38results about How to "Reduced disease resistance" patented technology

The invention discloses a malt, pericarpium citri reticulatae and gizzard pepsin growth promotion and diarrhea stopping powder capable of effectively promoting the growth of piglets and preventing diarrhea. The malt, pericarpium citri reticulatae and gizzard pepsin growth promotion and diarrhea stopping powder consists of the following components of 10-15 parts of pericarpium citri reticulatae, 10-15 parts of malts, 10-20 parts of gizzard pepsin, 6-10 parts of rhizoma atractylodis, 5-10 parts of cortex fraxini, 5-10 parts of humifuse euphorbia herb, 6-12 parts of isatis root, 6-10 parts of astragalus membranaceus, 5-10 parts of pickpurse, 2-8 parts of cordate houttuynia, 2-6 parts of allicin, 2-8 parts of galangal and 2-6 parts of liquorice. The malt, pericarpium citri reticulatae and gizzard pepsin growth promotion and diarrhea stopping powder can effectively promote the growth of the piglets, prevent the diarrhea, improve the immunity and improve the survival rate of the piglets, is safe, has no toxic and side effects and no drug resistance and can completely replace antibiotics for health protection of the piglets.

The invention belongs to the field of plant biotechnology, in particular provides a polygalacturonase arrestin gene CaPGIP1 cloned from hot pepper, the gene can specially inhibit the activity of polygalacturonase (PGs) secreted by pathogenic bacteria. The invention provides the function verification techniques based on the coding of the gene, such as protein making, enzyme activity testing, gene silencing, transgenic excessive expression and the like and the application of the techniques. Based on the gene and protein operation technology, the CaPGIP1 gene is proved to effectively participate in the defense reaction of hot pepper under the induction of a stress facto, and can inhibit the activity of polygalacturonase (PGs) secreted by various pathogenic bacteria. The gene silence and transgenic technology are further used for proving that the silence or less expression of the gene in the hot pepper reduces the disease resistance of a host, and the excessive expression of the gene in the transgenic tobacco can enhance the resistance; therefore, the gene codes an important disease-resistance related protein. The invention provides an important technological reserve for transforming the polygalacturonase arrestin (PGIP) gene and cultivating a new disease-resistant variety.

The invention relates to a seed selection method for drought and heat resistant cymbidium hybridum. The method comprises the following steps: (1) carrying out the screening and parent selection on seed sources according to the standard of drought and heat resistance; (2) hybridizing the parents which have the drought and heat resistant characteristic and are selected in the step (1); (3) carrying out the seed asepsis culture; (4) carrying out the filial generation culture; and (5) carrying out the breed screening of the filial generation according to the standard of drought and heat resistance so as to complete the seed selection. The seed selection method for drought and heat resistant cymbidium hybridum can make full use of cymbidium hybridum resources at both home and abroad to culture a new cymbidium hybridum species with the proprietary intellectual property rights. The new cymbidium hybridum has the advantages of draught resistance, high temperature resistance, and adaptation to be implanted in North China.

The invention provides a wide-row double-tree planting method for planting corn. The method comprises the steps of land preparation, seed section, seedling planting, transplanting, field management, harvesting and the like, wherein the seed selection step comprises the following specific steps of: performing sealed seed pregermination on corn coated seeds until seeds sprout, and removing broken and rotten seeds which are not sprouted; the transplanting step comprises the following specific steps of: when seedlings grow to obtain two leaves and one core or three leaves, transplanting the seedlings to a large farmland, wherein a planting row distance is 90-100cm, the hill spacing is 35-40cm, and two seedlings are planted in one hill. According to the method, the row distance is increased, so that the ventilation condition is greatly improved, the solar energy utilization rate is enhanced, improvement of late-stage yield is promoted, field management is relatively convenient, and labor hours are saved; by use of a one-hill double-tree planting mode, the fertilizer utilization rate is improved, and the fertilizer consumption is saved; by virtue of sealed seed pregermination treatment, waste seeds are effectively removed, and the yield is ensured. The method is low in cost, simple to implement and easy to popularize and apply.

The invention provides a cultivating method for increasing content of trace element of pearls. In the water body for cultivating pearls, the content of trace element needed in target pearl cultivationis improved so as to obtain pearls containing one or a plurality of trace elements. The invention overcomes the defects that the trace element is limit in the natural water body environment and the content of trace element of cultivated pearls is low and is even lack. The invention can cultivate pearls which are rich in trace elements or a plurality of trace elements, and the content of the target trace element can be improved by above 20% so as to improve the drug and edible quality of pearls, self-quality and selling price, improve the economic benefit of pearl cultivators and enhance the market competitiveness of the freshwater pearls. The invention is easy and simple to learn, has low cultivating cost and strong applicability, is favorable for popularizing and applying, in particularto various pearl farmers, and increases the financial income of pearl farmers.

The invention discloses a method for cultivating disease-resistant transgenic TaM2K gene wheat and related biological materials. The present invention first protects an important kinase derived from wheat sheath blight resistance, named TaM2K protein, which is a protein composed of the amino acid sequence shown in sequence 1 or sequence 3 in the sequence listing. Nucleic acid molecules encoding TaM2K protein also belong to the protection scope of the present invention. The invention also protects a method for cultivating transgenic plants, which includes the following steps: introducing TaM2K gene into target plants to obtain transgenic plants with higher disease resistance than the target plants. The method for cultivating transgenic plants with improved disease resistance of the present invention has important theoretical and practical significance, and will play an important role in the genetic improvement of plants.

The invention discloses a wheat stripe rust resistance related protein TabZIP74 and coding gene and application thereof. The wheat stripe rust resistance related protein is A1) or A2): A1 is the protein with an amino acid sequence of a sequence 2; A2) substitutes and / or removes and / or adds one or more amino acid residues to the amino acid shown in the sequence 2 in a sequence table and has the protein with the same functions of the protein shown the sequence 2. Experiments prove that the disease resistance on stripe rust of plants can be reduced after the coding genes of the stripe rust resistance related protein are interfered so that a transgenic plant with reduced stripe rust resistance can be obtained to be taken as a model plant for screening plant stripe rust resistance medicines. The stripe rust related protein and the genes thereof have significant meaning on culturing stripe rust resistance plants and are suitable for popularization and use.

The invention relates to a seed selection method for drought and heat resistant cymbidium hybridum. The method comprises the following steps: (1) carrying out the screening and parent selection on seed sources according to the standard of drought and heat resistance; (2) hybridizing the parents which have the drought and heat resistant characteristic and are selected in the step (1); (3) carryingout the seed asepsis culture; (4) carrying out the filial generation culture; and (5) carrying out the breed screening of the filial generation according to the standard of drought and heat resistance so as to complete the seed selection. The seed selection method for drought and heat resistant cymbidium hybridum can make full use of cymbidium hybridum resources at both home and abroad to culturea new cymbidium hybridum species with the proprietary intellectual property rights. The new cymbidium hybridum has the advantages of draught resistance, high temperature resistance, and adaptation tobe implanted in North China.

The invention belongs to the field of plant biotechnology, in particular provides a polygalacturonase arrestin gene CaPGIP1 cloned from hot pepper, the gene can specially inhibit the activity of polygalacturonase (PGs) secreted by pathogenic bacteria. The invention provides the function verification techniques based on the coding of the gene, such as protein making, enzyme activity testing, gene silencing, transgenic excessive expression and the like and the application of the techniques. Based on the gene and protein operation technology, the CaPGIP1 gene is proved to effectively participatein the defense reaction of hot pepper under the induction of a stress facto, and can inhibit the activity of polygalacturonase (PGs) secreted by various pathogenic bacteria. The gene silence and transgenic technology are further used for proving that the silence or less expression of the gene in the hot pepper reduces the disease resistance of a host, and the excessive expression of the gene in the transgenic tobacco can enhance the resistance; therefore, the gene codes an important disease-resistance related protein. The invention provides an important technological reserve for transforming the polygalacturonase arrestin (PGIP) gene and cultivating a new disease-resistant variety.

The present invention discloses a preparation method of an adsorbent for a puffer feed for improving intestinal tract. The preparation method comprises the following steps: chitosan, mineral oil and asulfuric acid solution are mixed evenly; chitin is added; the materials are stirred evenly; vacuum putting still, glutaraldehyde adding for stirring, filtering, washing, drying, placing in a nitrogen-protected tube-type furnace, temperature rising from room temperature, heat preserving and cooling are conducted to obtain the adsorbent for the puffer feed for improving the intestinal tract. The present invention discloses the puffer feed for improving intestinal tract. The puffer feed comprises the following raw materials in parts by weight: 55-83 parts of imported Peruvian fish meal, 12-24 parts of peanut powder, 21-30 parts of water hyacinth dried powder, 14-23 parts of rice chaff, 25-32 parts of tea seed meal, 10-18 parts of sesame seed meal, 4-12 parts of yeast proteins, 8-16 parts oflocust powder, 1-3 parts of spirulina powder, 1-2 parts of the above adsorbent, 0.5-1.2 parts of stone powder, 0.4-1 part of calcium hydrogen phosphate, 0.1-0.2 part of a probiotic preparation and 1-2parts of a puffer compound premix.

The invention provides microecologically agricultural polyvinyl chloride plastic masterbatch and a seedling growing tray prepared from the same. The masterbatch is prepared from components in parts by weight as follows: 10-40 parts of polyvinyl chloride, 15-25 parts of bentonite, 20-30 parts of kaolin, 10-60 parts of calcium carbonate, 0.1-1 part of a catalyst, 0.1-5 parts of a compatilizer, 0.5-2 parts of a stabilizer, 0.5-5 parts of a lubricant and 0.5-5 parts of mixed probiotics. The prepared seedling growing tray is well formed, is high in strength, not prone to deformation and easy to recover and can be repeatedly used, and the cost in a seedling raising process can be reduced; the added nanoscale bentonite, kaolin and calcium carbonate have the effect of preserving moisture and heat and are beneficial to seedling germination and reduction of irrigation labor intensity; with addition of the probiotics beneficial to seedling growth, seedlings are endowed with certain disease resistant capability, have many root hairs at roots, good root climbing effect as well as thick and strong stems and are high in emergence rate and survival rate, fertilizer application and pesticide spraying are almost unnecessary in the seedling raising process, labor is reduced greatly, and the viable number of the prepared microecologically agricultural polyvinyl chloride plastic masterbatch and seedling growing tray is high.

The invention discloses rice bacterial blight resistance related proteins OsBBR1 and coding genes and application thereof. The rice bacterial blight resistance related proteins OsBBR1 are following (A1), (A2) or (A3), wherein (A1) the proteins have the amino acid sequence of the 240-438 bits in the third sequence, (A2) the proteins are obtained in the process that the amino acid sequence shownin the 240-438 bits of the third sequence in a sequence table is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, and have the same function, and (A3)the fusion proteins are obtained by connecting labels to the N ends or / and the C ends of the (A1) or the (A2). Experiments show that plants transfecting OsBBR1 genes can significantly improve diseaseresistance of plants, the disease resistance of the plants missing the OsBBR1 protein function after gene editing is lowered, which indicates that the OsBBR1 proteins and the coding genes thereof canbe used for improving the disease resistance of the plants and have great significance for cultivating the disease-resistant plants.

The invention relates to a preparation method of an alocasis macrorrhiza leaf powder fermented feed. The fermented feed consists of wet alocasis macrorrhiza leaf powder, wheat bran, ammonium sulphate,dicalcium phosphate, magnesium sulphate, geotrichum candidum, rhizopus oryzae, candida tropicalis, lactobacillus plantarum and the like. According to the preparation method disclosed by the invention, the alocasis macrorrhiza leaf powder which cannot be directly used as a feed raw material is subjected to microorganism solid fermentation and the alocasis macrorrhiza leaf powder fermented feed isobtained, so that hot taste, cyanide and protease restraining factors in the alocasis macrorrhiza leaf powder can be completely removed; and besides, through the microorganism fermentation effects, the kinds and the quantity of beneficial microorganisms in the alocasis macrorrhiza leaf powder are increased, and the content of genuine protein and the content of various enzymes are increased. Through fermentation, the respects in the bioavailability, the palatability and the growth promotion of the alocasis macrorrhiza leaf powder are obviously improved, the disease resistance of livestock and poultry is improved, and the productivity is improved; when the alocasis macrorrhiza leaf powder fermented feed is fed, the breeding cost can be reduced, the breeding benefits can be increased, and theinvestment of cost on enzyme preparations and protein feeds is reduced; and the fermented feed is a novel resource utilization type biologic feed, and has great economic value and social value.