Application of disease resistance-related protein to regulation and control of plant disease resistance

A technology related to protein and disease resistance, applied in applications, plant peptides, plant products, etc., can solve problems such as natural environment restricting the growth of rice yield

Inactive Publication Date: 2016-08-17
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although experienced the green revolution and the extensive application of hybridization technology, factors such as pests and diseases and harsh natural environment still seriously restrict the growth of rice yield

Method used

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  • Application of disease resistance-related protein to regulation and control of plant disease resistance
  • Application of disease resistance-related protein to regulation and control of plant disease resistance
  • Application of disease resistance-related protein to regulation and control of plant disease resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1, OsAPX8 can regulate the disease resistance of rice

[0089] The present invention provides a disease resistance-related protein derived from rice variety "TP309", and its name is OsAPX8. The amino acid sequence of OsAPX8 is shown in sequence 2 in the sequence listing, the genome sequence of the gene encoding OsAPX8 is shown in sequence 3, the coding sequence of OsAPX8 is shown in sequence 1, the cDNA sequence of OsAPX8 gene is shown in sequence 4 in the sequence listing, and the sequence 4 is Positions 31-1467 are identical to sequence 1.

[0090] 1. Construction of transgenic rice

[0091] 1. Construction of recombinant vector

[0092] The DNA fragment between the EcoRI and XbaI recognition sequences of pCAMBIA1300-35S was replaced with the DNA molecule shown in Sequence 4, and other sequences of the vector were kept unchanged to obtain a recombinant vector, which was named pCAMBIA1300-35S-OsAPX8. pCAMBIA1300-35S-OsAPX8 can express the disease resistance ...

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Abstract

The invention discloses application of disease resistance-related protein to regulation and control of plant disease resistance. In the application disclosed by the invention, the disease resistance-related protein is A1), A2) or A3), wherein A1) is protein with an amino acid sequence shown in the sequence 2; A2) is protein which is obtained by performing the replacement and/or missing and/or addition of one or more of amino acid residues on the amino acid sequence in the sequence 2 and is related to the plant disease resistance; A3) is fusion protein obtained by connecting a tag at the N end or/and the C end of the A1) or A2). An experiment proves that the disease resistance-related protein disclosed by the invention and a coding gene thereof can regulate and control the plant disease resistance; the plant disease resistance can be improved through improving the expression of the disease resistance-related protein, and the plant disease resistance can be reduced through reducing the expression of the disease resistance-related protein, therefore, the plant disease resistance can be regulated and controlled by utilizing the disease resistance-related protein and the coding gene thereof.

Description

technical field [0001] The invention relates to the application of disease resistance-related proteins in regulating plant disease resistance in the field of biotechnology. Background technique [0002] Rice is not only the most important food crop in the world, which is eaten by more than half of the world's population, it is also a model plant for the study of monocots. However, under the situation of decreasing arable land area and expanding population, rice yield has become one of the important factors affecting my country's food security. Although experienced the green revolution and the wide application of hybridization technology, factors such as pests and diseases and harsh natural environment still seriously restrict the growth of rice yield. Bacterial blight is a very serious disease encountered in rice production. After rice is infected with bacterial blight, the yield is generally reduced by 20-30%, or even no harvest. Identifying and isolating rice bacterial b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N5/10C12N15/82A01H5/00
CPCC07K14/415C12N15/8205C12N15/8282
Inventor 江光怀翟文学朱立煌
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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