Method for extracting chromone and naphthol compounds from phorbia fungi

A compound and fungal technology, applied in the separation/purification of carbonyl compounds, organic chemical methods, organic chemistry, etc., to achieve high purity, enrich raw material sources, and promote extensive development

Active Publication Date: 2022-04-19
HENAN UNIVERSITY
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  • Application Information

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Problems solved by technology

[0004] However, there is no fermentation isolation of excellent ketones and naphthols such as (2R) 5-hydroxy-8-methoxy-2-methyl-4H-1-benzopyran- 4-keto, 5,8-hydroxy-2methyl-4H-1-benzopyran-4-one, (4S) 4,5-dihydroxy-3,4-dihydro-1(2H)- Reports about ketones, etc.

Method used

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  • Method for extracting chromone and naphthol compounds from phorbia fungi
  • Method for extracting chromone and naphthol compounds from phorbia fungi
  • Method for extracting chromone and naphthol compounds from phorbia fungi

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Embodiment 1

[0040] A method for extracting chromones and naphthols from the fungus of the genus Carcharomyces genus, comprising the following steps:

[0041] 1) Fungal culture: Inoculate about 5ml of the fungal seed solution into an 800 × 1000 Erlenmeyer flask filled with rice culture medium, and cultivate it at room temperature for 30 days; the rice culture medium is composed of 80g of rice mixed with 70ml of 0.3% saline;

[0042] 2) Preparation of crude extract: soak and extract the product obtained in step 1) in methanol at room temperature for 3 times (the amount of methanol added in each extraction is 25L), 3 days each time, combine the extracts, and concentrate under reduced pressure To reclaim the solvent, obtain about 246.6g of crude extract; the volume concentration of methanol used is 97%;

[0043] 3) Preparation of the extract from the ethyl acetate part: After the crude extract was dissolved in water, it was extracted 3 times with ethyl acetate (the amount of ethyl acetate add...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for extracting chromone and naphthol compounds from phorbia fungi, which comprises the steps of strain activation and culture, crude extract obtaining, silica gel column chromatography and gel column chromatography. The preparation method comprises the following steps: by taking a compound as a raw material, performing magnetic resonance detection on the compound, and comparing and identifying the compound as (2R) 5-hydroxy-8-methoxy-2-methyl-4H-1-benzopyran-4-ketone, 5, 8-hydroxy-2-methyl-4H-1-benzopyran-4-ketone, (4S) 4, 5-dihydroxy-3, 4-dihydro-1 (2H)-ketone and the like through literature. The method is beneficial to conveniently and quickly obtaining a large number of the compounds, provides a new way for obtaining the compounds, and is beneficial to the development and utilization of fungal resources; the method has the advantages of simplicity, convenience, rapidness, environmental protection, high yield, high purity, solvent recycling and the like, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of fungal fermentation and extraction, in particular to a method for extracting chromones and naphthols (such as (2R) 5-hydroxy-8-methoxy-2-methyl- 4H-1-benzopyran-4-one, 5,8-hydroxy-2methyl-4H-1-benzopyran-4-one, (4S) 4,5-dihydroxy-3,4 -dihydro-1(2H)-one, etc.) method. Background technique [0002] Carbophyllum ( Daldinia eschscholtzii ) belong to the Ascomycota fungi. The fungus can produce various types of compounds such as naphthols, chromones, alkaloids, flavonoids, terpenes and other compounds, which have pharmacological activities such as anti-tumor, anti-inflammatory and antibacterial, hypoglycemic, and anti-HPV viruses. [0003] The natural compound (2R) involved in the present invention 5-hydroxy-8-methoxy-2-methyl-4H-1-benzopyran-4-one, 5,8-hydroxy-2methyl-4H- 1-Benzopyrancyclo-4-one was extracted and isolated for the first time from the fungus of Ascomycotaceae, which belongs to chromones. M...

Claims

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Application Information

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IPC IPC(8): C07D311/22C07C45/78C07C45/79C07C49/747
CPCC07D311/22C07C45/78C07C45/79C07B2200/07C07C49/747
Inventor 魏金凤陈岩康文艺王贵声尹震花
Owner HENAN UNIVERSITY
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