Weed rice pathogenic bacteria pathogenicity determination method and identification device

Abstract

The invention belongs to the technical field of weed rice pathogenic bacteria determination, and discloses a weed rice pathogenic bacteria pathogenicity determination method and an identification device. The weed rice pathogenic bacteria pathogenicity determination method comprises the following steps of collecting weed rice disease specimens in a rice planting area; separating fungi from stems, leaves and seeds of the preserved strains within 3 days after collection, purifying and preserving the separated fungi, and separating pathogenic fungi infecting the weed rice from air-dried and preserved specimens by using a tissue culture separation method; identifying pathogenic bacteria of the weed rice: culturing the bacterial strain at constant temperature, recording the form, color and growth speed of bacterial colonies, and analyzing spore production conditions and morphological characteristics; and determining pathogenicity of pathogenic bacteria of the weed rice. According to the method, a Ningxia weed rice pathogenic fungus resource library is established, pathogenic fungus resources which have strong pathogenicity to weed rice and do not have pathogenicity to the rice or have little influence on the rice are screened, and a basis is provided for developing fungus herbicides to prevent and control the weed rice.

Description

technical field [0001] The invention belongs to the technical field of determination of weedy rice pathogenic bacteria, and in particular relates to a pathogenicity determination method and identification device of weedy rice pathogenic bacteria. Background technique [0002] At present, weedy rice, also known as wild rice and miscellaneous rice, is a global weed damage. Weedy rice associated with cultivated rice is widely distributed in many countries and regions in the world. Their morphological characteristics are between wild rice and cultivated indica and japonica rice. , most weedy rices are very similar to wild rices, mature seeds are light, dormant and easy to shatter, and the tillering and flowering stages are generally earlier than the associated cultivated rice varieties. Compared with cultivated rice, weedy rice is in the competition The dominance of nutrients, water and light will result in a reduction in rice yield and quality. In rice production, the yield red...

AI Technical Summary

Problems solved by technology

Its existence has poor effect on the control measures of weedy rice, and there are few investigations, identifications and pathogenicity studies on pathogenic bacteria of weedy rice, and the main diseases and pathogenic bacteria types of weedy rice cannot be ascertained, and the resource bank of pathogenic fungi of weedy rice cannot be established , can not provide a basis for the development of fungal herbicides to control weedy rice

[0005] Through the above analysis, the existing problems and defects of the prior art are: the prior art has poor effect on the control measures of weedy rice, less research on the investigation, identification and pathogenicity of weedy rice pathogens, and it is impossible to ascertain the pathogenicity of weedy rice. The main diseases and types of pathogenic bacteria cannot establish a resource bank of weedy rice pathogenic fungi, and cannot provide a basis for the development of fungal herbicides to control weedy rice

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