, calculating the relevance among the real captured heroin samples by using a Pearson coefficient method. The method is accurate in discrimination result, low in cost and simple to operate, and can be used as a preliminary screening method for heroin relevance discrimination.

The present application relates to the technical field of analytical chemistry, in particular to a method for simultaneously and rapidly determining the content of two-component triazine ultraviolet absorbers in multiple solvents, comprising the following steps: S1. preparation of standard solution, S2. drawing of ultraviolet absorption spectrum, S3. selection of optimal wavelength, S4. drawing of standard curve, preparing standard solutions with different concentrations of two-component triazine ultraviolet absorbers, drawing a standard curve according to the first derivative value of the absorbance at the optimal wavelength selected above, S5. determination of sample content, taking a mixed triazine ultraviolet absorber sample for determination, and calculating the content according to the standard curve. The present application uses the first derivative spectrophotometry to simultaneously and rapidly determine the content of two-component triazine ultraviolet absorbers after mixing in multiple solvents, eliminates the interference of another component to be measured, has high accuracy, does not need complex pretreatment and separation process, is easy and fast to operate, and has low technical and equipment basic requirements.

The invention provides a method for rapidly detecting the content of total flavonoids in ginkgo biloba extract based on ultraviolet spectrum, which comprises the following steps: taking a ginkgo biloba extract sample, and treating by adopting an ultrasonic-assisted extraction method to prepare flavone extract liquor; scanning the flavone extracting solution by using an ultraviolet-visible spectrophotometer to obtain ultraviolet absorption spectrum data of the flavone extracting solution in a characteristic wavelength range; and inputting the obtained ultraviolet absorption spectrum data into a pre-generated general flavone content prediction model, and directly outputting to obtain the content value of general flavone in the ginkgo leaf extract. According to the method, interference of coexisting substances in a traditional ultraviolet method is effectively overcome through a chemometrics algorithm, detection is completed within a few minutes, and the accuracy of the method is equivalent to that of a high performance liquid chromatography. The method is easy and convenient to operate, low in cost, high in efficiency and particularly suitable for rapid quality control and raw material screening in the production process of the ginkgo leaf extract.

The application discloses a kind of long afterglow base ratio type aptamer sensor construction and carbendazim detection application, belong to spectral analysis technical field.The application is based on zinc germanate manganese-doped long afterglow material (ZGM) and the methylene blue with fluorescent characteristic constructs the ratio type aptamer sensor without complementary chain, using the overlapping characteristics of ultraviolet absorption spectrum and ZGM phosphorescence emission spectrum of methylene blue, through resonance energy transfer mechanism, realize the ZGM phosphorescence quenching and methylene blue fluorescence enhancement of probe, and then construct the long afterglow ratio type aptamer sensor without complementary chain.The characteristic emission peak of the long afterglow ratio type aptamer sensor is at the green phosphorescence emission of 542nm and the fluorescence emission of 700nm, the probe has the advantages of without in-situ excitation, without background interference and exogenous interference, high specificity, can sensitively, accurately and specifically detect CBZ residue in food sample.

The invention relates to the technical field of plasma physical and chemical diagnosis, in particular to an optical quantitative diagnosis system and method for space-time resolution of plasma active species. In order to solve the problems that full coverage of gas-phase species and liquid-phase species is difficult to realize at the same time and light path switching is complicated and time sequence collaboration is poor during multi-technology fusion diagnosis in the prior art, the following technical scheme is provided: the device comprises a light source system, a detection system, a time sequence control system and a plasma generation system. The device combines laser-induced fluorescence, a photochemical fluorescence probe and an ultraviolet absorption spectrum technology, has the advantages of integration, high sensitivity, spatial-temporal resolution and the like, realizes synchronous, quantitative and in-situ measurement of key active particles in a plasma gas phase and a plasma liquid phase, accurately analyzes the gas-liquid mass transfer and liquid phase permeation processes, and has a good application prospect. And a technical support is provided for realizing accurate control on the components and dosage of plasma active species and promoting the development of plasma biomedicine.

This application relates to a method for constructing an LCMS reading model and the application of the LCMS reading model. The construction method includes: acquiring LCMS training data, which includes training samples and corresponding training labels; wherein, the training samples include at least one of material structure features, mass spectrum features, ultraviolet absorption spectrum (UV) features, MS-UV correlation features, and instrument parameter features; the training labels include reading results corresponding to at least one business type; and iteratively training the initial model based on the LCMS training data to obtain a trained LCMS reading model, which is used to predict the target reading results corresponding to the business type. The solution provided in this application can efficiently and accurately output reading results for different business types, providing a reliable guarantee for the automated preparation process of products.

This invention relates to spectroscopic technology, specifically providing a method for oil analysis in water based on the fusion of ultraviolet absorption spectroscopy and three-dimensional fluorescence spectroscopy, comprising the following steps: A1. Obtaining oil samples of different types and concentrations, and obtaining the corresponding ultraviolet absorption spectra and three-dimensional fluorescence spectra; A2. Expanding the three-dimensional fluorescence data and ultraviolet absorption spectral data, corresponding to the excitation wavelength, and stitching the two together to obtain the fused spectrum, with absorbance as a separate column; A3. Decomposing the fused spectrum X and the dependent variable matrix Y; obtaining a set of T and U with the maximum covariance, thereby obtaining the model B=(T T T) ‑1 T T U; A4. Obtain a water sample, obtain the corresponding fusion spectrum according to A1 and A2, and use the model to obtain Y, thereby determining the type of oil in the water and the corresponding concentration. This invention has advantages such as accuracy.

The application relates to a COD online detection turbidity compensation method based on ultraviolet absorption spectrum, which comprises the following steps: preparing gradient COD standard solution, gradient turbidity standard solution and mixed solution, and measuring the absorption spectrum of various solutions; a COD concentration prediction model and a turbidity prediction model are established; the ultraviolet absorption spectrum of a mixed solution to be measured is collected, the characteristic wavelength absorbance is extracted and substituted into the turbidity prediction model to obtain a predicted turbidity value, a turbidity interference correction coefficient and an interference reference value are calculated, and the corrected COD true absorbance is generated; the corrected COD true absorbance is subjected to interval average processing, the comprehensive abnormal value is calculated, and the detection effectiveness is judged. The application has high reliability, can reduce the average relative error of COD detection through optimized modeling interval screening, multi-wavelength collaborative turbidity identification and scene compensation strategies, and cooperates with a closed-loop abnormal judgment mechanism, so that the detection result is still accurate and stable under a complex turbid water quality environment, and the actual water quality treatment can be effectively guided.

The invention discloses a method for determining the molar absorption coefficient of bisulfate ions in a solution. The method comprises the following steps: analyzing ultraviolet absorption spectrums of sulfuric acid, sodium hydrogen sulfite and sodium sulfate solutions by adopting an ultraviolet-visible spectrophotometric method, and calculating molar absorption coefficients of HSO4 <-> under different wavelengths by integrating absorbance values and ionization constants of HSO4 <-> in different solutions. The method for calculating the molar absorption coefficient of HSO4 <-> in the wavelength range of 189-200 nm is established by measuring the absorbance of a series of low-concentration solutions containing sulfuric acid, sodium hydrogen sulfate and sodium sulfate. Then, the total sulfur content in the binary mixture of sodium sulfate / sulfuric acid and sodium hydrogen sulfate / sulfuric acid is determined by utilizing the numerical values, and compared with an experimental result, the relative error is 3.82% + / -2.11%, so that the reliability of the determination method disclosed by the invention is proved.

The application discloses a method for establishing a Qingre Mingmu tea fingerprint spectrum by using a high performance liquid chromatography method and application. By using the method, 17 fingerprint peaks can be confirmed, and three components of fried cassia seed, chrysanthemum and sweetleaf chrysanthemum in the Qingre Mingmu tea have corresponding attribution peaks. By comparison with reference substances and in combination with ultraviolet absorption spectrum characteristics, characteristic peaks of six chemical components, i.e., chlorogenic acid, rebaudioside A, stevioside, citranaxanthin, emodin and chrysophanol, are identified, wherein the rebaudioside A and the stevioside are characteristic peaks of the sweetleaf chrysanthemum, and the two characteristic peaks can distinguish the chrysanthemum and the sweetleaf chrysanthemum. The fingerprint spectrum detection method disclosed by the application can obtain a fingerprint spectrum with good separation degree and comprehensive chromatographic peaks, can more objectively and comprehensively evaluate the overall quality of the Qingre Mingmu tea, and lays a foundation for quality control of the Qingre Mingmu tea.

The present application belongs to the technical field of dangerous goods detection and analysis, and particularly relates to a preliminary screening method for discriminating heroin correlation. The present application adopts ultra-high performance liquid chromatography to gradient elute 8 alkaloids in heroin, combines ultraviolet absorption spectrum and retention time to confirm 8 alkaloid peaks, and calculates the concentration of each alkaloid through the peak area external standard method of each alkaloid. The concentration values of the 8 alkaloids are combined into specific ratio combinations, 14 specific alkaloid concentration ratio groups are combined, and z-score standardization is performed on these ratio combinations to eliminate the dimensional differences between the concentrations of each alkaloid and the influence of heroin hydrolysis on the concentration content of each alkaloid in heroin. Finally, the Pearson coefficient method is used to calculate the correlation between the real seized samples of heroin. The discrimination result of the present application is accurate, the cost is low, the operation is simple, and the present application can be used as a preliminary screening method for discriminating heroin correlation.

The application discloses a method for establishing a Hongqu fingerprint spectrum by using a high performance liquid chromatography technology and application of the method in quality detection of Shiguogong medicinal liquor, the Hongqu fingerprint spectrum obtained by using the method has good separation of each chromatographic peak, a smooth baseline, a good peak type, and good precision, repeatability, specificity and stability, seven fingerprint peaks of the Hongqu fingerprint spectrum established by the application are confirmed, and by comparison with reference substances and in combination with ultraviolet absorption spectrum characteristics, characteristic peaks of main chemical components, namely, open-loop lovastatin and lovastatin in the Hongqu are identified, the method can be used for quality detection of the Hongqu, and provides a scientific basis for improving a production process and quality control of the Hongqu, and can also be applied to quality detection of Hongqu components in the Shiguogong medicinal liquor, and has important significance for guaranteeing the quality of the Hongqu in the Shiguogong medicinal liquor.

The resin composition of the present invention comprises a copolymer of structural units derived from ethylene and structural units derived from α-olefins having carbonyl groups, a brominated flame retardant, and antimony trioxide. In the resin composition, the copolymer is present in an amount of 20% by mass or more and 60% by mass or less. In the resin composition, the brominated flame retardant is present in an amount of 10% by mass or more and 30% by mass or less. In the resin composition, the antimony trioxide is present in an amount of 2% by mass or more and 15% by mass or less. The resin composition further comprises at least two compounds selected from the group consisting of a first compound, a second compound, and a third compound. In the resin composition, each of the at least two compounds selected from the group consisting of the first compound, the second compound, and the third compound is present in an amount of 0.01% by mass or more and 1.5% by mass or less. The highest absorption peak in the ultraviolet absorption spectrum of the first compound is located in the region of 320 nm or more and 360 nm or less. The highest absorption peak in the ultraviolet absorption spectrum of the second compound is located in the region of 275 nm or more and less than 320 nm. The highest absorption peak in the ultraviolet absorption spectrum of the third compound is located in the region of 240 nm or more and less than 275 nm.

The invention discloses a preparation method of a PVP (Polyvinyl Pyrrolidone) functionalized NH2-Ti-MOF dual-mode probe, PVP is used as a shape control agent, 2-aminoterephthalic acid is used as a ligand (NH2-BDC), tetrabutyl titanate (TBOT) is used as a metal source to synthesize the PVP functionalized NH2-Ti-MOF, the interaction between catechol and the PVP-NH2-Ti-MOF is utilized to synthesize the dual-mode probe of the PVP functionalized NH2-Ti-MOF, and the dual-mode probe of the PVP functionalized NH2-Ti-MOF is used to synthesize the dual-mode probe of the PVP functionalized NH2-Ti-MOF. A sensor for detecting CC in dual modes is constructed. When the CC is added into a PVP-NH2-Ti-MOF solution, in a fluorescence spectrum, the fluorescence intensity at 428 nm is reduced, and a new ultraviolet absorption peak appears at 282 nm in an ultraviolet absorption spectrum, so that fluorescence and colorimetric dual-mode detection of the CC is realized based on a static quenching effect, and the detection limits are respectively as low as 0.26 M and 0.077 M. Compared with a single-mode sensor, the dual-mode sensor is more accurate, quicker and more sensitive, and a new thought is provided for detection of CC in a water sample.

This invention discloses a rapid method for detecting the residual amount of polyacrylamide in manufactured sand, comprising: (1) mixing the manufactured sand sample to be tested with purified water, and extracting and filtering to obtain a filtrate containing polyacrylamide; (2) taking the filtrate prepared in step (1), detecting its ultraviolet absorption spectrum at 574 nm using the starch-cadmium iodide indicator method, and then obtaining the ultraviolet absorption spectrum data of polyacrylamide in the filtrate as A by comparing it with the control group; (3) substituting data A into the standard curve of polyacrylamide to obtain the concentration of polyacrylamide in the filtrate, and then converting it to obtain the residual amount of polyacrylamide in the manufactured sand. This invention discloses a method for detecting the residual amount of polyacrylamide in manufactured sand, which can rapidly and accurately measure the content of flocculant polyacrylamide in manufactured sand and reduce its impact on concrete performance.

The invention relates to application of tribonema in preparation of an ultraviolet absorbent, and a tribonema extract and a skin care composition with an anti-ultraviolet function. The invention finds that the tribonema extract has a very wide ultraviolet absorption spectrum, has a plurality of absorption peaks between 280-400 nm, and almost covers all UVA and UVB wavebands. On the basis, the preparation steps of the tribonema extract are optimized, and the tribonema extract capable of preventing cells cultured in vitro from being damaged by ultraviolet rays and the skin cream with the sunscreen function are obtained.

The invention relates to a method for simultaneously determining salicylic acid and acetylsalicylic acid. The method is based on ultraviolet-visible spectrophotometry, iron ions and salicylic acid containing phenolic hydroxyl groups form a complex for developing, the problem that ultraviolet absorption spectrums of salicylic acid and acetylsalicylic acid are overlapped is successfully solved, and a linear relation between concentration and absorbance of salicylic acid and acetylsalicylic acid in different absorption wavelengths is established. Therefore, simultaneous quantitative determination of the two is realized. The method has the advantages of high selection specificity, high accuracy, low analysis cost, rapidness, simplicity and convenience in operation and the like. The method can be used for quantitative determination and purity determination of acetylsalicylic acid in actual production and scientific research, and determination of acetylsalicylic acid hydrolysis rate and drug validity period, can also be used for evaluating results of acetylsalicylic acid preparation experiments, provides teaching reference for chemical experiment courses in colleges and universities, and has a wide application prospect.

The invention relates to the technical field of spectral analysis, and discloses a cross-instrument water quality detection method adaptive to ultraviolet-visible absorption spectrum characteristics, which comprises the following steps: determining a master instrument and a slave instrument for collecting ultraviolet absorption spectrum data; obtaining standard spectrums of all samples on the main instrument; performing difference analysis on the ultraviolet absorption spectrum data of the sample acquired from the instrument and a corresponding standard spectrum so as to find an optimal sliding window size and a reference wavelength point; establishing a unary linear correction equation based on the size of the optimal sliding window and the reference wavelength point, and solving the unary linear correction equation through absorbance data at the reference wavelength point so as to obtain a difference transfer model between the master instrument and the slave instrument; and correcting the first spectral data collected by the slave instrument by using the difference transfer model, and inputting the corrected spectral data into the ultraviolet spectrum analysis model established based on the master instrument, thereby realizing cross-instrument detection of the sample. The detection cost is reduced.

The invention discloses a single-molecule fluorescent nanoparticle carrier for protein delivery as well as a screening method and application of the single-molecule fluorescent nanoparticle carrier. The carrier is a compound formed by modifying dipeptide on the surfaces of monomolecular fluorescent nanoparticles (FUNs). The core of the screening method is as follows: firstly, constructing a display library containing nearly 400 different dipeptides on the surface of FUNs through a parallel synthesis technology; then, carrying out high-flux and label-free preliminary screening by calculating aggregation parameters according to the change of an ultraviolet absorption spectrum after the carrier is combined with the protein; and molecular docking software such as Autolock and the like can be combined to evaluate the binding force to carry out auxiliary screening. The invention further provides a method for verifying carrier delivery efficiency and treatment effect by using the cell line and the mouse model. The method is simple in screening process and high in universality, and the obtained vector can efficiently deliver multiple functional proteins such as ovalbumin and CRISPR-Cas9 and shows good application prospects in disease treatment and prevention.