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Chitosan oligosaccharide containging pesticide composition and its application

A pesticide composition and the technology of the composition are applied in the field of agriculture and can solve problems such as the lack of chitosan oligosaccharides.

Inactive Publication Date: 2004-08-18
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no report that oligochitosan is used in agriculture
[0008] On the other hand, various chemical pesticides have more or less various adverse effects on the environment and human beings. Therefore, there is an urgent need in this field to develop new biopesticides that can improve plant disease resistance.

Method used

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  • Chitosan oligosaccharide containging pesticide composition and its application
  • Chitosan oligosaccharide containging pesticide composition and its application
  • Chitosan oligosaccharide containging pesticide composition and its application

Examples

Experimental program
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Effect test

preparation example Construction

[0039] The preparation method of chitosan oligosaccharides is known, and a preferred solution is as follows: solid chitin or clam shells, shrimp shells are washed, dried and crushed powder → press 1:10 in a sealed mixer with cooling facilities Add concentrated hydrochloric acid → 30-45°C and stir for 3-12 hours until all the chitin is dissolved → cool the supernatant suspension in a low-temperature container to 10-20°C → gradually add 5N NaOH to pH 3.0 to produce NaCl precipitation, and Soluble oligosaccharide amine in the supernatant → filter to remove salt (salt can be recycled as industrial salt) → oligosaccharide amine solution is dehydrated and dried to become the finished product → powder is dried and stored.

[0040] The composition of the present invention usually contains a total amount of 0.0005 to 99% by weight of active ingredients (i.e., chitooligosaccharides). Concentrated pesticides have a relatively high active ingredient content, such as 80-90%, while diluted pest...

Embodiment 1

[0063] Preparation of rice suspension cells

[0064] Rice suspension cells were cultured in the dark on a 26°C shaker (80 rpm) in N6 liquid medium, and the fresh medium was replaced every 7-10 days.

[0065] The formula of N6 liquid medium is as follows:

[0066] KNO 3 2.83g / L (NH 4 ) 2 SO 4 0.463g / L

[0067] KH 2 PO 4 0.4g / L MgSO 4 ·7H 2 O 0.185g / L

[0068] CaCl 2 ·2H 2 O 0.166g / L MnSO 4 ·H 2 O 3.3mg / L

[0069] ZnSO 4 ·7H 2 O 1.5mg / L H 3 BO 3 1.6mg / L

[0070] KI 0.8mg / L

[0071] FeSO4·7H 2 O 28mg / L Na 2 EDTA 37mg / L

[0072] Niacin (B5) 0.5mg / L Thiamine·HCl(B1) 0.1mg / L

[0073] Pyridoxine HCl(B6) 0.5mg / L Inositol ester 100mg / L

[0074] Sucrose 30g / L 2,4-D 2mg / L

[0075] Proline 2.3g / L Tryptophan 50mg / L

[0076] pH5.8

Embodiment 2

[0078] Preparation of Chitooligosaccharides and Mother Liquor

[0079] The preparation process of chitooligosaccharides is as follows:

[0080] Solid chitin or clam shells, shrimp shells washed, dried and crushed powder → Add concentrated hydrochloric acid at a ratio of 1:10 in a sealed mixer with cooling facilities → Stir at 45°C for 3-12 hours until all the chitin is Dissolve → cool the supernatant suspension in a low-temperature container to 10-20°C → gradually add 5N NaOH to pH 3.0 to produce NaCl precipitation, while the soluble oligosaccharide amine is in the supernatant → filter to remove the salt (salt can be recovered for industrial use Salt) → oligosaccharide amine solution dehydration and drying is the finished product → powder dry storage.

[0081] For example, 0.2g of chitin (Sigma) is degraded overnight with hydrochloric acid, adjusted to pH 3-4 with 5N NaOH, filtered, desalted, dehydrated and dried to prepare a 10mg / ml oligosaccharide water-soluble mother liquor, 0....

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Abstract

The present invention discloses one kind of pesticide composition containing oligosaccharide in effective amount and carrier. The present invention also discloses its preparation process and use in resisting plant diseases. The composition of the present invention can stimulate the defense reaction of rice and other plants to strengthen disease resistance.

Description

Technical field [0001] The invention relates to the field of agriculture, and more specifically to a pesticide composition containing chitosan oligosaccharide and a preparation method thereof. Background technique [0002] Plants and pathogenic bacteria follow the "gene-to-gene" model to recognize and conduct each other through signals (Flor, HH (1971) Current status of the gene-for-gene concept. Annu. Rev. Phytopathol. 9, 275-296) , And mobilize their respective response systems. As a result of their mutual competition, plants are susceptible, resistant or resistant to pathogens. [0003] In terms of signal transduction pathways, the corresponding R and Avr protein interactions conform to the receptor-ligand mechanism (Baker B, Zambryski P, Staskawicz B, Dinesh-Kumar SP (1997) Signalingin plant-microbe interactions. Science, 276 : 726-733; Lamb CJ (1996) Ligand-receptor mechanism in plant-pathogen recognition. Science, 274: 2038-2039; Martin GB (1999) Functional analysis of plan...

Claims

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Application Information

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IPC IPC(8): A01N31/04
Inventor 何祖华宁伟李群
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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