Compound acaromycin A and preparation method thereof and application of compound in preparation of antitumor drug

A technology of anti-tumor drugs and compounds, applied in the field of biology and medicine, can solve the problems of secondary metabolite research without Acaromyces fungi, and achieve good inhibitory effect

Inactive Publication Date: 2016-05-25
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Literature review shows that so far there is no research on the secondary metabolites of Acaromyces fungi, so it is necessary to conduct in-depth research on the secondary metabolites

Method used

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  • Compound acaromycin A and preparation method thereof and application of compound in preparation of antitumor drug
  • Compound acaromycin A and preparation method thereof and application of compound in preparation of antitumor drug
  • Compound acaromycin A and preparation method thereof and application of compound in preparation of antitumor drug

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Experimental program
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Effect test

Embodiment 1

[0032] The deep-sea fungus Acaromycesingoldii FS121 of activation is inserted into the potato dextrose liquid culture medium containing mass fraction 1.5% sea salt (every liter contains 15g sea salt, 200g potato, 20g of glucose, the remainder is water, and its preparation method is: the potato is washed and peeled, Cut to about 1cm in size 3 Weigh 200g of small pieces, put them in 1L of water and boil for 20-30 minutes, filter with double gauze, take the filtrate, add 15g of sea salt and 20g of glucose to the filtrate, add water to make up 1L, and sterilize for later use. ), 28°C, 120rpm, cultured for 5 days to obtain seed solution, and the seed solution was inserted into a new potato glucose liquid medium containing 1.5% sea salt in mass fraction with an inoculum size of 10% by volume, at 28°C, under 120rpm Shaking culture was carried out for 7 days to obtain a fermentation culture.

[0033] The fermentation broth and mycelia of the fermentation culture are separated, the fe...

Embodiment 2

[0041] The growth inhibitory activity of compound acaromycin A on tumor cells was tested by SRB method (SkehanP. et al. 1990).

[0042] The tumor cell lines are glioma cell SF-268, breast cancer cell MCF-7, non-small cell lung cancer cell NCI-H460 and liver cancer cell HePG-2.

[0043] 1. Experimental method: the compound acaromycinA prepared by the present invention is dissolved with dimethyl sulfoxide (DMSO) to obtain a mother solution with a concentration of 10mmol / L, and then diluted to the required concentration with RPMI-1640 medium, and the positive control drug is cisplatin . Take the NCI-H460, SF-268, MCF-7 and HePG-2 cells in the logarithmic growth phase, digest them with trypsin, and count them with trypan blue staining. After the trypan blue exclusion test shows that the cell viability is greater than 95%, use fresh RPMI -1640 medium to adjust the cell concentration to 3×10 4 cells / mL, the cells were seeded in a 96-well plate, 180 μL of cell suspension was added ...

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Abstract

The invention discloses a compound acaromycin A and a preparation method thereof and application of the compound in preparation of an antitumor drug. The compound acaromycin A is separated from an ethyl acetate extract of a fermentation product of a deepsea fungus Acaromyces ingoldii FS121 and has the significant tumor cell growth inhibition activity. Accordingly, a candidate drug is supplied to research and development of the novel antitumor drug, and a scientific basis is supplied to development and utilization of sea fungus resources.

Description

Technical field: [0001] The invention belongs to the field of biology and medicine, and specifically relates to compound acaromycin A, its preparation method and its application in the preparation of antitumor drugs. Background technique: [0002] The ocean has a special environment, especially the deep-sea marine environment. In order to adapt to these special environments such as high salinity, weak alkalinity, low nutrition, and anoxic environment, marine microorganisms inhabit the marine environment, so the secondary metabolites of marine microorganisms have unique structures. types and diverse biological activities. These new chemical entities can not only reveal new biosynthetic pathways, but also inspire people to study biomimetic synthesis and biological activities of these chemical entities. Therefore, marine microbial secondary metabolites have important scientific research and application value, and are important resources for drug development (SunL, et al. 2012)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/92A61K31/352A61P35/00C12P17/06C12R1/645
CPCC07D311/92C12P17/06
Inventor 章卫民高啸巍陈玉婵李浩华刘洪新孙章华李赛妮谭国慧潘清灵叶伟
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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