Wheat stripe rust resistance related protein tantl1 and its coding gene and application

A technology for wheat stripe rust and related proteins, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2022-04-19
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most critical technical bottleneck in this method is the screening and functional discovery of effective disease resistance genes.

Method used

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  • Wheat stripe rust resistance related protein tantl1 and its coding gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. The protein TaNTL1 related to resistance to stripe rust in wheat can regulate the resistance of wheat to stripe rust

[0061] This example provides a stripe rust resistance related protein (named TaNTL1) derived from the wheat stripe rust resistance near isogenic line Taichung29*6 / Yr5. In the gene line Taichung29*6 / Yr5, the CDS sequence of the TaNTL1 gene is sequence 1 in the sequence listing, and positions 89-2020 of sequence 1 encode TaNTL1 protein.

[0062] Construction of gene interference vector:

[0063] Using the cDNA of the wheat stripe rust-resistant near-isogenic line Taichung29*6 / Yr5 as a template, PCR amplification was performed using primers VIGS-NTL1F1 / VIGS-NTL1R1, and the target fragment NTL1VIGS was recovered and purified; the target fragment NTL1VIGS was restricted by ApaI Enzyme digestion is carried out, and the NTL1VIGS digestion product is recovered. The pCaBS-γ vector was linearized with ApaI restriction endonuclease, recovered and puri...

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Abstract

The invention discloses a wheat stripe rust resistance-related protein TaNTL1 and its coding gene and application. The wheat stripe rust resistance-related protein disclosed by the present invention is A1) or A2): A1) the amino acid sequence is the protein of sequence 2; A2) the amino acid sequence shown in sequence 2 in the sequence listing is substituted by one or several amino acid residues And / or missing and / or added and have the same function as the protein shown in Sequence 2. Experiments have proved that the resistance of plants to stripe rust can be reduced by interfering with the coding gene of the anti-stripe rust-related protein of the present invention, and transgenic plants with reduced resistance to stripe rust can be obtained as a model for screening drugs for preventing plant stripe rust plant. The anti-stripe rust-related protein and the gene thereof of the invention have important significance for cultivating stripe-rust-resistant plants and are suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to the wheat stripe rust resistance-related protein TaNTL1 and its coding gene and application. Background technique [0002] Wheat is a gramineous cereal, which is the second largest food crop in the world after rice. my country is the world's largest producer and consumer of wheat, with an annual planting area of ​​about 24 million hectares and an annual output of nearly 130 million tons. Wheat stripe rust is a worldwide airborne disease caused by wheat stripe rust (Puccinia striiformis f.sp tritici). 30%, almost no harvest at the worst. Breeding disease-resistant varieties is the most economical, safe and effective way to control wheat stripe rust. Currently, it is an effective method to obtain disease-resistant crop varieties through genetic engineering breeding. The most critical technical bottleneck in this method is the screening and function di...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84A01H5/00A01H6/46
CPCC07K14/415C12N15/8282
Inventor王凤涛蔺瑞明张慧丽冯晶徐世昌
OwnerINST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI