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Skin care essence solution based on human mesenchymal stem cell secretion activity factors and preparation method of skin care essence solution

A technology for skin care essence and stem cells, which is applied in the field of skin care essence based on active factors secreted by human mesenchymal stem cells and its preparation, to achieve the effects of promoting skin metabolism, promoting damage repair and eliminating inflammation

Inactive Publication Date: 2017-09-22
TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is used in cosmetics to repair various skin problems of the face and the body, and there is no report

Method used

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  • Skin care essence solution based on human mesenchymal stem cell secretion activity factors and preparation method of skin care essence solution
  • Skin care essence solution based on human mesenchymal stem cell secretion activity factors and preparation method of skin care essence solution
  • Skin care essence solution based on human mesenchymal stem cell secretion activity factors and preparation method of skin care essence solution

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preparation example Construction

[0021] The preparation method of the skin care essence based on the active factors secreted by human mesenchymal stem cells of the present invention includes the following steps:

[0022] (1) Human mesenchymal stem cell culture: P2-P6 human mesenchymal stem cells are selected from the cell bank for recovery, the inoculation batches are recorded as the first batch, the second batch, and the third batch, and the inoculation density is the maximum fusion. The temperature is 50%-60%, 35%-45%, 20%-30%; the cell inoculation is completed, and the cells are cultured in a carbon dioxide incubator at a temperature of 37°C and a carbon dioxide volume ratio of 5%;

[0023] (2) When the fusion degree of the first batch of cells reaches 70%-80%, wash the cells twice with normal saline, and use compound electrolyte injection for starvation culture;

[0024] (3) After 12-24 hours of starvation culture, the first batch of cells has a fusion degree of 90%-95%, collect the cell culture supernatant, cen...

Embodiment 1

[0037] The preparation method of the skin care essence based on the active factors secreted by human mesenchymal stem cells of the present invention includes the following steps:

[0038] (1) Mesenchymal stem cell culture: P2 umbilical cord mesenchymal stem cells are selected from the cell bank for recovery, the inoculation batches are recorded as the first batch, the second batch, and the third batch, and the inoculation density is the maximum confluence. 60%, 40%, 25% of the cell inoculation; put the cells in a carbon dioxide incubator for cultivation at a temperature of 37°C, and a carbon dioxide volume ratio of 5%;

[0039] (2) After 24 hours, when the fusion degree of the first batch of cells reaches 75%, wash the cells twice with normal saline and use 40ml compound electrolyte injection (compound electrolyte glucose MG3 injection produced by China Otsuka Pharmaceutical Co., Ltd.) Starvation culture

[0040] (3) After 20 hours of starvation culture, the first batch of cells has...

Embodiment 2

[0049] The preparation method of the skin care essence based on the active factors secreted by human mesenchymal stem cells of the present invention includes the following steps:

[0050] (1) Mesenchymal stem cell culture: select P4 adipose-derived mesenchymal stem cells from the cell bank for resuscitation. The inoculation batches are recorded as the first batch, the second batch, and the third batch, and the inoculation density is the maximum confluence. 65%, 45%, 30% of the cell inoculation. After the cell inoculation is completed, place it in a carbon dioxide incubator at a temperature of 37°C and a carbon dioxide volume ratio of 5%;

[0051] (2) After 20 hours, when the confluency of the first batch of cells reaches 75%, wash the cells twice with normal saline, and use 40ml compound electrolyte injection for starvation culture;

[0052] (3) After 24 hours of starvation culture, the first batch of cells has a fusion degree of 90%, collect the cell culture supernatant, centrifuge ...

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Abstract

The invention discloses a skin care essence based on human mesenchymal stem cells secreting active factors and a preparation method thereof. Human mesenchymal stem cells secrete a variety of large amounts of cytokines during the culture process, and these cytokines can penetrate deep into the basal layer of the skin to promote skin care. Tissue differentiation, angiogenesis and growth of granulation tissue promote the structural reconstruction and regeneration of damaged skin tissue, promote the removal of aging cells and the regeneration of cells, so as to maintain and restore the youthful state of the skin. Compared with the traditional skin care essence, the essence based on the active factors secreted by mesenchymal stem cells can fundamentally solve the aging problem of the skin, and has no toxic side effects. In the present invention, the human mesenchymal stem cell secretion is extracted after repeated starvation culture, and contains compound electrolyte injection and other ingredients. It can be used to relieve and repair skin aging problems caused by various reasons.

Description

Technical field [0001] The invention relates to a skin care essence, in particular to a skin care essence based on the secretion of active factors by human mesenchymal stem cells and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (MSC) are a group of heterogeneous cell populations derived from the matrix, which can be obtained from most tissues of the human body. They have the ability of self-renewal, tissue repair, immune regulation, and can differentiate into the mesodermal lineage, such as adipocytes, bone cells, chondrocytes, etc., and can also differentiate into other germ layer lineage cells, such as epidermal cells / vascular endothelial cells Differentiation. [0003] The mesenchymal stem cell culture system contains a large number of active ingredients, such as: stem cell growth factor (SCF), fibroblast growth factor (FGF), endothelial cell growth factor (VEGF), epidermal cell growth factor (EGF), cell More than 80 active substances such...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/64A61Q19/00A61Q19/08
CPCA61K8/99A61K8/64A61Q19/00A61Q19/08
Inventor 张冰晶鲁振宇韩洪起秦臻熊亮高婧彧徐悦黄文敬闻婧
Owner TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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