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Wheat stripe rust resistance related protein tabzip74 and its coding gene and application

A technology for wheat stripe rust and related proteins, applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of increasing the length and quantity and shortening the incubation period

Active Publication Date: 2021-03-12
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The most critical technical bottleneck in this method is the screening and functional discovery of effective disease resistance genes.

Method used

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  • Wheat stripe rust resistance related protein tabzip74 and its coding gene and application

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Effect test

Embodiment 1

[0054] Example 1. The protein TabZIP74 related to resistance to stripe rust in wheat can regulate the resistance of wheat to stripe rust

[0055] This embodiment provides a stripe rust resistance-related protein (named TabZIP74) derived from the wheat stripe rust resistance near-isogenic line Taichung29*6 / Yr5. In the gene line Taichung29*6 / Yr5, the CDS sequence of the TabZIP74 gene is sequence 1 in the sequence listing, and positions 5-913 of the sequence 1 encode the TabZIP74 protein.

[0056] Construction of gene interference vector:

[0057] Using the cDNA of the wheat stripe rust-resistant near-isogenic line Taichung29*6 / Yr5 as a template, PCR amplification was performed using primers VIGS-bZIP-F1 / VIGS-bZIP-R1, and the target fragment bZIP-VIGS was recovered and purified; the target fragment bZIP -VIGS was digested with ApaI restriction endonuclease, and bZIP-VIGS digestion product was recovered. The pCaBS-γ vector was linearized with ApaI restriction endonuclease, recov...

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Abstract

The invention discloses a wheat stripe rust resistance related protein TabZIP74 and coding gene and application thereof. The wheat stripe rust resistance related protein is A1) or A2): A1 is the protein with an amino acid sequence of a sequence 2; A2) substitutes and / or removes and / or adds one or more amino acid residues to the amino acid shown in the sequence 2 in a sequence table and has the protein with the same functions of the protein shown the sequence 2. Experiments prove that the disease resistance on stripe rust of plants can be reduced after the coding genes of the stripe rust resistance related protein are interfered so that a transgenic plant with reduced stripe rust resistance can be obtained to be taken as a model plant for screening plant stripe rust resistance medicines. The stripe rust related protein and the genes thereof have significant meaning on culturing stripe rust resistance plants and are suitable for popularization and use.

Description

technical field [0001] The invention belongs to the field of biotechnology, more specifically, the invention relates to the wheat stripe rust resistance-related protein TabZIP74 and its coding gene and application. Background technique [0002] Wheat is a gramineous cereal, which is the second largest food crop in the world after rice. my country is the world's largest producer and consumer of wheat, with an annual planting area of ​​about 24 million hectares and an annual output of nearly 130 million tons. Wheat stripe rust is a worldwide airborne disease caused by wheat stripe rust (Puccinia striiformis f.sp tritici). 30%, almost no harvest at the worst. Breeding disease-resistant varieties is the most economical, safe and effective way to control wheat stripe rust. Currently, it is an effective method to obtain disease-resistant crop varieties through genetic engineering breeding. The most critical technical bottleneck in this method is the screening and function disc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8282
Inventor 蔺瑞明王凤涛李媛媛冯晶徐世昌
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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