Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity

A technology of Inonotus obliquus and external mixing, applied in the field of medicine, can solve the problems of the limitation of development and utilization of Inonotus obliquus, the high price of Inonotus obliquus, etc.

Active Publication Date: 2012-10-31
王琦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] The present invention provides a mixed intracellular crude polysaccharide of Inonotus obliquus with the effect of enhancing immunity and its medicinal application, so as to solve the problem that wild Inonotus obliquus is very expensive and the development and utilization of Inonotus obliquus is limited

Method used

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  • Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity
  • Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity
  • Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Embodiment 1 Preparation of tablet of the present invention

[0094] 1. Under sterile conditions, take the slant strains and insert them into a plate of fresh comprehensive solid medium, culture at 26°C, and cultivate for 240 hours; the comprehensive solid medium formula (g / 100ml): potato 16, glucose 1.2, agar 1.5, MgSO 4 0.12, KH 2 PO 4 0.28, VB 1 Add 0.8mg of water to dissolve to the required volume, and the pH value is natural;

[0095] 2. Primary fermentation: under aseptic conditions, use a 6mm hole puncher to punch holes at the edge of the colony, use an inoculation shovel to pick up 6 pieces of bacterial cakes and insert them into a 250ml shaker flask containing 100ml of liquid medium, and place at 26°C , the rotating speed is in the shaker of 120rpm / min, after 210 hours of constant temperature cultivation, carry out secondary fermentation culture; the liquid medium formula (g / 100ml): potato 16, glucose 1.2, MgSO 4 0.12, KH 2 PO 4 0.28, VB 1 Add 0.8mg of ...

Embodiment 2

[0099] Embodiment 2: the preparation of granule of the present invention

[0100] 1. Under aseptic conditions, take the slant strains and insert them into a plate of fresh comprehensive solid medium, culture at 28°C, and cultivate for 300 hours; the comprehensive solid medium formula (g / 100ml): potato 21, glucose 2.4, agar 2.0, MgSO 4 0.16, KH 2 PO 4 0.31, VB 1 Add 1.0mg of water to dissolve to the required volume, and the pH value is natural.

[0101] 2. Primary fermentation: under aseptic conditions, use a 6mm hole puncher to punch holes at the edge of the colony, use an inoculation shovel to pick up 8 pieces of bacterial cakes and insert them into a 250ml shaker flask containing 120ml of liquid medium, and place at 28°C , the rotating speed is in the shaker of 150rpm / min, after 260 hours of constant temperature cultivation, the secondary fermentation culture is carried out; the liquid medium formula (g / 100ml): potato 21, glucose 2.4, MgSO 4 0.16, KH 2 PO 4 0.31, VB ...

Embodiment 3

[0105] The preparation of embodiment 3 capsules of the present invention

[0106] 1. Under sterile conditions, take the slant strains and insert them into a plate of fresh comprehensive solid medium, culture at 29°C, and cultivate for 360 hours; the comprehensive solid medium formula (g / 100ml): potato 26, glucose 3.2, agar 2.5, MgSO 4 0.2, KH 2 PO 4 0.35, VB 1 Add 1.2mg of water to dissolve to the required volume, and the pH value is natural;

[0107] 2. Primary fermentation: Under sterile conditions, use a 6mm hole puncher to punch holes at the edge of the colony, use an inoculation shovel to pick up 10 pieces of bacterial cakes and insert them into a 250ml shaker flask containing 150ml of liquid medium, and place at 29°C , rotating speed is in the shaker of 185rpm / min, carry out secondary fermentation culture after 300 hours of constant temperature cultivation; The formula of this liquid culture medium (g / 100ml): Potato 26, glucose 3.2, MgSO 4 0.2, KH 2 PO 4 0.35, VB ...

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Abstract

The invention relates to the technical field of medicament, in particular to inonotus obliquus extracellular and intracellular mixing crude polysaccharide with a function of strengthening immunity, which is extracted from a submerged fermentation product of medical fungus inonotus obliquus liquid. The method comprises the following steps: taking slant strain under an asepsis condition, and inoculating the slant strain into a plate filled with fresh comprehensive solid culture medium; pumping and filtering a secondary fermentation product through primary fermentation and secondary fermentationto obtain inonotus obliquus mycelium and fermentation supernatant; and preparing the inonotus obliquus extracellular and intracellular mixing crude polysaccharide through concentration and water extraction and alcohol precipitation. In vivo experiments prove that the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can prolong the surviving time of mice hepatocellularcarcinoma H22 and S180 ascites tumor mice, and can obviously improve the thymus gland index and spleen index of the mice. Furthermore, the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can obviously improve secretion of IL-2, TNF-alpha cell factors in the blood serum of the tumor-bearing mice, and can be used for preparing new immunity intensifying preparation. Theinvention opens up a new way for development and utilization of medical fungus resources.

Description

technical field [0001] The invention relates to a mixed intracellular and extracellular crude polysaccharide of Inonotus obliquus with the effect of enhancing immunity. The crude polysaccharide has therapeutic effects in the prevention and treatment of tumors, the strengthening of the weak and sick, and low immune function, and belongs to the technical field of medicine. Background technique [0002] Inonotus obliquus is also known as Inonotus obliquus and Inonotus obliquus in China. Also known as Phaeoporus obliquus, Fuscoporia obliqua, Chaga, Black birch tinder (tuchwood), birch mushroom, Kabanoanoanatake, etc. It belongs to Basidiomycotina, Hymenomycetes, Aphyllophorales, Hymenoehaetaeeae. [0003] Morphology of Inonotus obliquus: Its fruiting bodies are tumor-like (sterile lumps), 25 to 40 cm in diameter, dark in color, deeply cracked on the surface, very hard, and brittle when dry. The spores are broadly elliptic to ovate, smooth, with bristles (setae). The mycelium ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00A61K31/715A61P37/04C12R1/645
Inventor 王琦王金玲潘景芝李玉
Owner 王琦
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