Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli-resistant associated protein PvMES1, and coding gene and application thereof

Abstract

The invention discloses a Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli-resistant associated protein PvMES1. The associated protein PvMES1 is a protein composed of an amino acid sequence represented by SEQ ID NO2; or is a derived protein obtained by substituting and / or deleting and / or adding one or more amino acid residues to the amino acid sequence represented by SEQ ID NO2 and still having Fusarium oxysporum f. sp. Phaseoli resistance. The invention also discloses a coding gene of the associated protein PvMES1, and an application of the associated protein PvMES1 in the Fusarium oxysporum f. sp. Phaseoli resistance. The PvMES1 protein playing a great role in the resistance reaction of the Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli is disclosed in the invention forthe first time. A plant obtained by overexpressing the coding gene has a significantly enhanced resistance to a Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli pathogen, the expression levelof the gene inoculated with the pathogen is 13.6 times that of the uninoculated gene, the expression level of the gene of the gene silent plant inoculated with the pathogen is low to 0.48 time that of the uninoculated gene, and the disease resistance of the plant is significantly reduced. An effective method is provided for the breeding of Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli-resistant molecules.

Description

technical field [0001] The present invention relates to common bean Fusarium wilt disease resistance related protein PvMES1, coding gene and application thereof. Background technique [0002] Crop disease is one of the important reasons leading to crop yield reduction, and reducing the loss of disease is an important way to increase the yield of food and feed crops. Fusarium oxysporum f.sp phaseoli is a common soil-borne disease pathogen of common bean, which is very serious in the vast bean planting areas of China. Fusarium wilt of bean is a kind of vascular disease of common bean. It is a typical soil-borne fungal disease caused by Fusarium oxysporum transformation of bean. It brings great harm to bean production in a suitable year and area of ​​onset. . The pathogen has been found in many bean planting areas all over the world, and mainly harms the roots, stems and leaves of the bean. The diseased leaves quickly wither and turn yellow, fall off, and the plants eventuall...

AI Technical Summary

Problems solved by technology

Current research on MES genes is mainly focused on model plants such as tobacco (Kumar & Klessig, 2003), Arabidopsis (Vlot et al., 2008) and potato (Manosalva et al., 2010), limited by the lack of relevant genomic information However, there are few reports on the MES gene family of leguminous plants such as common bean

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