Wheat stripe rust resistance related protein tabzip74 and its coding gene and application

A technology for wheat stripe rust and related proteins, applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of increasing the length and quantity and shortening the incubation period

Active Publication Date: 2021-03-12
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most critical technical bottleneck in this method is the screening and functional discovery of effective disease resistance genes.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Wheat stripe rust resistance related protein tabzip74 and its coding gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1. The protein TabZIP74 related to resistance to stripe rust in wheat can regulate the resistance of wheat to stripe rust

[0055] This embodiment provides a stripe rust resistance-related protein (named TabZIP74) derived from the wheat stripe rust resistance near-isogenic line Taichung29*6 / Yr5. In the gene line Taichung29*6 / Yr5, the CDS sequence of the TabZIP74 gene is sequence 1 in the sequence listing, and positions 5-913 of the sequence 1 encode the TabZIP74 protein.

[0056] Construction of gene interference vector:

[0057] Using the cDNA of the wheat stripe rust-resistant near-isogenic line Taichung29*6 / Yr5 as a template, PCR amplification was performed using primers VIGS-bZIP-F1 / VIGS-bZIP-R1, and the target fragment bZIP-VIGS was recovered and purified; the target fragment bZIP -VIGS was digested with ApaI restriction endonuclease, and bZIP-VIGS digestion product was recovered. The pCaBS-γ vector was linearized with ApaI restriction endonuclease, recov...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a wheat stripe rust resistance related protein TabZIP74 and coding gene and application thereof. The wheat stripe rust resistance related protein is A1) or A2): A1 is the protein with an amino acid sequence of a sequence 2; A2) substitutes and / or removes and / or adds one or more amino acid residues to the amino acid shown in the sequence 2 in a sequence table and has the protein with the same functions of the protein shown the sequence 2. Experiments prove that the disease resistance on stripe rust of plants can be reduced after the coding genes of the stripe rust resistance related protein are interfered so that a transgenic plant with reduced stripe rust resistance can be obtained to be taken as a model plant for screening plant stripe rust resistance medicines. The stripe rust related protein and the genes thereof have significant meaning on culturing stripe rust resistance plants and are suitable for popularization and use.

Description

technical field [0001] The invention belongs to the field of biotechnology, more specifically, the invention relates to the wheat stripe rust resistance-related protein TabZIP74 and its coding gene and application. Background technique [0002] Wheat is a gramineous cereal, which is the second largest food crop in the world after rice. my country is the world's largest producer and consumer of wheat, with an annual planting area of ​​about 24 million hectares and an annual output of nearly 130 million tons. Wheat stripe rust is a worldwide airborne disease caused by wheat stripe rust (Puccinia striiformis f.sp tritici). 30%, almost no harvest at the worst. Breeding disease-resistant varieties is the most economical, safe and effective way to control wheat stripe rust. Currently, it is an effective method to obtain disease-resistant crop varieties through genetic engineering breeding. The most critical technical bottleneck in this method is the screening and function disc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8282
Inventor 蔺瑞明王凤涛李媛媛冯晶徐世昌
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap