Stripe rust-resistant related protein TaNTL1 in wheat and encoding gene and application thereof

A related protein and coding gene technology is applied to the wheat stripe rust resistance-related protein TaNTL1 and its coding gene and application fields to achieve the effects of shortening the incubation period and increasing the length and quantity.

Active Publication Date: 2020-07-24
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

The most critical technical bottleneck in this method is the screening and functional discovery of effective disease resistance genes.

Method used

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  • Stripe rust-resistant related protein TaNTL1 in wheat and encoding gene and application thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. The protein TaNTL1 related to resistance to stripe rust in wheat can regulate the resistance of wheat to stripe rust

[0061] This example provides a stripe rust resistance related protein (named TaNTL1) derived from the wheat stripe rust resistance near isogenic line Taichung29*6 / Yr5. In the gene line Taichung29*6 / Yr5, the CDS sequence of the TaNTL1 gene is sequence 1 in the sequence listing, and positions 89-2020 of sequence 1 encode TaNTL1 protein.

[0062] Construction of gene interference vector:

[0063] Using the cDNA of the wheat stripe rust-resistant near-isogenic line Taichung29*6 / Yr5 as a template, PCR amplification was performed using primers VIGS-NTL1F1 / VIGS-NTL1R1, and the target fragment NTL1VIGS was recovered and purified; the target fragment NTL1VIGS was restricted by ApaI Enzyme digestion is carried out, and the NTL1VIGS digestion product is recovered. The pCaBS-γ vector was linearized with ApaI restriction endonuclease, recovered and puri...

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Abstract

The invention discloses a stripe rust-resistant related protein TaNTL1 in wheat and an encoding gene and application thereof. The stripe rust-resistant related protein in the wheat disclosed by the invention is (A1) or (A2): (A1) a protein with an amino acid sequence being a sequence 2, and (A2) a protein with an amino acid sequence obtained by substitution and / or deletion and / or addition of one or more amino acid residues in the amino acid sequence shown in the sequence 2 in a sequence table and with the same function as the protein shown in the sequence 2. An experiment proves that after interfering with the encoding gene of the stripe rust-resistant related protein, the resistance of a plant to stripe rust can be reduced, and a transgenic plant with reduced resistance to the stripe rustcan be obtained to be used as a model plant for screening drugs for preventing the stripe rust of the plant. The stripe rust-resistant related protein and the gene thereof disclosed by the inventionare of great significance in cultivating stripe rust-resistant plants and are suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to the wheat stripe rust resistance-related protein TaNTL1 and its coding gene and application. Background technique [0002] Wheat is a gramineous cereal, which is the second largest food crop in the world after rice. my country is the world's largest producer and consumer of wheat, with an annual planting area of ​​about 24 million hectares and an annual output of nearly 130 million tons. Wheat stripe rust is a worldwide airborne disease caused by wheat stripe rust (Puccinia striiformis f.sp tritici). 30%, almost no harvest at the worst. Breeding disease-resistant varieties is the most economical, safe and effective way to control wheat stripe rust. Currently, it is an effective method to obtain disease-resistant crop varieties through genetic engineering breeding. The most critical technical bottleneck in this method is the screening and function di...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/84A01H5/00A01H6/46
CPCC07K14/415C12N15/8282
Inventor 王凤涛蔺瑞明张慧丽冯晶徐世昌
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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