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Body cream based on active factor extract secreted by human mesenchymal stem cells, and preparation method thereof

A technology of stem cells and active factors, applied in the field of stem cell extracts, to solve skin problems, reduce bacterial infection and inflammation, and promote regeneration and repair

Inactive Publication Date: 2017-09-08
TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on cosmetics for repairing various skin problems on the face and body

Method used

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  • Body cream based on active factor extract secreted by human mesenchymal stem cells, and preparation method thereof
  • Body cream based on active factor extract secreted by human mesenchymal stem cells, and preparation method thereof
  • Body cream based on active factor extract secreted by human mesenchymal stem cells, and preparation method thereof

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preparation example Construction

[0022] The preparation method of the body cream based on the active factor extract secreted by human mesenchymal stem cells of the present invention comprises the following steps:

[0023] (1) Human mesenchymal stem cell culture: P2-P6 human mesenchymal stem cells were selected from the cell bank for resuscitation, the inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was the maximum fusion 50%-60%, 35%-45%, 20%-30% of the degree; the complete medium used for culturing cells is phenol red-free DMEM / DF12 and the volume ratio concentration is 10% fetal bovine serum; the cell inoculation is completed and placed in Cultivate in a carbon dioxide incubator at a temperature of 37°C and a concentration of 5% by volume of carbon dioxide;

[0024] (2) When the fusion degree of the first batch of cells reaches 70%-80%, wash the cells twice with normal saline, and perform starvation culture with compound electrolyte in...

Embodiment 1

[0038] (1) Culture of human umbilical cord mesenchymal stem cells: P4 human umbilical cord mesenchymal stem cells were recovered from the cell bank. The inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was 60% of the maximum fusion degree (the number of inoculated cells per bottle: 9*10 6 , ten bottles of cells inoculated), 40% (the number of cells inoculated in each bottle: 6*10 6 , ten bottles of inoculated cells), 20% (number of inoculated cells: 3*10 6, inoculated cells in ten flasks), the culture system was 40ml. The complete medium used for culturing the cells is phenol red-free DMEM / DF12 and 10% fetal calf serum by volume, and the bottle for inoculating the cells is a T-175 culture flask. After the cell inoculation is completed, place it in a carbon dioxide incubator for cultivation at a temperature of 37°C and a volume ratio concentration of carbon dioxide of 5%;

[0039] (2) Cultivate for 30 ho...

Embodiment 2

[0050] (1) Culture of human adipose-derived mesenchymal stem cells: P3 human adipose-derived mesenchymal stem cells were recovered from the cell bank. The inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was 60% of the maximum fusion degree (the number of inoculated cells per bottle: 9*10 6 , ten bottles of cells inoculated), 40% (the number of cells inoculated in each bottle: 6*10 6 , ten bottles of inoculated cells), 20% (number of inoculated cells: 3*10 6 , inoculated cells in ten flasks), the culture system was 40ml. The complete medium used for culturing the cells is phenol red-free DMEM / DF12 and 10% fetal calf serum by volume, and the bottle for inoculating the cells is a T-175 culture flask. After the cell inoculation is completed, place it in a carbon dioxide incubator for cultivation at a temperature of 37°C and a volume ratio concentration of carbon dioxide of 5%;

[0051] (2) Cultivate for 24...

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Abstract

The invention discloses a body cream based on active factor extracts secreted by human mesenchymal stem cells and a preparation method thereof. Human mesenchymal stem cells secrete various and large amounts of cytokines during the culture process, and these cytokines can penetrate deep into the basal layer of the skin Promote skin tissue differentiation, angiogenesis and growth of granulation tissue, promote structural reconstruction and regeneration of damaged skin tissue, promote removal of aging cells and regeneration of cells, thereby maintaining and restoring youthful skin and reducing pigmentation. Compared with traditional body creams, body creams based on active factors secreted by mesenchymal stem cells can fundamentally solve the aging problem of the whole body skin without toxic side effects. In the present invention, the human mesenchymal stem cell secretion is extracted after repeated starvation culture, and contains compound electrolyte injection and other ingredients. It can be used for alleviating and repairing the skin aging problems of the whole body caused by various reasons.

Description

technical field [0001] The invention relates to stem cell extracts, in particular to a body cream based on active factor extracts secreted by human mesenchymal stem cells and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a group of heterogeneous cell populations derived from stroma, which can be obtained from most tissues of the human body. They have the ability of self-renewal, tissue repair, and immune regulation, and can differentiate to the mesoderm lineage, such as adipocytes, bone cells, chondrocytes, etc., and can also differentiate to other germ layer lineage cells, such as epidermal cells, vascular endothelium Cell Differentiation. [0003] The mesenchymal stem cell culture system contains a variety of active ingredients, such as: stem cell growth factor (SCF), fibroblast growth factor (FGF), endothelial cell growth factor (VEGF), epidermal growth factor (EGF), cell Colony-stimulating factor, interleukin (IL), collag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/9789A61Q19/00
CPCA61K8/99A61K8/97A61Q19/00
Inventor 熊亮张冰晶鲁振宇韩洪起秦臻高婧彧徐悦黄文敬
Owner TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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