46 results about "Fungus resource" patented technology

The invention provides a method for preparing black fungus nutrient solution by utilizing food processing residues. The method comprises the following steps: carrying out liquid culture and solid fermentation based on carrot residue as a main raw material for solid fermentation of black fungus and black fungus as a starting strain so as to obtain a large amount of fermented black fungus mycoplasm; then digesting fermented mycoplasm with a hot water digestion method so as to obtain extract rich in black fungus polysaccharide and amino acid, and preparing the extract into a concentrated liquid,wherein when the content of a soluble solid state material in the concentrated liquid is 6%, the content of polysaccharide can reach 3.3%, and the content of amino acid can reach 1150.596mg / 100mL; and finally, blending the concentrated liquid into a nutrient solution which is good in palatability. By application of the method, the food processing residues can be sufficiently utilized, natural resources can also protected, the problem that the wild black fungus resources are significantly in lack is relieved, and the black fungus nutrient solution can partially substitute for black fungus sporocarp for application.

The invention discloses a method for artificially cultivating breeding funguses of an umbellate pore fungus, which solves the problem that the traditional umbellate pore fungus breeding fungus resource is critically lacked. The method provided by the invention comprises the following steps of: (1) breeding main materials: selecting fresh, black and elastic umbellate pore fungus sclerotiums with individual weights of more than 15 g as stock spawns; culturing pure-breed slant breed of the umbellate pore fungus into a cultivating breed; culturing a shoestring fungus slant breed into the cultivating breed and a strain rod; (2) producing a sclerotium excitant; (3) filling the cultivating breed into a basket to be cultivated; (4) managing cultivation; and (5) harvesting and carrying out a multiple cropping. The amount of the breeding funguses which are harvested by the method for two times is 4 times as much as the amount of the added sclerotium; the time is shortened to 14 months from the previous 28 months; the output of the good-quality grey funguses of the breeding funguses is great, the growing time can be shortened after the breeding funguses are planted in large fields, the growing time can be shortened to 3-4 years from the previous 4-5 years, and the breeding funguses have the high and stable yields; and the method provided by the invention realizes the aims of rapidness, high yield and low consumption, and can repeatedly harvest the breeding funguses of the umbellate pore fungus.

The invention relates to a tissue separation medium for wild domestic fungus strain and a preparation method. A base medium is prepared according to the following formula: in parts by mass, 20-30 parts of glucose, 5-7 parts of peptone, 2-5 parts of potassium dihydrogen phosphate, 1-3 parts of magnesium sulfate, 15-20 parts of agar powder, and 1000 parts of water; the base medium also comprises 0.05%-0.20% of an antifungal medicine according to the mass percent concentration; and in the base medium which is added with the antifungal medicine and is subjected to sterilization and cooling, per one liter of the medium also comprises 500 units-10000 units of two antibiotics. After the base medium is stirred uniformly, the base medium is subpackaged into plastic test tubes with covers under aseptic condition when the base medium is hot, and the subpackaged base medium is cooled for usage. After being sent to a laboratory, a wild strain resource material acquired by employing the medium disclosed by the invention is strong in vitality, less in infectious microbe and easy to purify, the medium helps to provide a reliable effective technological means for obtaining wild domestic fungus resource, and has extremely high application value.

The invention relates to a Pleurotus tuber-regium protoplast preparation method, and belongs to the technical field of bioengineering. According to the invention, cellulase is selected for enzymolysis according to the composition of glycosidic bonds of Pleurotus tuber-regium cell wall polysaccharides, Pleurotus tuber-regium protoplasts are effectively obtained, the number can reach 5 * 10 < 6 > / mL, and the regeneration rate reaches 11%. The method provided by the invention has the advantages of simple process and strong operability, and has potential application prospects in the fields of edible and medicinal fungus resource development, genetic breeding, molecular biology, microbiology and the like.

The invention discloses cyclic dipeptide C5 with anti-avian influenza activity, and the cyclic dipeptide C5 is separated from fermention broth and fruiting body of phellinus igniarius (L ex Fr) Quel (phellinus linteus (Berk et Curt) Teng, Phellinus baumii and Phellinus hartigii (Allesch et Schnabl)Imaz). A lead compound is provided to research and development of anti-avian influenza medicines, and the application has an important meaning to development and utilization of medical fungus resources.

The invention relates to the technical field of medicament, in particular to inonotus obliquus extracellular and intracellular mixing crude polysaccharide with a function of strengthening immunity, which is extracted from a submerged fermentation product of medical fungus inonotus obliquus liquid. The method comprises the following steps: taking slant strain under an asepsis condition, and inoculating the slant strain into a plate filled with fresh comprehensive solid culture medium; pumping and filtering a secondary fermentation product through primary fermentation and secondary fermentationto obtain inonotus obliquus mycelium and fermentation supernatant; and preparing the inonotus obliquus extracellular and intracellular mixing crude polysaccharide through concentration and water extraction and alcohol precipitation. In vivo experiments prove that the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can prolong the surviving time of mice hepatocellularcarcinoma H22 and S180 ascites tumor mice, and can obviously improve the thymus gland index and spleen index of the mice. Furthermore, the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can obviously improve secretion of IL-2, TNF-alpha cell factors in the blood serum of the tumor-bearing mice, and can be used for preparing new immunity intensifying preparation. Theinvention opens up a new way for development and utilization of medical fungus resources.