Tissue separation medium for wild domestic fungus strain and preparation method

A technology of tissue separation and culture medium, applied in the application, fungi, fertilizer mixture and other directions, can solve the problems of rot, mildew, inability to purify, difficult to obtain strain mycelium, etc., to achieve strong vitality, good effect, strong application effect of value

Inactive Publication Date: 2015-02-04
SHENYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the phenomena in the prior art that rot and mildew occur after the separation of wild edible fungus tissues, it is impossible to purify and it is difficult to obtain the mycelia of the strains, the technical problem to be solved by the present invention is to provide a kind of wild edible fungi strains tissue separation Culture medium and preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step 1) Prepare the basic medium according to the following formula: according to the mass calculation, take 20 grams of glucose, 5 grams of peptone, 2 grams of potassium dihydrogen phosphate, 1 grams of magnesium sulfate, 18 grams of agar powder and 1000 grams of water; mix well and set aside ;

[0023] Step 2) In the basal medium, add carbendazim according to the weight percentage concentration so that the final culture medium also contains 0.05% antifungal drug; make the weight percentage concentration of carbendazim in the medium be 0.05%, add The basal medium of antifungal drugs is sterilized at a temperature of 121°C for 60 minutes;

[0024] Step 3) Wait for the basal medium to cool down to 40°C and quickly add antibiotics, then add penicillin and streptomycin to the medium, and add an equivalent amount of 500 units (U, international unit) of penicillin and streptomycin to each liter of medium. streptomycin;

[0025] Step 4) After stirring evenly, divide into pl...

Embodiment 2

[0029] Step 1) Prepare the basic medium according to the following formula: according to the mass calculation, take 30 grams of glucose, 7 grams of peptone, 5 grams of potassium dihydrogen phosphate, 3 grams of magnesium sulfate, 20 grams of agar powder and 1000 grams of water; mix well and set aside ;

[0030] Step 2) Add carbendazim to the basal medium so that the medium also contains 0.20% antifungal drugs according to the weight percentage concentration; the basal medium added with antifungal drugs is sterilized at a temperature of 121° C. for 60 minutes

[0031] Step 3) Wait for the basal medium to cool down to 35°C and quickly add antibiotics, and add an equivalent amount of 5000 units (U, international unit) of penicillin and streptomycin to each liter of medium;

[0032] Step 4) After stirring evenly, divide into plastic test tubes with lids under aseptic conditions while hot, and fill each tube with 1 / 3 of the total volume, buckle the lid, place on an inclined plane, ...

Embodiment 3

[0036] The difference from Example 1 is that step 1) prepare the basal medium according to the following formula: according to the mass calculation, take 25 grams of glucose, 6 grams of peptone, 4 grams of potassium dihydrogen phosphate, 2 grams of magnesium sulfate, and 15 grams of agar powder and 1000 grams of water; mix well and set aside;

[0037] Step 2) Add carbendazim to the basal medium according to the weight percentage concentration so that the medium also contains 0.10% antifungal drugs; the basal medium added with antifungal drugs is sterilized at a temperature of 121°C for 60 minutes

[0038] Step 3) Wait for the basal medium to cool down to 38°C and quickly add antibiotics, and add an equal amount of 10,000 units (U, international unit) of penicillin and streptomycin to each liter of medium;

[0039] Step 4) After stirring evenly, divide into plastic test tubes with lids under aseptic conditions while hot, and fill each tube with 1 / 3 of the total volume, buckle t...

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Abstract

The invention relates to a tissue separation medium for wild domestic fungus strain and a preparation method. A base medium is prepared according to the following formula: in parts by mass, 20-30 parts of glucose, 5-7 parts of peptone, 2-5 parts of potassium dihydrogen phosphate, 1-3 parts of magnesium sulfate, 15-20 parts of agar powder, and 1000 parts of water; the base medium also comprises 0.05%-0.20% of an antifungal medicine according to the mass percent concentration; and in the base medium which is added with the antifungal medicine and is subjected to sterilization and cooling, per one liter of the medium also comprises 500 units-10000 units of two antibiotics. After the base medium is stirred uniformly, the base medium is subpackaged into plastic test tubes with covers under aseptic condition when the base medium is hot, and the subpackaged base medium is cooled for usage. After being sent to a laboratory, a wild strain resource material acquired by employing the medium disclosed by the invention is strong in vitality, less in infectious microbe and easy to purify, the medium helps to provide a reliable effective technological means for obtaining wild domestic fungus resource, and has extremely high application value.

Description

technical field [0001] The invention relates to the field of processing Cordyceps militaris, in particular to a culture medium and a preparation method for isolating edible fungus strains and tissues in the field. Background technique [0002] Edible fungus strains are important strategic resources. Wild edible fungus resources are an important source of new varieties. Tissue separation technology is a common method to obtain wild edible fungus seed sources. Due to the lack of sterile operating conditions, bacteria often occur during the operation. And mold pollution, often play a selective antibacterial or bactericidal effect by acting on one or some metabolic links of bacteria. The mushroom body tissue blocks obtained by the edible fungus tissue separation technology are rotten and mildewed after being brought back to the laboratory, and cannot be purified at all, and it is difficult to obtain the mycelium of the fungus, which makes it impossible to effectively extract and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
CPCC12N1/14
Inventor 郑立博王升厚林利萍刘一周阳金硕
Owner SHENYANG NORMAL UNIV
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