Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli-resistant associated protein PvMES1, and coding gene and application thereof

A related protein and coding gene technology, which is applied to the common bean Fusarium wilt disease resistance-related protein PvMES1, coding genes and application fields, can solve the problems of lack of genomics information, rarely reported MES gene family and other problems

Inactive Publication Date: 2018-11-16
LIAONING ACAD OF AGRI SCI
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current research on MES genes is mainly focused on model plants such as tobacco (Kumar & Klessig, 2003), Arabidopsis (Vlot et al., 2008) and potato (Manosalva e

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli-resistant associated protein PvMES1, and coding gene and application thereof
  • Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli-resistant associated protein PvMES1, and coding gene and application thereof
  • Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli-resistant associated protein PvMES1, and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the cloning of the coding gene PvMES1 of disease resistance-related protein in common bean

[0043] 1. Isolation of the gene PvMES1 encoding the disease resistance-related protein

[0044] 1. Extraction of total RNA and purification of mRNA. Common kidney bean (BRB130, National Crop Germplasm Bank) was used as the test material, and the root tissue was sampled and mixed in equal amounts at 0, 24, 48, 72, 96 and 120 hours after inoculation of pathogenic bacteria, respectively. Total RNA was extracted with TRIZOL, mRNA was isolated and purified with oligo(dT) cellulose and cDNA was synthesized.

[0045] 2. Using synthetic cDNA as a template, design primers to isolate the MES1 gene in common kidney bean by RT-PCR method. The primer sequences are as follows:

[0046] MES-F1: TATAGCGAGAAAGGAATC

[0047] MES-R1: AAGTTTGGCTGATGGGTT

[0048] Amplified fragments with a size of about 900 bp were amplified, of which a total of 834 bp at positions 49-882 ​​were ope...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli-resistant associated protein PvMES1. The associated protein PvMES1 is a protein composed of an amino acid sequence represented by SEQ ID NO2; or is a derived protein obtained by substituting and/or deleting and/or adding one or more amino acid residues to the amino acid sequence represented by SEQ ID NO2 and still having Fusarium oxysporum f. sp. Phaseoli resistance. The invention also discloses a coding gene of the associated protein PvMES1, and an application of the associated protein PvMES1 in the Fusarium oxysporum f. sp. Phaseoli resistance. The PvMES1 protein playing a great role in the resistance reaction of the Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli is disclosed in the invention forthe first time. A plant obtained by overexpressing the coding gene has a significantly enhanced resistance to a Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli pathogen, the expression levelof the gene inoculated with the pathogen is 13.6 times that of the uninoculated gene, the expression level of the gene of the gene silent plant inoculated with the pathogen is low to 0.48 time that of the uninoculated gene, and the disease resistance of the plant is significantly reduced. An effective method is provided for the breeding of Phaseolus vulgaris L. Fusarium oxysporum f. sp. Phaseoli-resistant molecules.

Description

technical field [0001] The present invention relates to common bean Fusarium wilt disease resistance related protein PvMES1, coding gene and application thereof. Background technique [0002] Crop disease is one of the important reasons leading to crop yield reduction, and reducing the loss of disease is an important way to increase the yield of food and feed crops. Fusarium oxysporum f.sp phaseoli is a common soil-borne disease pathogen of common bean, which is very serious in the vast bean planting areas of China. Fusarium wilt of bean is a kind of vascular disease of common bean. It is a typical soil-borne fungal disease caused by Fusarium oxysporum transformation of bean. It brings great harm to bean production in a suitable year and area of ​​onset. . The pathogen has been found in many bean planting areas all over the world, and mainly harms the roots, stems and leaves of the bean. The diseased leaves quickly wither and turn yellow, fall off, and the plants eventuall...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/18C12N15/55C12N15/82A01H5/00A01H6/54
CPCC12N9/18C12N15/8282
Inventor 薛仁风丰明陈剑赵阳王英杰李韬庄艳葛维德
Owner LIAONING ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap