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Application of fermented Aspergillus niger xj for inhibiting Ralstonia solannacearum of tobacco

A strain of Aspergillus niger, the technology of Aspergillus niger, applied in the application field of Aspergillus niger in inhibiting tobacco bacterial wilt, can solve problems such as deterioration and mold of flue-cured tobacco, increased risk in the field, and danger of post-production of flue-cured tobacco.

Inactive Publication Date: 2014-06-18
GUIZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the comparative document, although the fermentation product is also used, it does not kill the living Aspergillus niger, so it is applied in the field, which greatly increases the risk in the field
In addition, tobacco farmers can easily bring Aspergillus niger into the storage room after curing flue-cured tobacco, and Aspergillus niger pollution is the main factor leading to the deterioration and moldiness of flue-cured tobacco
Therefore, it is extremely dangerous to apply live bacterial liquid to the post-production of flue-cured tobacco!

Method used

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  • Application of fermented Aspergillus niger xj for inhibiting Ralstonia solannacearum of tobacco
  • Application of fermented Aspergillus niger xj for inhibiting Ralstonia solannacearum of tobacco
  • Application of fermented Aspergillus niger xj for inhibiting Ralstonia solannacearum of tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the isolation of pathogenic bacteria

[0025] The isolated strains were artificially cultivated on NA medium and TTC medium respectively, and the bacterial morphology was observed. Among them, several isolated strains had a single colony on NA medium, which was small round, slightly raised, white soup-like and shiny , which is consistent with the description of the growth morphology of R. solanacearum on NA medium; the center of the colony of some isolates on TTC medium is pink, and the surrounding white is more fluid, which is consistent with the description of the growth morphology of R. solanacearum on TTC medium .

Embodiment 2

[0026] Example 2: Identification of pathogenicity

[0027] The strain was transferred from sterile water to NA slant medium, cultured for 48 hours, added sterile water to suspend, poured into a triangular flask containing NA culture medium, and cultured for 48 hours on a constant temperature shaker at 30°C at 180r / min. Bacterial water diluted to 1x10 8 cfu / mL of bacterial suspension.

[0028] Use the leaf axil acupuncture inoculation method: use a sterile injection needle to pierce the vascular bundle in the stem from the leaf axil of the third fully expanded leaf downwards from the top of the tobacco plant with a sterile injection needle, inject 0.2mL of bacterial suspension, then draw out the injection needle, and use Cover the injection site with sterilized and degreased cotton, and then wrap it with a piece of sterilized absorbent cotton to keep it moist for 48 hours. Set up 5 replicates, with 5 plants in each replicate. Use sterile water as a control, and induce the di...

Embodiment 3

[0029] Embodiment 3: antibacterial action assay:

[0030] Training method:

[0031] Experimental strains: Aspergillus niger (Aspergillus niger xj): isolated from the Institute of Fungal Resources, Guizhou University, and is now preserved in the China Center for Type Culture Collection (Address: Wuhan University, Wuhan, China), and the preservation number is CCTCCNO: M206021.

[0032] Tobacco Ralstonia solanacearum: preserved in the Microbiology Laboratory of the College of Life Sciences, Guizhou University.

[0033] PDA slant medium: cut 200g of potatoes into pieces, boil in water for 30min, filter the juice, add 20g of glucose, 20g of agar, make up 1000mL of water, the pH is natural. Sterilization conditions: 121°C, 30min (the following sterilization conditions are the same).

[0034] LB medium (Luria-Bertan medium): tryptone 1%, NaC 10.5%, yeast extract 1%, pH 7.2. Sterilize.

[0035]Tobacco R. solanacearum cultivation method: under aseptic conditions, inoculate tobacc...

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Abstract

This invention relates to an application of fermented Aspergillus niger xj to prevention and control of Ralstonia solannacearum of tobacco. The antagonistic bacteria is Aspergillus niger xj which is separated by Institute of Fungus Resource, Guizhou University and now preserved in the China Center for Type Culture Collection (address: Wuhan University, Wuhan, China) with a preservation number of CCTCCNO: M206021. The Ralstonia solannacearum of tobacco can be effectively prevented and controlled by fermented Aspergillus niger xj disclosed by the invention.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the use of Aspergillus niger xj in inhibiting tobacco bacterial wilt. Background technique [0002] Tobacco bacterial wilt is an important soil-borne disease of tobacco. It usually infects the roots of tobacco, causing diseases of crop roots and even the whole plant, resulting in significant economic losses. Tobacco bacterial wilt is a systemic disseminated disease caused by Ralstonia solanacearum. Once the tobacco plant is infected, the whole plant often dies. The damage is often devastating, so it often causes significant economic losses to tobacco production. Tobacco R. solanacearum mainly survives the winter in the soil along with the plant residues, and can also survive the winter in the seeds or other hosts in the field. The soil with bacteria, diseased tissues and organic fertilizers containing pathogens are the main sources of primary infection of the disease. , The spread o...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P1/00C12R1/685
Inventor 李祝丛铭万科葛永怡陈雪
Owner GUIZHOU UNIV
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