Green moss charcoal-microorganism complex for improving saline-alkali soil and improving saline-alkali tolerance of plants as well as preparation method and application of green moss charcoal-microorganism complex

Abstract

The invention belongs to the technical field of soil improvement, and particularly relates to a green moss charcoal-microorganism complex for improving saline-alkali soil and improving saline-alkali tolerance of plants as well as a preparation method and application of the green moss charcoal-microorganism complex. The nitrogen-fixing bacteria have a nitrogen-fixing effect, the preristeria megatherium has a potassium-dissolving effect, the nitrogen-fixing bacteria and the preristeria megatherium are combined with the green moss-derived biochar, and the green moss biochar is small in aperture, can effectively adsorb metal cations in saline-alkali soil, has strong affinity to the nitrogen-fixing bacteria and the preristeria megatherium, and can effectively adsorb the metal cations in saline-alkali soil. The nitrogen-fixing bacteria and the P.megatherium can be effectively adsorbed, so that the colonization of the nitrogen-fixing bacteria and the P.megatherium on the surface of a plant root system and in rhizosphere soil is enhanced; meanwhile, the nitrogen-fixing bacteria and the preristeria megatherium attached to the biochar can relieve saline-alkali stress of the rhizosphere microenvironment, the resistance of crops to the saline-alkali environment is improved, the growth of the crops is promoted, and a new thought is provided for stress resistance and yield increase of the crops in the saline-alkali environment.

Description

Technical Field

[0001] The invention belongs to the technical field of soil improvement, and in particular relates to a moss biochar-microorganism complex for improving saline-alkali land and enhancing the salt-alkali tolerance of plants, and a preparation method and application thereof. Background Art

[0002] Soil salinization is a major problem facing the world's soil, causing a significant decline in the global area of ​​arable land, thus posing a huge threat to agricultural production. How to improve saline-alkali land, enhance the ability of plants to resist salt and alkali, and achieve efficient agricultural production on salinized land is a major challenge facing the world.

[0003] The classification index of saline-alkali land is as follows: slightly saline soil, soil salt content 0.1-0.5%; moderately saline soil, soil salt content 0.2-0.4%; severely saline soil, soil salt content 0.4-0.6%. Another index to measure saline-alkali land is soil acidity, i.e. pH value, which is generally around 7.5 for neutral, below 7.0 for acid, and above 7.5 for slightly alkaline. The coexistence of salt and alkali in saline-alkali land soil improvement determines its complexity. Although saline-alkali land improvement fertilizers are being introduced all over the country, the results are minimal. Summary of the invention

[0004] The purpose of the present invention is to provide a moss biochar-microorganism complex for improving saline-alkali land and improving the salt-alkali tolerance of plants, as well as a preparation method and application thereof, so as to effectively improve saline-alkali land and improve the salt-alkali tolerance of plants.

[0005] In order to achieve the above object, the present invention provides a moss biochar-microorganism consortium, wherein the moss biochar-microorganism consortium comprises moss biochar, nitrogen-fixing bacteria (Azotobacter sp.) and Priestia megaterium.

[0006] Preferably, the nitrogen-fixing bacteria is nitrogen-fixing bacteria BNCC335805; and the Priesteria megaterium is Priesteria megaterium NK851.

[0007] Preferably, the method for preparing the moss biochar comprises: pyrolyzing the dried moss at 500° C. for 1 to 12 hours.

[0008] The present invention also provides a method for preparing the green moss biochar-microorganism complex described in the above technical solution, comprising the following steps:

[0009] The nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the green moss biochar are mixed to obtain the green moss biochar-microorganism complex.

[0010] Preferably, the usage ratio of the nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the green moss biochar is (200-300) mL: (200-300) mL: (50-150) g;

[0011] The OD of the nitrogen-fixing bacteria culture solution 600 0.5~5;

[0012] The OD of the Priesteria megaterium culture solution 600 It is 0.5~5.

[0013] Preferably, the method for preparing the nitrogen-fixing bacteria culture solution comprises: inoculating the nitrogen-fixing bacteria into a Mart culture medium, and culturing at 30° C. and 160 rpm for 24 to 48 hours to obtain the nitrogen-fixing bacteria culture solution;

[0014] The method for preparing the nitrogen-fixing bacteria culture solution comprises: inoculating Priesteria gigantea into a Mart culture medium, and culturing for 24 to 48 hours at 30° C. and 160 rpm to obtain a Priesteria gigantea culture solution;

[0015] The Mart culture medium includes 10 g / L glucose, 5 g / L peptone, 1 g / L potassium dihydrogen phosphate, 0.25 g / L magnesium sulfate, 0.01 g / L ferrous sulfate and 5 g / L sodium chloride.

[0016] The present invention also provides a composition, comprising a substrate and a moss biochar-microorganism complex; the moss biochar-microorganism complex is the moss biochar-microorganism complex described in the above technical solution.

[0017] Preferably, the substrate comprises one or more of peat soil, bone meal, nutrient soil and bran;

[0018] Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the peat soil is 100g:5kg.

[0019] The present invention also provides the use of the moss biochar-microorganism complex or composition described in the above technical solution in improving saline-alkali land and / or improving the salt-alkali tolerance of plants.

[0020] The present invention also provides a method for improving saline-alkali land and / or improving the salt-alkali tolerance of plants, comprising one or two of the following:

[0021] (1) mixing the green moss biochar-microorganism complex described in the above technical solution with a matrix to obtain a moss biochar-growth-promoting microorganism complex-matrix;

[0022] applying the moss biochar-growth-promoting microorganism complex-matrix to saline-alkali soil;

[0023] Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the substrate is 100 g:5 kg;

[0024] The application amount of the moss biochar-growth-promoting microorganism complex-substrate is 100 kg / mu;

[0025] The substrate includes one or more of peat soil, bone meal, nutrient soil and bran;

[0026] (2) Applying the composition described in the above technical solution to saline-alkali soil; the application amount of the composition is 100 kg / mu.

[0027] Beneficial effects:

[0028] The present invention provides a green moss biochar-microorganism consortium, which includes green moss biochar, nitrogen-fixing bacteria and Priesteria gigantea. The nitrogen-fixing bacteria has a nitrogen-fixing effect, and Priesteria gigantea has a potassium-releasing effect. The two strains are combined with the biochar derived from green moss. The green moss biochar has a small pore size and can effectively adsorb metal cations in saline-alkali soils. It has a strong affinity for nitrogen-fixing bacteria and Priesteria gigantea, and can effectively adsorb nitrogen-fixing bacteria and Priesteria gigantea, thereby enhancing the colonization of nitrogen-fixing bacteria and Priesteria gigantea on the surface of plant roots and in rhizosphere soil; at the same time, the nitrogen-fixing bacteria and Priesteria gigantea attached to the biochar can alleviate the salt-alkali stress of the rhizosphere microenvironment, improve the resistance of crops to saline-alkali environments, and promote crop growth. The green moss biochar-microorganism consortium provided by the present invention provides a new idea for crop stress resistance and yield increase in saline-alkali environments. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0030] Figure 1 is the adsorption rate of different biochars on different microorganisms; groups without the same letters indicate significant differences among the groups (P<0.05);

[0031] Figure 2 Soil pH values ​​of wheat in different treatment groups after 90 days of growth; * indicates that the moss biochar-microbial consortium-peat soil treatment group is significantly different from other groups (P<0.05);

[0032] Figure 3 The total salt content of the soil after 90 days of wheat growth in different treatment groups; * indicates that the moss biochar-microbial consortium-peat soil treatment group is significantly different from other groups (P<0.05);

[0033] Figure 4 The actual pictures of wheat in different treatment groups after 90 days of growth;

[0034] Figure 5 The plant height of wheat in different treatment groups after 90 days of growth; * indicates that the moss biochar-microbial consortium-peat soil treatment group is significantly different from other groups (P<0.05);

[0035] Figure 6 is the wheat yield of different treatment groups; where * indicates that the green moss biochar-microbial consortium-peat soil treatment group is significantly different from other groups (P<0.05);

[0036] Figure 7 The plant height of barley in different treatment groups after 90 days of growth; * indicates that the moss biochar-microbial consortium-peat soil treatment group is significantly different from other groups (P<0.05);

[0037] Figure 8 Barley yields in different treatment groups; * indicates that the moss biochar-microbial consortium-peat soil treatment group was significantly different from other groups (P<0.05). DETAILED DESCRIPTION

[0038] The invention provides a moss biochar-microorganism complex, which comprises moss biochar, nitrogen-fixing bacteria and Priesteria gigantea.

[0039] In the present invention, the preparation method of the moss biochar preferably includes: pyrolyzing the dried moss at 500°C for 1 to 12 hours; the pyrolysis time is preferably 2 to 10 hours, and more preferably 5 to 8 hours. The moss biochar provided by the present invention has a significant pore structure, a small pore size, a strong affinity for plant growth-promoting microorganisms, and can effectively adsorb plant growth-promoting microorganisms, thereby enhancing the colonization of plant growth-promoting microorganisms on the surface of plant roots and in the rhizosphere soil. The results of the embodiment show that compared with straw biochar, moss biochar has a stronger adsorption capacity for microorganisms.

[0040] In the present invention, the nitrogen-fixing bacteria is preferably nitrogen-fixing bacteria BNCC335805, purchased from Beina Biotechnology; the Priestia megaterium is preferably Priestia megaterium NK851, which is disclosed in Wu, X., Zhao, Z., Zhao, Z., Zhang, Y., Li, M., & Yu, Q. (2023). Analysis of the potassium-solubilizing Priestiamegaterium strain NK851 and its potassium feldspar-binding proteins. International Journal of Molecular Sciences, 24 (18), 14226. The nitrogen-fixing bacteria and Priestia megaterium of the present invention can be attached to the green moss biochar, alleviate the saline-alkali stress of the rhizosphere microenvironment, improve the resistance of crops to saline-alkali environment, and promote crop growth.

[0041] The present invention also provides a method for preparing the green moss biochar-microorganism complex described in the above technical solution, comprising the following steps:

[0042] The nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the green moss biochar are mixed to obtain the green moss biochar-microorganism complex.

[0043] The present invention preferably prepares a nitrogen-fixing bacteria culture solution. In the present invention, the OD of the nitrogen-fixing bacteria culture solution is 600 Preferably, it is 0.5 to 5, more preferably 1 to 4, and more preferably 2. The method for preparing the nitrogen-fixing bacteria culture solution of the present invention preferably comprises: inoculating the nitrogen-fixing bacteria into the Mart culture medium, culturing for 24 to 48 hours at 30°C and 160rpm to obtain the nitrogen-fixing bacteria culture solution. The Mart culture medium of the present invention preferably comprises 10 g / L glucose, 5 g / L peptone, 1 g / L potassium dihydrogen phosphate, 0.25 g / L magnesium sulfate, 0.01 g / L ferrous sulfate and 5 g / L sodium chloride; the culturing time is preferably 30 to 45 hours, more preferably 36 hours.

[0044] The present invention preferably prepares a culture solution of Priesteria gigantea. In the present invention, the OD of the culture solution of Priesteria gigantea is 600Preferably, it is 0.5 to 5, more preferably 1 to 4, and more preferably 2. The method for preparing the nitrogen-fixing bacteria culture solution of the present invention preferably comprises: inoculating Priesteria gigantea into a Mart culture medium, culturing for 24 to 48 hours at 30°C and 160rpm to obtain a Priesteria gigantea culture solution; the Mart culture medium preferably comprises 10 g / L glucose, 5 g / L peptone, 1 g / L potassium dihydrogen phosphate, 0.25 g / L magnesium sulfate, 0.01 g / L ferrous sulfate, and 5 g / L sodium chloride; the culturing time is preferably 30 to 45 hours, more preferably 36 hours.

[0045] After obtaining the nitrogen-fixing bacteria culture solution and the Priesteria gigantea culture solution, the present invention preferably mixes the nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the green moss biochar to obtain the green moss biochar-microorganism association. In the present invention, the mixing temperature is preferably 20 to 30° C., more preferably 25° C.; the mixing time is preferably 10 to 60 minutes, more preferably 20 to 50 minutes, and more preferably 30 minutes.

[0046] In the present invention, the usage ratio of the nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the moss biochar is preferably (200-300) mL: (200-300) mL: (50-150) g, and more preferably 250 mL: 250 mL: 100 g.

[0047] The present invention also provides a composition, including a matrix and a moss biochar-microorganism complex; the moss biochar-microorganism complex is the moss biochar-microorganism complex described in the above technical solution. In the present invention, the matrix preferably includes one or more of peat soil, bone meal, nutrient soil and bran, and is further preferably peat soil. Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar to the matrix in the moss biochar-microorganism complex of the present invention is preferably 100g:5kg. The present invention combines the matrix and the moss biochar-microorganism complex, and the matrix can adsorb and protect the moss biochar-microorganism complex, maintain the activity of the bacteria, and facilitate subsequent uniform application.

[0048] The moss biochar-microorganism complex or composition provided by the present invention can enhance plant stress resistance in a saline-alkali environment and effectively increase crop yield.

[0049] In view of the effects of the moss biochar-microorganism complex and the composition provided by the present invention, the use of the moss biochar-microorganism complex or the composition of the present invention in improving saline-alkali land and / or improving the salt-alkali tolerance of plants also falls within the protection scope of the present invention.

[0050] In the present invention, the improving of plant salt-alkali tolerance preferably includes improving plant height and / or yield of saline-alkali land plants; the improving of saline-alkali land preferably includes improving the pH and / or total salt content of saline-alkali land; the pH value of saline-alkali land is preferably 7.5-9.0, more preferably 8.0-9.0; the total salinity of saline-alkali land is preferably 0.1%-0.3%, more preferably 0.2%-0.3%. The plants of the present invention preferably include wheat and / or barley.

[0051] The present invention also provides a method for improving saline-alkali land and / or improving the salt-alkali tolerance of plants, comprising one or two of the following:

[0052] (1) mixing the green moss biochar-microorganism complex described in the above technical solution with a matrix to obtain a moss biochar-growth-promoting microorganism complex-matrix;

[0053] applying the moss biochar-growth-promoting microorganism complex-matrix to saline-alkali soil;

[0054] Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the substrate is 100 g:5 kg;

[0055] The application amount of the moss biochar-growth-promoting microorganism complex-substrate is 100 kg / mu;

[0056] The substrate includes one or more of peat soil, bone meal, nutrient soil and bran;

[0057] (2) Applying the composition described in the above technical solution to saline-alkali soil; the application amount of the composition is 100 kg / mu.

[0058] In the present invention, the pH value of the saline-alkali soil is preferably 7.5-9.0, more preferably 8.0-9.0; the total salinity of the saline-alkali soil is preferably 0.1%-0.3%, more preferably 0.2%-0.3%. The present invention preferably applies nitrogen, phosphorus and potassium compound fertilizer while applying the moss biochar-growth-promoting microorganism association-matrix or composition; the total nutrient content of the nitrogen, phosphorus and potassium compound fertilizer is preferably ≥57%, and the mass ratio of N, P2O5 and K2O is preferably 19:19:19; the application amount of the nitrogen, phosphorus and potassium compound fertilizer is preferably 10-20 kg / mu, more preferably 15 kg / mu.

[0059] To further illustrate the present invention, the following detailed description is given of a moss biochar-microorganism complex for improving saline-alkali land and improving the salt-alkali tolerance of plants, as well as its preparation method and application in conjunction with the accompanying drawings and examples. However, they should not be construed as limiting the scope of protection of the present invention.

[0060] Example 1

[0061] Preparation of green moss biochar

[0062] Moss plants were collected from the wild, washed with running water to remove dust and impurities on the surface of the moss, and dried in an oven at 60°C to remove moisture. 10g of dried moss was weighed and placed in a reactor, which was then placed in a muffle furnace, heated at 500°C for 2h, and cooled at room temperature to obtain moss biochar. Scanning electron microscopy results showed that the moss biochar had a clear porous structure.

[0063] Comparative Example 1

[0064] Preparation of straw biochar

[0065] The straw was collected, washed with running water to remove dust and impurities on the surface of the straw, and dried in an oven at 60°C to remove moisture. 10 g of the dried straw was weighed and placed in a reactor, which was then placed in a muffle furnace, heated at 500°C for 2 h, and cooled at room temperature to obtain straw biochar.

[0066] Test Example 1

[0067] Adsorption performance of biochar on microorganisms

[0068] (1) Different microorganisms were inoculated into Mart liquid medium (glucose 10 g / L, peptone 5 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate 0.25 g / L, ferrous sulfate 0.01 g / L, sodium chloride 5 g / L, pH = 8.5), cultured at 30°C, 160 rpm for 24-48 h, centrifuged at 12000 rpm for 2 min, and the precipitate was collected; then the precipitate was suspended in phosphate buffered saline (PBS) at pH = 7.2, and the OD was finally measured. 600 =0.1, and obtain bacterial suspension;

[0069] The microorganisms are Agrobacterium tumefaciens LBA4404 (referred to as LBA4404), Pseudomonas putida KT2440 (referred to as KT2440), nitrogen-fixing bacteria BNCC335805 (referred to as BNCC335805) and Priesteria gigantea NK851 (referred to as NK851);

[0070] Among them, Agrobacterium tumefaciens LBA4404 was purchased from Takara;

[0071] Pseudomonas putida KT2440 was purchased from ATCC;

[0072] The nitrogen-fixing bacteria BNCC335805 were purchased from Beina Biotechnology;

[0073] Potassium-solubilizing Priestia megaterium strain NK851 is disclosed in Wu, X., Zhao, Z., Zhao, Z., Zhang, Y., Li, M., & Yu, Q. (2023). Analysis of the potassium-solubilizing Priestia megaterium strain NK851 and its potassium feldspar-binding proteins. International Journal of Molecular Sciences, 24(18), 14226.

[0074] (2) The moss biochar of Example 1 and the straw biochar of Comparative Example 1 were placed in the bacterial suspension obtained in step (1) at a concentration of 100 mg / L. After gently shaking at 25°C for 1 hour, the biochar was separated from the mixture. The microbial cells in the remaining bacterial suspension, i.e., the microbial cells not adsorbed by the biochar, were counted on a Mart culture medium plate by the colony forming unit (CFU) counting method, and the adsorption rate was calculated. The results are shown in FIG. Figure 1 shown.

[0075] according to Figure 1 It can be seen that the adsorption capacity of moss biochar for microorganisms is significantly higher than that of straw biochar. Compared with straw biochar, moss biochar can adsorb more than 90% of BNCC335805 and NK851. In addition, compared with Agrobacterium tumefaciens LBA4404 and Pseudomonas putida KT2440, moss biochar can adsorb higher amounts of BNCC335805 and NK851, indicating that moss biochar has a stronger adsorption capacity for nitrogen-fixing bacteria BNCC335805 and Priesteria gigantea NK851.

[0076] Example 2

[0077] Preparation of green moss biochar-symbiotic microbial consortium-peat soil matrix

[0078] (1) The nitrogen-fixing bacteria BNCC335805 was inoculated into 250 mL of Mart liquid culture medium and cultured at 30°C and 160 rpm for 24 h to obtain a nitrogen-fixing bacteria culture solution;

[0079] (2) inoculating Priesteria gigantea NK851 into 250 mL of Mart liquid culture medium, culturing at 30° C. and 160 rpm for 24 h to obtain Priesteria gigantea culture solution;

[0080] (3) mixing the nitrogen-fixing bacteria culture solution of step (1), the potassium-solubilizing Priesteria gigantea culture solution of step (2), and 100 g of the green moss biochar obtained in Example 1, and gently shaking at 25° C. for 30 min to obtain a green moss biochar-symbiotic microorganism complex;

[0081] (4) The moss biochar-symbiotic microorganism complex was mixed with 5 kg of peat soil matrix to obtain the moss biochar-symbiotic microorganism complex-peat soil matrix.

[0082] Comparative Example 2

[0083] Preparation of symbiotic microbial consortium-peat soil substrate

[0084] (1) The nitrogen-fixing bacteria BNCC335805 was inoculated into 250 mL of Mart liquid culture medium and cultured at 30°C and 160 rpm for 24 h to obtain a nitrogen-fixing bacteria culture solution;

[0085] (2) inoculating Priesteria gigantea NK851 into 250 mL of Mart liquid culture medium, culturing at 30° C. and 160 rpm for 24 h to obtain Priesteria gigantea culture solution;

[0086] (3) mixing the nitrogen-fixing bacteria from step (1) and the culture solution of Priesteria gigantea from step (2) to obtain a symbiotic microbial consortium;

[0087] (4) The symbiotic microbial consortium was mixed with 5 kg of peat soil matrix to obtain a symbiotic microbial consortium-peat soil matrix.

[0088] Comparative Example 3

[0089] Preparation of green moss biochar-peat soil matrix

[0090] The moss biochar obtained in Example 1 was mixed with a peat soil matrix in a mass ratio of 100 g:5 kg to obtain a moss biochar-peat soil matrix.

[0091] Test Example 2

[0092] Verification of wheat growth-promoting effect in saline-alkali land

[0093] (1) The experimental site was a saline-alkali land (pH 7.5-9.0, total salinity 0.2%-0.3%) in Binhai New Area, Tianjin, and wheat was planted. Specifically, the experimental site was randomly divided into 4 treatment areas and the following treatments were carried out:

[0094] The first treatment area (control group): Stanley Sanan compound fertilizer (N-P2O5-K2O: 19-19-19, total nutrients ≥ 57%) was applied to the soil of the test site at 15 kg / mu, peat soil substrate at 100 kg / mu, and wheat was planted in rows;

[0095] The second treatment area (symbiotic microbial consortium-peat soil matrix group): Stanley Sanan compound fertilizer (N-P2O5-K2O: 19-19-19, total nutrients ≥ 57%) was applied to the soil of the test site at 15 kg / mu, and the symbiotic microbial consortium-peat soil matrix of comparative example 2 was 100 kg / mu (based on the weight of the peat soil matrix), and wheat was planted in rows;

[0096] The third treatment area (green moss biochar-peat soil matrix group): Stanley Sanan compound fertilizer (N-P2O5-K2O: 19-19-19, total nutrients ≥ 57%) was applied to the soil of the test site at 15 kg / mu, and the green moss biochar-peat soil matrix of comparative example 3 was 100 kg / mu (based on the weight of the peat soil matrix), and wheat was planted in rows;

[0097] The fourth treatment area (moss biochar-symbiotic microbial association-peat soil matrix group): 15 kg / mu of Stanley Sanan compound fertilizer (N-P2O5-K2O: 19-19-19, total nutrients ≥ 57%) was applied to the soil of the test site, and 100 kg / mu of moss biochar-symbiotic microbial association-peat soil matrix of Example 2 (measured by the weight of peat soil matrix) was applied, and wheat was planted in rows.

[0098] (2) After 90 days of wheat planting, soil indicators, wheat growth indicators and yield were measured in each treatment group. The results are as follows: Figures 2 to 6 And as shown in Table 1.

[0099] Table 1 Plant height and yield data of wheat in each treatment group

[0100]

[0101]

[0102] According to the soil test results ( Figure 2 and Figure 3 ) It can be seen that compared with the control group, the symbiotic microbial consortium-peat soil matrix treatment group and the moss biochar-peat soil matrix treatment group, the soil pH of the moss biochar-symbiotic microbial consortium-peat soil matrix treatment group decreased significantly to only 7.85 ( Figure 2 ), the total salt content also decreased significantly, reaching 0.18% ( Figure 3 ). Therefore, the moss biochar-symbiotic microbial consortium-peat soil matrix can significantly reduce soil salinity and alkalinity, thereby improving soil quality.

[0103] According to the wheat growth index and yield test results ( Figures 4 to 6 and Table 1), it can be seen that the wheat growth in the moss biochar-symbiotic microbial consortium-peat soil matrix treatment group was significantly stronger than that in the other three groups ( Figure 4), the plant height was significantly higher than the other three groups, reaching more than 51cm ( Figure 5 and Table 1). Compared with the control group, the wheat yield of the moss biochar-symbiotic microbial consortium-peat soil matrix treatment group increased from 190 catties / mu to 483 catties / mu ( Figure 6 and Table 1). It can be seen that the green moss biochar-symbiotic microorganism association can significantly promote the growth of wheat in saline-alkali land and increase wheat yield.

[0104] Test Example 3

[0105] Verification of the growth-promoting effect on barley in saline-alkali soil

[0106] (1) The saline-alkali land (pH value of 8.0-9.0, total salinity of 0.2%-0.3%) in Binhai New Area of ​​Tianjin was used as the test site. Barley was planted according to the steps of Test Example 2. After 90 days of planting, the growth index and yield of barley were measured. The results are as follows: Figures 7-8 And as shown in Table 2.

[0107] Table 2 Barley plant height and yield data of each treatment group

[0108] Treatment Group Plant height(cm) Yield (catties / mu) Comparison 43±1.3 170±15 Symbiotic Microbial Consortium-Peat Soil Matrix 51±2.8 286±26 Green moss biochar-peat soil substrate 50±2.0 248±15 Green moss biochar-symbiotic microbial consortium-peat soil matrix 57±3.2 382±28

[0109] according to Figure 7 , Figure 8 As can be seen from Table 2, 90 days after barley planting, the growth of barley in the moss biochar-symbiotic microbial consortium-peat soil matrix treatment group was significantly stronger than that in the other three groups, and the plant height was significantly higher than that in the other three groups ( Figure 7 and Table 2), and barley yield was also significantly higher ( Figure 8 and Table 2). Therefore, the green moss biochar-symbiotic microorganism association can significantly promote the growth of barley in saline-alkali soil and increase its biomass.

[0110] It can be seen from the above content that the moss biochar-microorganism complex provided by the present invention can improve the pH and total salt content of saline-alkali land, improve the salt-alkali tolerance of plants, significantly promote the growth of plants in saline-alkali land, and increase their biomass.

[0111] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A moss biochar-microorganism complex, characterized in that: The moss biochar-microorganism complex comprises moss biochar, nitrogen-fixing bacteria (Azotobacter sp.) and Priestia megaterium.

2. The moss biochar-microorganism complex according to claim 1, characterized in that: The nitrogen-fixing bacteria is nitrogen-fixing bacteria BNCC335805; the Priesteria megaterium is Priesteria megaterium NK851.

3. The moss biochar-microorganism complex according to claim 1, characterized in that: The preparation method of the green moss biochar comprises: pyrolyzing the dried green moss at 500° C. for 1 to 12 hours.

4. The method for preparing the moss biochar-microorganism complex according to any one of claims 1 to 3, characterized in that: The steps include: The nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the green moss biochar are mixed to obtain the green moss biochar-microorganism complex.

5. The preparation method according to claim 4, characterized in that: The dosage ratio of the nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the green moss biochar is (200-300) mL: (200-300) mL: (50-150) g; The OD of the nitrogen-fixing bacteria culture solution 600 0.5~5; The OD of the Priesteria megaterium culture solution 600 It is 0.5~5.

6. The preparation method according to claim 4 or 5, characterized in that: The method for preparing the nitrogen-fixing bacteria culture solution comprises: inoculating the nitrogen-fixing bacteria into a Mart culture medium, and culturing for 24 to 48 hours at 30° C. and 160 rpm to obtain the nitrogen-fixing bacteria culture solution; The method for preparing the nitrogen-fixing bacteria culture solution comprises: inoculating Priesteria gigantea into a Mart culture medium, and culturing for 24 to 48 hours at 30° C. and 160 rpm to obtain a Priesteria gigantea culture solution; The Mart culture medium includes 10 g / L glucose, 5 g / L peptone, 1 g / L potassium dihydrogen phosphate, 0.25 g / L magnesium sulfate, 0.01 g / L ferrous sulfate and 5 g / L sodium chloride.

7. A composition, characterized in that It comprises a matrix and a moss biochar-microorganism complex; the moss biochar-microorganism complex is the moss biochar-microorganism complex described in any one of claims 1 to 3 or the moss biochar-microorganism complex obtained by the preparation method described in any one of claims 4 to 6.

8. The composition according to claim 7, characterized in that The substrate includes one or more of peat soil, bone meal, nutrient soil and bran; Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the peat soil is 100g:5kg.

9. Use of the moss biochar-microorganism complex described in any one of claims 1 to 3, the moss biochar-microorganism complex obtained by the preparation method described in any one of claims 4 to 6, or the composition described in claim 7 or 8 in improving saline-alkali land and / or improving the salt-alkali tolerance of plants.

10. A method for improving saline-alkali land and / or improving the salt-alkali tolerance of plants, characterized in that: Include one or both of the following: (1) mixing the moss biochar-microorganism complex described in any one of claims 1 to 3 or the moss biochar-microorganism complex obtained by the preparation method described in any one of claims 4 to 6 with a substrate to obtain a moss biochar-growth-promoting microorganism complex-substrate; applying the moss biochar-growth-promoting microorganism complex-matrix to saline-alkali soil; Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the substrate is 100 g:5 kg; The application amount of the moss biochar-growth-promoting microorganism complex-substrate is 100 kg / mu; The substrate includes one or more of peat soil, bone meal, nutrient soil and bran; (2) Applying the composition of claim 7 or 8 to saline-alkali soil; the application amount of the composition is 100 kg / mu.

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