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Method for identifying sugarcane smut resistance

A technology for resistance identification and smut, which is applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of small workload, long identification period, and large amount of teliospores, etc. Achieve the effect of small workload, short identification period and high sensitivity

Inactive Publication Date: 2012-05-23
GUANGZHOU SUGARCANE IND RES INST +1
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Problems solved by technology

[0007] The purpose of the present invention is to propose a sugarcane smut resistance identification for existing identification of sugarcane smut resistance. method, this method has the characteristics of short duration, small workload, high efficiency and rapidity, and can also identify the physiological race types of sugarcane smut resistance of the tested varieties, optimize the breeding of sugarcane resistance to smut, and improve the prevention and control of sugarcane smut Effect

Method used

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Embodiment Construction

[0037] A method for identifying resistance to sugarcane smut is carried out according to the following steps:

[0038] Step A. Isolation and culture of heterotypic (+, -) haploid basidiomycetes

[0039] A1. In May 2010, the newly extracted sugarcane smut single whip was collected, and a small amount (approximately 8μg) of fresh sugarcane smut fungus teliospores were picked up on a clean bench on a clean bench and added to the YEPS liquid medium (1mL) After fully shaking into a suspended turbid solution, suck 100μL of turbid solution and YEPs liquid medium (2 mL) to fully dilute to obtain a uniform dilution. There are 50-100 spore spores of smut spores in each milliliter of dilution.

[0040] A2. Aspirate 100 μL of the diluted solution and evenly coat the plate on the YEPS solid medium, and incubate at 28-30°C for 2 to 3 days; a white "wool-like" single colony is obtained.

[0041] A3. Pick 1 white "woollike" single colony and fully dilute it with YEPS liquid medium to a dilution of ...

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Abstract

The invention relates to the technical field of plant disease resistance, and discloses a method for identifying sugarcane smut resistance. The method for identifying sugarcane smut resistance comprises the steps of: firstly carrying out separate culture to obtain sugarcane smut alien mating type (+or -) haplospores by using a trace amount of fresh sugarcane smut teliospore; and then carrying outthe steps of culture enlargement of strains, inoculation, investigation of disease incidence, resistance evaluation and the like. The method for identifying sugarcane smut resistance has the outstanding substantial characteristics and is improved remarkably as follows: 1. the inoculation need can be met by separate culture of only an extremely trace amount of sugarcane smut teliospore, and the working amount is low; 2. the alien mating type strains can be stored at an ultra-low temperature and can be used as the inoculation source after subjected to activation and enlarge culture without repeated taking of strains and separate culture for each time; 3. in the method for identifying sugarcane smut resistance, the resistance identifying period is short, and the sensitivity is high; and 4. the method for identifying sugarcane smut resistance can also be used for identifying the type of smut biological strains resisted by the tested varieties, and effectively guiding smut-resisting seed breeding of sugarcane and reasonable collocation of varieties in a sugarcane area.

Description

Technical field [0001] The invention relates to the technical field of plant disease resistance identification, in particular to a method for identifying resistance to sugarcane smut, and also relates to a method for identifying physiological races of smut. Background technique [0002] From the sugarcane smut ( U. scitaminea ) Sugarcane smut is an important sugarcane disease worldwide and one of the most economically harmful sugarcane diseases in my country. The most economical and effective way to prevent and control this disease is disease-resistant breeding. The basis and key of disease-resistant breeding is the method of disease resistance identification, which is to screen out high-resistant varieties of sugarcane smut for breeding. The commonly used sugarcane smut resistance identification method in our country is to use artificially collected smut spores mixed with teliospores to inoculate the sugarcane smut. The ear disease resistance grading standard (1 to 5 classifica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18A01G1/00A01G7/06
Inventor 沈万宽姜子德陈健文习平根邓海华刘睿
Owner GUANGZHOU SUGARCANE IND RES INST
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