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95 results about "International unit" patented technology

In pharmacology, the international unit is a unit of measurement for the amount of a substance; the mass or volume that constitutes one international unit varies based on which substance is being measured, and the variance is based on the biological activity or effect, for the purpose of easier comparison across substances. International units are used to quantify vitamins, hormones, some medications, vaccines, blood products, and similar biologically active substances.

Breeding method of loach

The invention discloses a breeding method of the loach, which can be used for improving the spawning expediting rate and the fertilization rate of the loach and the livability of the loach seedlings. The breeding method of the load comprises the steps of: a. respectively breeding the male loach and the female loach a half month before spawning expediting; b. adding a biological odinagogue into forage one week before spawning expediting, wherein the biological odinagogue specifically comprises 15-20g of Chinese angelica, ligusticum wallichii, folium artemisiae argyi, andrographis paniculata, rehmanniae vaporata and compound vitamins respectively, and the adding quantity is that 5-10g of the biological odinagogue is added into each kilogram of the forage, and continuously feeding the forage which is added with the biological odinagogue for 7 days; and c. making sets according to the proportion that the ratio of the female loach to the male loach is 1:1.5, and injecting 100-150 international units of HCG (human chorionic gonadotropin) into each loach. The breeding method has the beneficial effects that the female loach and the male loach are synchronously estrous after the breeding method is used, so that the spawning expediting rate and the fertilization rate of the loach are greatly improved. After a preferable mode of the breeding method is adopted, the cost can be reduced when the seedlings are bred, and the bait with an enough quantity can be timely provided, so that the livability of the loach seedling is guaranteed, and the large-scale production is realized.
Owner:SICHUAN YINGJIE SCI & TECH DEV

SFM (serum-free medium) for culturing MSCs (mesenchymal stem cells)

The invention relates to an SFM (serum-free medium) for culturing MSCs (mesenchymal stem cells). Based on volume, the SFM comprises the following components: 10.2 grams per liter of alpha-MEM (alpha-minimum essential medium), 2.4 grams per liter of sodium bicarbonate, 1 to 5 millimoles of L-glutamine, 50 to 300 milligrams per liter of poloxamer 188, 2 to 8 grams per liter of recombinant human albumin, 10 to 20 milligrams per liter of recombinant human transferrin, 2 to 10 milligrams per liter of recombinant human insulin, 1 to 5 millimoles per liter of Hepes, 50 nanomoles of beta-mercaptoethanol, 0.1 to 1 milligram per liter of lipid, 1 to 5 milligrams per liter of trace element, 0.1 to 5 milligrams per liter of glutathione, 0.5 to 5 milligrams per liter of para-aminobenzoic acid, 1 to 50 nanograms per milliliter of hydrocortisone, 20 to 50 milligrams per liter of vitamin PP, 5 to 50 milligrams per liter of vitamin C, 2 to 10mu M of compound shown in a formula I, 5 to 20mu M of compound shown in a formula II, 10 to 20 nanograms per milliliter of progestin, 1 to 10 milligrams per liter of putrescine, 1 to 10 international units per liter of heparin, 1 to 10 nanograms per milliliter of EGF (epidermal growth factor), 1 to 10 nanograms per milliliter of b-FGF (b-fibroblast growth factor), 1 to 10 nanograms per milliliter of HGF (hepatocyte growth factor) and 1 to 10 nanograms per milliliter of VEGF (vascular endothelial growth factor). The SFM for culturing the MSCs is a BPS-SFM which has determinate chemical components and is free of animal-derived substances.
Owner:BEIJING DONGFANG HUAHUI BIOMEDICAL TECH

Agricultural chemical composition containing fipronil and bacillus thuringiensis

The invention belongs to the pesticide field and particularly relates to a synergic pesticidal composition with active ingredients to be the combinations of fipronil and a series of subspecies of Bacillus thuringiensis (Bt for short), wherein the Bacillus thuringiensis subspecies include six subspecies: Bacillus thuringiensis var.kurstaki, Bacillus thuringiensis var.aizawa, Bacillus thuringiensis var.tianmensis, B.thuringiensis var.israelensis, Bacillus thuringiensis subsp.aizawai and Bacillus thuringiensis subsp. kenyae. The main agent thereof includes the following active ingredients by the weight percentages as follows: fipronil 0.1%-80%, Bacillus thuringiensis 100-100000 IU (international unit) / mg, or 10-1000 billion spore / g, or 0.01-20% toxin protein, and the rest is an accessory ingredient. The pesticidal composition can be efficiently used for controlling agricultural pests such as rice leaf rollers, chilo suppressalis walkers, diamondback moths, prodenia lituras, corn borers, and so on, and has particular characteristics of high efficacy, long lasting period and safe usage, so that the pesticidal composition belongs to environment-friendly pesticides and can prolong the generation of resistance, reduce production cost and increase economic benefit.
Owner:JIANGMEN PLANT PROTECTION

Livestock semen freezing diluent as well as preparation method and application thereof

The invention relates to a livestock semen freezing diluent as well as a preparation method and application of the livestock semen freezing diluent. The preparation method of the livestock semen freezing diluent comprises the following steps of: adding 2.71 grams of trihydroxymethyl aminomethane, 1,4 grams of citric acids, 1.0 gram of monosaccharides, 10 myriad IU (International Unit) of penicillium, 10 myriad IU of streptomycin and 0.29-6.97 grams of inositol compounds to a right amount of ultrapure water; optionally adding 5-20 milliliters of penetrability protectants; uniformly stirring; regulating a pH value to 6.8-7.2; adding 5-20 milliliters of fresh yolk subjected to inactivation treatment; uniformly mixing, and then fixing the volume to 100 milliliters; centrifugalizing at low temperature for 1 hour; and filtering supernate to prepare the livestock semen freezing diluent. Because the inositol compounds can effectively inhibit the generation of ice crystals, the livestock semen freezing diluent disclosed by the invention reduces the mechanical injury caused by the ice crystals on semens, achieves the survival rate of the defrozen semens at about 75%, the activity more than 50%, the acrosome integrality at about 60% and the plasmalemma integrality at about 50% and achieves the non-return rate more than 70% after artificial insemination; and the livestock semen freezing diluent has the advantages of no immune response generation, purity guarantee, low price, and the like by using the inositol compounds as the ice crystal inhibitors of the semen freezing diluent.
Owner:YUNNAN ANIMAL SCI & VETERINARY INST +1

Method for preparing freeze-dried human blood coagulation factor VIII

The invention discloses a method for preparing a freeze-dried human blood coagulation factor VIII. The method comprises the following process of: dissolving by taking water for injection, comprising 3-10 IU (International Unit)/ml of heparin, as a heparin sodium solution and cryoprecipitating; performing PEG (Polyethylene Glycol) precipitation and taking supernatant; performing centrifugal filtration; performing S/D (Solvent/Detergent) inactivation at the temperature of 24-26 DEG C; performing DEAE (Diethylaminoethyl) Sepharose Fast Flow chromatographic column balance, adsorption, washing andelution; performing molecular membrane ultrafiltration; preparing, removing bacteria, sub-packaging, freeze-drying and capping; and dry-heating at the temperature of 99.5-100.5 DEG C and inactivating. In the invention, the process method of combining the PEG precipitation and an ion exchange chromatography technology is adopted; the method is easy and convenient to operate; the F VIII active recovery rate is increased; miscellaneous proteins can be removed on a large scale; the product yield reaches over 60 percent; and the specific activity of the product reaches 5 IU/mg and is obviously greater than a value which is not less than 1 IU/mg stipulated in the pharmacopeia. Meanwhile, the PEG residue is 0.08g/L which is obviously less than the value which is less than or equal to 0.5 IU/mg stipulated in the pharmacopeia, so that Al<3+> residues in the final preparation of an Al(OH)3 gel method are avoided; the product has high purity and high safety; and the quality of the final product is obviously improved.
Owner:NANYUE BIOPHARMING

Preparation method and application of bitter gourd dietary fiber

The invention discloses a preparation method of bitter gourd dietary fibers, which comprises the following steps: (1) washing fresh bitter gourd, cutting the bitter gourd into segments, adding water of which the amount is 4-10 times of the bitter gourd, and pulping; (2) processing the slurry obtained in the step (1) with a colloid mill, and homogenizing at high pressure; (3) heating the slurry to80-100 DEG C and keeping for more than 30 minutes, and reducing the temperature to room temperature; (4) performing ultrasonic treatment on the slurry obtained in the step (3), adjusting the pH valueto 6.5-7.5, and performing enzymolysis by adding protease with specific activity of 1,000-20,000 international units into each kilogram of bitter gourd; (5) after the enzymolysis, adding amylase withspecific activity of 1,000-20,000 international units into each kilogram of bitter gourd; after the enzymolysis is finished, performing enzyme deactivation; and reducing temperature to room temperature; (6) filtering the enzymolysis liquid obtained in the step (5) to obtain a filtrate and filter residue; adding ethanol with the concentration of no less than 95% into the filtrate until the concentration is 60-85%; standing overnight; and centrifuging and filtering to obtain a precipitate; and (7) combining the filter residue and precipitate obtained in the step (6) to obtain the bitter gourd dietary fiber. Through the invention, the purity of the bitter gourd dietary fibers is over 95%, and the bitter gourd dietary fibers have a good effect in regulating blood sugar.
Owner:三沙南海美源岛生物科技有限公司

Method for preparing freeze-dried human blood coagulation factor VIII

The invention discloses a method for preparing a freeze-dried human blood coagulation factor VIII. The method comprises the following process of: dissolving by taking water for injection, comprising 3-10 IU (International Unit) / ml of heparin, as a heparin sodium solution and cryoprecipitating; performing PEG (Polyethylene Glycol) precipitation and taking supernatant; performing centrifugal filtration; performing S / D (Solvent / Detergent) inactivation at the temperature of 24-26 DEG C; performing DEAE (Diethylaminoethyl) Sepharose Fast Flow chromatographic column balance, adsorption, washing andelution; performing molecular membrane ultrafiltration; preparing, removing bacteria, sub-packaging, freeze-drying and capping; and dry-heating at the temperature of 99.5-100.5 DEG C and inactivating. In the invention, the process method of combining the PEG precipitation and an ion exchange chromatography technology is adopted; the method is easy and convenient to operate; the F VIII active recovery rate is increased; miscellaneous proteins can be removed on a large scale; the product yield reaches over 60 percent; and the specific activity of the product reaches 5 IU / mg and is obviously greater than a value which is not less than 1 IU / mg stipulated in the pharmacopeia. Meanwhile, the PEG residue is 0.08g / L which is obviously less than the value which is less than or equal to 0.5 IU / mg stipulated in the pharmacopeia, so that Al<3+> residues in the final preparation of an Al(OH)3 gel method are avoided; the product has high purity and high safety; and the quality of the final product is obviously improved.
Owner:NANYUE BIOPHARMING
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